Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
Apoptosis associated speck like protein containing a CARD; Apoptosis-associated speck-like protein containing a CARD; ASC; ASC_HUMAN; CARD 5; CARD5; Caspase recruitment domain containing protein 5; Caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; MGC10332; PYCARD; PYD and CARD domain containing; PYD and CARD domain containing protein; PYD and CARD domain-containing protein; Target of methylation induced silencing 1; Target of methylation-induced silencing 1; TMS 1; TMS; TMS1
Species
Homo sapiens (Human)
Expression Region
1-195aa
Target Protein Sequence
MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant human PYCARD protein is encoded by a recombinant DNA that was cloned into the expression vector and then transformed into the E.coli that supports the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag and C-terminal Myc tag gene to the gene fragment coding for the 1-195aa of the human PYCARD protein. After purification, the product is the recombinant human PYCARD protein. This recombinant PYCARD protein was subjected to the SDS-PAGE determination. Its purity reaches over 85% evaluated by Bandscan software analysis combined with SAS-PAGE. This recombinat PYCARD protein may have applications in cancer research.
PYCARD protein contains an N-terminal PYRIN-PAAD-DAPIN domain and a C-terminal caspase-recruitment domain. PYCARD has been found to mediate inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, PYCARD expression is found on cytoplasm. PYCARD comes in four different isoforms (1-4). The four kinds of isoforms are related to different biological process. Recent studies identified that aberrant hypermethylation-mediated suppression of PYCARD is extremely frequent in prostate cancer. In addition, the critical role for PYCARD has been found in the development of experimental autoimmune encephalomyelitis.