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Can you guarantee that your ELISA can quantify ASC oligomers (ASC specks) in the plasma of patients? Does it recognize other CARD or PYD domain containing proteins?
How do you confirm your kit works? I have cell lines that are
ASC positive and ASC knockout, yet a previous kit I tried from another
company did not work. What positive and negative controls can be used
to confirm this kit really detects ASC?