GOT1 Antibody

Code CSB-PA009679HA01HU
Size US$166
  • IHC image of CSB-PA009679HA01HU diluted at 1:250 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA009679HA01HU diluted at 1:250 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Mouse liver tissue, Mouse skeletal muscle tissue
    All lanes: GOT1 antibody at 4.5µg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 47, 45 kDa
    Observed band size: 47 kDa

  • Immunofluorescence staining of HepG2 cells with CSB-PA009679HA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GOT1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
AATC_HUMAN antibody; Aspartate aminotransferase 1 antibody; Aspartate aminotransferase antibody; Aspartate aminotransferase cytoplasmic antibody; Aspartate aminotransferase cytosolic antibody; ASTQTL1 antibody; cAspAT antibody; cCAT antibody; cysteine aminotransferase; cytoplasmic antibody; cysteine transaminase; cytoplasmic antibody; cytoplasmic antibody; ec antibody; GIG 18 antibody; GIG18 antibody; Glutamate oxaloacetate transaminase 1 antibody; Glutamate oxaloacetate transaminase soluble antibody; Glutamic oxaloacetic transaminase 1 soluble antibody; GOT 1 antibody; GOT1 antibody; Growth inhibiting protein 18 antibody; SGOT antibody; Transaminase A antibody
Raised in
Species Reactivity
Human, Mouse
Recombinant Human Aspartate aminotransferase, cytoplasmic protein (2-411AA)
Immunogen Species
Homo sapiens (Human)

The GOT1 Antibody (Product code: CSB-PA009679HA01HU) is Non-conjugated. For GOT1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA009679HB01HU GOT1 Antibody, HRP conjugated ELISA
FITC CSB-PA009679HC01HU GOT1 Antibody, FITC conjugated
Biotin CSB-PA009679HD01HU GOT1 Antibody, Biotin conjugated ELISA
Purification Method
>95%, Protein G purified
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Tested Applications
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Gene References into Functions
  1. high transaminase elevations do not predict severe complications or reflect remnant ischemic area. PMID: 29553104
  2. Missense variant (p.Gln208Glu, rs374966349) in glutamate oxaloacetate transaminase 1 (GOT1) was found, as a putative causal variant predisposing to Familial Macro-aspartate aminotransferase . PMID: 28716744
  3. HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway and GOT1 expression in human pancreatic ductal adenocarcinoma. PMID: 28544376
  4. ALT is a more suitable index than AST for developing a regression model. PMID: 28628604
  5. High AST1 expression is associated with high mortality in hemodialysis patients. PMID: 26333544
  6. GOT1 plays an important role in energy metabolism and ROS balance in chronic acidosis stress in pancreatic tumor cells. PMID: 28455244
  7. Early abnormal liver enzyme levels of aspartate aminotransferase and alanine aminotransferase may increase the prevalence of human cytomegalovirus antigenemia after hematopoietic stem cell transplantation. PMID: 28415934
  8. Elevated preoperative aspartate aminotransferase-to-platelet ratio index may be independently associated with poor overall survival and disease-free survival in hepatocellular carcinoma patients following curative resection PMID: 28351330
  9. Elevated levels of ALT are Associated with Cardiovascular disease. PMID: 27872510
  10. Elevated aspartate aminotransferase level was associated with hepatocellular carcinoma. PMID: 26881519
  11. The serum hepcidin, ferritin, alanine transaminase, aspartate transaminase, gamma-glutamyltransferase and bilirubin were examined in two independent Chinese cohorts consisted of 3455 individuals. PMID: 26290281
  12. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GOT1 rises due to up-regulation of hepatic expression of GOT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
  13. Human GOT1 was expressed in E coli, purified by affinity chromatography and preliminary crystallographic studies were done. PMID: 26003525
  14. We propose the GOT1 expression status as a simple and reliable prognostic biomarker in pancreatic ductal adenocarcinoma PMID: 25595905
  15. Significantly higher GOT levels were found in the 1-20 year age group of type 1 PMID: 24849491
  16. The aim of this study was to establish the reference intervals (RIs) of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate transaminase (AST), and creatinine (CREA) for apparently healthy elderly (Han ethnicity) in Shuyang, China. PMID: 25272068
  17. APRI and AST/ALT are independent predictors for significant fibrosis in chronic hepatitis C patients. PMID: 22352690
  18. This study highlights the necessity of intensive follow-up for those with elevated AST and ALT levels and comorbid alcohol use disorder for preventing excessive natural deaths. PMID: 22242166
  19. we discovered a rare in-frame deletion in GOT1 gene, which inactivates cAST enzyme in the Old Order Amish. PMID: 21900944
  20. Data indicate that the percentages of CD4(+)CXCR5(+) TFH in IA patients were positively correlated with AST. PMID: 21750724
  21. liver enzymes, including AST, were significantly associated with IR according to direct clamp assessment, which were independent of the traditional risk factors in diabetic patients PMID: 20972737
  22. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
  23. mildly elevated in severe acute respiratory syndrome patients during second week after onset of fever. PMID: 15306699
  24. saliva AST and ALP may have roles in development of periodontal diseases PMID: 16681433
  25. PTLP activity is positively associated with serum ALT1, GOT1 and GOT2 in type 2 diabetes mellitus and metabolic syndrome. PMID: 17877759
  26. Abnormal serum aminotransferase values are uncommon in severely obese children in France. PMID: 17907318
  27. Data revealed higher aspartate aminotransferase for women with a family history of diabetes, when adjusted for age and BMI. PMID: 18242760
  28. Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
  29. Neither ALT nor GGT concentrations were correlated with ALP concentration, but AST concentration was moderately correlated with ALP concentration. PMID: 19012630
  30. Results suggest that alanine and aspartate aminotransferase levels and AST/ALT ratio do not seem to be reliable predictors for non-alcoholic steatohepatitis. PMID: 19370784
  31. the management of patients with pyelonephritis should take into account that moderate and self-limited abnormalities in aminotransferase levels are frequent during the acute phase of the disease PMID: 19393479

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Subcellular Location
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family
Database Links

HGNC: 4432

OMIM: 138180

KEGG: hsa:2805

STRING: 9606.ENSP00000359539

UniGene: Hs.500756

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