Code | CSB-E09603h |
Size | 96T,5×96T,10×96T |
Trial Size | ![]() |
Have Questions? | Leave a Message or Start an on-line Chat |
Description |
The human AST ELISA Kit is intended to measure human AST in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human AST concentration. The intensity of the colored product is directly proportional to the concentration of AST present in the sample. This human AST ELISA kit has high specificity and excellent sensitivity. AST (GOT1) is an enzyme involved in the malate-aspartate (MA) shuttle that produces cytosolic glutamate by transferring amino residue from aspartate to α-ketoglutarate. It is important for the metabolism of amino acids, Krebs cycle activity, maintenance of NAD+/NADH ratio in cells, generation of purine/pyrimidine bases, urea and protein synthesis, and gluconeogenesis. An increased AST activity may be a sign of tissue damage, plasma membrane bleb formation, enhanced tissue expression, and macroenzymes. Elevated AST levels are commonly associated with inflammatory liver disease, alcoholic liver disease, cirrhosis, cholestatic syndromes, drug toxicity, acute myocardial infarction, septic shock, and skeletal muscle injury/trauma. |
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Target Name | glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Alternative Names | AATC_HUMAN ELISA Kit; Aspartate aminotransferase 1 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase cytoplasmic ELISA Kit; Aspartate aminotransferase cytosolic ELISA Kit; ASTQTL1 ELISA Kit; cAspAT ELISA Kit; cCAT ELISA Kit; cysteine aminotransferase; cytoplasmic ELISA Kit; cysteine transaminase; cytoplasmic ELISA Kit; cytoplasmic ELISA Kit; ec 2.6.1.1 ELISA Kit; GIG 18 ELISA Kit; GIG18 ELISA Kit; Glutamate oxaloacetate transaminase 1 ELISA Kit; Glutamate oxaloacetate transaminase soluble ELISA Kit; Glutamic oxaloacetic transaminase 1 soluble ELISA Kit; GOT 1 ELISA Kit; GOT1 ELISA Kit; Growth inhibiting protein 18 ELISA Kit; SGOT ELISA Kit; Transaminase A ELISA Kit |
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Abbreviation | GOT1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Uniprot No. | P17174 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species | Homo sapiens (Human) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Types | serum, plasma, tissue homogenates | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Range | 0.312 mU/ml - 20 mU/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sensitivity | 0.078 mU/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Assay Time | 1-5h | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Volume | 50-100ul | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Wavelength | 450 nm | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Area | Signal Transduction |
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Assay Principle | quantitative | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Measurement | Sandwich | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Precision |
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Linearity |
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Recovery |
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Typical Data | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Troubleshooting and FAQs |
ELISA kit FAQs | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Storage | Store at 2-8°C. Please refer to protocol. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lead Time | 3-5 working days |
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I would like to know if the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.
Function (From Uniprot) |
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
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Gene References into Functions |
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Subcellular Location | Cytoplasm. |
Protein Families | Class-I pyridoxal-phosphate-dependent aminotransferase family |
Database Links |
HGNC: 4432 OMIM: 138180 KEGG: hsa:2805 STRING: 9606.ENSP00000359539 UniGene: Hs.500756 |