Human Aspartate aminotransferase,AST ELISA Kit

Instructions
Code CSB-E09603h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Alternative Names AATC_HUMAN ELISA Kit; Aspartate aminotransferase 1 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase cytoplasmic ELISA Kit; Aspartate aminotransferase cytosolic ELISA Kit; ASTQTL1 ELISA Kit; cAspAT ELISA Kit; cCAT ELISA Kit; cysteine aminotransferase; cytoplasmic ELISA Kit; cysteine transaminase; cytoplasmic ELISA Kit; cytoplasmic ELISA Kit; ec 2.6.1.1 ELISA Kit; GIG 18 ELISA Kit; GIG18 ELISA Kit; Glutamate oxaloacetate transaminase 1 ELISA Kit; Glutamate oxaloacetate transaminase soluble ELISA Kit; Glutamic oxaloacetic transaminase 1 soluble ELISA Kit; GOT 1 ELISA Kit; GOT1 ELISA Kit; Growth inhibiting protein 18 ELISA Kit; SGOT ELISA Kit; Transaminase A ELISA Kit
Abbreviation GOT1
Uniprot No. P17174
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 mU/ml - 20 mU/ml
Sensitivity 0.078 mU/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 93  
Range % 88-94  
1:4 Average % 95  
Range % 90-99  
1:8 Average % 103  
Range % 97-110  
Recovery
The recovery of human AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-94  
             
             
Typical Data   
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

I would like to know if the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.

A:
Thanks for your inquiry.The kit is developed for the human matrix to quantitatively detect the content of the corresponding protein.
Some components of the kit contain BSA,there is no HIV / AIDS / HCV / HBV / infective material.
All kits are for scientific research use, there is no infectious component and can be used in accordance with conventional biosecurity rules of operation.

Target Data

Function Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Gene References into Functions
  1. high transaminase elevations do not predict severe complications or reflect remnant ischemic area. PMID: 29553104
  2. Missense variant (p.Gln208Glu, rs374966349) in glutamate oxaloacetate transaminase 1 (GOT1) was found, as a putative causal variant predisposing to Familial Macro-aspartate aminotransferase . PMID: 28716744
  3. HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway and GOT1 expression in human pancreatic ductal adenocarcinoma. PMID: 28544376
  4. ALT is a more suitable index than AST for developing a regression model. PMID: 28628604
  5. High AST1 expression is associated with high mortality in hemodialysis patients. PMID: 26333544
  6. GOT1 plays an important role in energy metabolism and ROS balance in chronic acidosis stress in pancreatic tumor cells. PMID: 28455244
  7. Early abnormal liver enzyme levels of aspartate aminotransferase and alanine aminotransferase may increase the prevalence of human cytomegalovirus antigenemia after hematopoietic stem cell transplantation. PMID: 28415934
  8. Elevated preoperative aspartate aminotransferase-to-platelet ratio index may be independently associated with poor overall survival and disease-free survival in hepatocellular carcinoma patients following curative resection PMID: 28351330
  9. Elevated levels of ALT are Associated with Cardiovascular disease. PMID: 27872510
  10. Elevated aspartate aminotransferase level was associated with hepatocellular carcinoma. PMID: 26881519
  11. The serum hepcidin, ferritin, alanine transaminase, aspartate transaminase, gamma-glutamyltransferase and bilirubin were examined in two independent Chinese cohorts consisted of 3455 individuals. PMID: 26290281
  12. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GOT1 rises due to up-regulation of hepatic expression of GOT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
  13. Human GOT1 was expressed in E coli, purified by affinity chromatography and preliminary crystallographic studies were done. PMID: 26003525
  14. We propose the GOT1 expression status as a simple and reliable prognostic biomarker in pancreatic ductal adenocarcinoma PMID: 25595905
  15. Significantly higher GOT levels were found in the 1-20 year age group of type 1 PMID: 24849491
  16. The aim of this study was to establish the reference intervals (RIs) of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate transaminase (AST), and creatinine (CREA) for apparently healthy elderly (Han ethnicity) in Shuyang, China. PMID: 25272068
  17. APRI and AST/ALT are independent predictors for significant fibrosis in chronic hepatitis C patients. PMID: 22352690
  18. This study highlights the necessity of intensive follow-up for those with elevated AST and ALT levels and comorbid alcohol use disorder for preventing excessive natural deaths. PMID: 22242166
  19. we discovered a rare in-frame deletion in GOT1 gene, which inactivates cAST enzyme in the Old Order Amish. PMID: 21900944
  20. Data indicate that the percentages of CD4(+)CXCR5(+) TFH in IA patients were positively correlated with AST. PMID: 21750724
  21. liver enzymes, including AST, were significantly associated with IR according to direct clamp assessment, which were independent of the traditional risk factors in diabetic patients PMID: 20972737
  22. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
  23. mildly elevated in severe acute respiratory syndrome patients during second week after onset of fever. PMID: 15306699
  24. saliva AST and ALP may have roles in development of periodontal diseases PMID: 16681433
  25. PTLP activity is positively associated with serum ALT1, GOT1 and GOT2 in type 2 diabetes mellitus and metabolic syndrome. PMID: 17877759
  26. Abnormal serum aminotransferase values are uncommon in severely obese children in France. PMID: 17907318
  27. Data revealed higher aspartate aminotransferase for women with a family history of diabetes, when adjusted for age and BMI. PMID: 18242760
  28. Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
  29. Neither ALT nor GGT concentrations were correlated with ALP concentration, but AST concentration was moderately correlated with ALP concentration. PMID: 19012630
  30. Results suggest that alanine and aspartate aminotransferase levels and AST/ALT ratio do not seem to be reliable predictors for non-alcoholic steatohepatitis. PMID: 19370784
  31. the management of patients with pyelonephritis should take into account that moderate and self-limited abnormalities in aminotransferase levels are frequent during the acute phase of the disease PMID: 19393479

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Subcellular Location Cytoplasm
Protein Families Class-I pyridoxal-phosphate-dependent aminotransferase family
Database Links

HGNC: 4432

OMIM: 138180

KEGG: hsa:2805

STRING: 9606.ENSP00000359539

UniGene: Hs.500756

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