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| Code | CSB-E09603h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-94 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 97-110 | |||||
| The recovery of human AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
The human AST ELISA Kit is intended to measure human AST in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human AST concentration. The intensity of the colored product is directly proportional to the concentration of AST present in the sample. This human AST ELISA kit has high specificity and excellent sensitivity.
AST (GOT1) is an enzyme involved in the malate-aspartate (MA) shuttle that produces cytosolic glutamate by transferring amino residue from aspartate to α-ketoglutarate. It is important for the metabolism of amino acids, Krebs cycle activity, maintenance of NAD+/NADH ratio in cells, generation of purine/pyrimidine bases, urea and protein synthesis, and gluconeogenesis. An increased AST activity may be a sign of tissue damage, plasma membrane bleb formation, enhanced tissue expression, and macroenzymes. Elevated AST levels are commonly associated with inflammatory liver disease, alcoholic liver disease, cirrhosis, cholestatic syndromes, drug toxicity, acute myocardial infarction, septic shock, and skeletal muscle injury/trauma.
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