Product Details

Target Name

glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)

Alternative Names

AATC_HUMAN ELISA Kit; Aspartate aminotransferase 1 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase cytoplasmic ELISA Kit; Aspartate aminotransferase cytosolic ELISA Kit; ASTQTL1 ELISA Kit; cAspAT ELISA Kit; cCAT ELISA Kit; cysteine aminotransferase; cytoplasmic ELISA Kit; cysteine transaminase; cytoplasmic ELISA Kit; cytoplasmic ELISA Kit; ec 2.6.1.1 ELISA Kit; GIG 18 ELISA Kit; GIG18 ELISA Kit; Glutamate oxaloacetate transaminase 1 ELISA Kit; Glutamate oxaloacetate transaminase soluble ELISA Kit; Glutamic oxaloacetic transaminase 1 soluble ELISA Kit; GOT 1 ELISA Kit; GOT1 ELISA Kit; Growth inhibiting protein 18 ELISA Kit; SGOT ELISA Kit; Transaminase A ELISA Kit

Abbreviation

GOT1

Uniprot No.

P17174

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 mU/ml - 20 mU/ml

Sensitivity

0.078 mU/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(mU/ml)	2.283	2.469	2.072	2.060	2.283	2.346
SD	0.044	0.045	0.038	0.038	0.044	0.047
CV(%)	6.607	6.475	6.022	6.041	6.607	6.953

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-88

1:2

Average %

Range %

88-94

1:4

Average %

Range %

90-99

1:8

Average %

103

Range %

97-110

Recovery

The recovery of human AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	90	85-94
EDTA plasma(n=4)	95	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.





mU/ml	OD1	OD2	Average	Corrected
20	2.368	2.401	2.384	2.251
10	1.439	1.442	1.440	1.307
5	1.015	1.006	1.011	0.878
2.5	0.665	0.642	0.654	0.521
1.25	0.441	0.435	0.438	0.305
0.625	0.347	0.351	0.349	0.216
0.312	0.201	0.204	0.203	0.070
0	0.135	0.132	0.133

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The human AST ELISA Kit is intended to measure human AST in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human AST concentration. The intensity of the colored product is directly proportional to the concentration of AST present in the sample. This human AST ELISA kit has high specificity and excellent sensitivity.

AST (GOT1) is an enzyme involved in the malate-aspartate (MA) shuttle that produces cytosolic glutamate by transferring amino residue from aspartate to α-ketoglutarate. It is important for the metabolism of amino acids, Krebs cycle activity, maintenance of NAD⁺/NADH ratio in cells, generation of purine/pyrimidine bases, urea and protein synthesis, and gluconeogenesis. An increased AST activity may be a sign of tissue damage, plasma membrane bleb formation, enhanced tissue expression, and macroenzymes. Elevated AST levels are commonly associated with inflammatory liver disease, alcoholic liver disease, cirrhosis, cholestatic syndromes, drug toxicity, acute myocardial infarction, septic shock, and skeletal muscle injury/trauma.

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Customer Reviews and Q&A

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■ Q&A

I would like to know if the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.

Thanks for your inquiry.The kit is developed for the human matrix to quantitatively detect the content of the corresponding protein.
Some components of the kit contain BSA,there is no HIV / AIDS / HCV / HBV / infective material.
All kits are for scientific research use, there is no infectious component and can be used in accordance with conventional biosecurity rules of operation.

Target Background

Function
(From Uniprot)

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Gene References into Functions

high transaminase elevations do not predict severe complications or reflect remnant ischemic area. PMID: 29553104
Missense variant (p.Gln208Glu, rs374966349) in glutamate oxaloacetate transaminase 1 (GOT1) was found, as a putative causal variant predisposing to Familial Macro-aspartate aminotransferase . PMID: 28716744
HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway and GOT1 expression in human pancreatic ductal adenocarcinoma. PMID: 28544376
ALT is a more suitable index than AST for developing a regression model. PMID: 28628604
High AST1 expression is associated with high mortality in hemodialysis patients. PMID: 26333544
GOT1 plays an important role in energy metabolism and ROS balance in chronic acidosis stress in pancreatic tumor cells. PMID: 28455244
Early abnormal liver enzyme levels of aspartate aminotransferase and alanine aminotransferase may increase the prevalence of human cytomegalovirus antigenemia after hematopoietic stem cell transplantation. PMID: 28415934
Elevated preoperative aspartate aminotransferase-to-platelet ratio index may be independently associated with poor overall survival and disease-free survival in hepatocellular carcinoma patients following curative resection PMID: 28351330
Elevated levels of ALT are Associated with Cardiovascular disease. PMID: 27872510
Elevated aspartate aminotransferase level was associated with hepatocellular carcinoma. PMID: 26881519
The serum hepcidin, ferritin, alanine transaminase, aspartate transaminase, gamma-glutamyltransferase and bilirubin were examined in two independent Chinese cohorts consisted of 3455 individuals. PMID: 26290281
Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GOT1 rises due to up-regulation of hepatic expression of GOT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
Human GOT1 was expressed in E coli, purified by affinity chromatography and preliminary crystallographic studies were done. PMID: 26003525
We propose the GOT1 expression status as a simple and reliable prognostic biomarker in pancreatic ductal adenocarcinoma PMID: 25595905
Significantly higher GOT levels were found in the 1-20 year age group of type 1 PMID: 24849491
The aim of this study was to establish the reference intervals (RIs) of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate transaminase (AST), and creatinine (CREA) for apparently healthy elderly (Han ethnicity) in Shuyang, China. PMID: 25272068
APRI and AST/ALT are independent predictors for significant fibrosis in chronic hepatitis C patients. PMID: 22352690
This study highlights the necessity of intensive follow-up for those with elevated AST and ALT levels and comorbid alcohol use disorder for preventing excessive natural deaths. PMID: 22242166
we discovered a rare in-frame deletion in GOT1 gene, which inactivates cAST enzyme in the Old Order Amish. PMID: 21900944
Data indicate that the percentages of CD4(+)CXCR5(+) TFH in IA patients were positively correlated with AST. PMID: 21750724
liver enzymes, including AST, were significantly associated with IR according to direct clamp assessment, which were independent of the traditional risk factors in diabetic patients PMID: 20972737
Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
mildly elevated in severe acute respiratory syndrome patients during second week after onset of fever. PMID: 15306699
saliva AST and ALP may have roles in development of periodontal diseases PMID: 16681433
PTLP activity is positively associated with serum ALT1, GOT1 and GOT2 in type 2 diabetes mellitus and metabolic syndrome. PMID: 17877759
Abnormal serum aminotransferase values are uncommon in severely obese children in France. PMID: 17907318
Data revealed higher aspartate aminotransferase for women with a family history of diabetes, when adjusted for age and BMI. PMID: 18242760
Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
Neither ALT nor GGT concentrations were correlated with ALP concentration, but AST concentration was moderately correlated with ALP concentration. PMID: 19012630
Results suggest that alanine and aspartate aminotransferase levels and AST/ALT ratio do not seem to be reliable predictors for non-alcoholic steatohepatitis. PMID: 19370784
the management of patients with pyelonephritis should take into account that moderate and self-limited abnormalities in aminotransferase levels are frequent during the acute phase of the disease PMID: 19393479

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Class-I pyridoxal-phosphate-dependent aminotransferase family

Database Links

HGNC: 4432

OMIM: 138180

KEGG: hsa:2805

STRING: 9606.ENSP00000359539

UniGene: Hs.500756

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Code	CSB-E09603h
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Human Aspartate aminotransferase,AST ELISA Kit

Product Details

Citations

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Target Background