Human CD2-associated protein(CD2AP) ELISA kit

Code CSB-EL004914HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CD2AP ELISA Kit was designed for the quantitative measurement of Human CD2AP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

Target Name CD2-associated protein
Alternative Names Adapter protein CMS ELISA Kit; AL024079 ELISA Kit; C78928 ELISA Kit; Cas ligand with multiple SH3 domains ELISA Kit; CD2 associated protein ELISA Kit; CD2-associated protein ELISA Kit; CD2AP ELISA Kit; CD2AP_HUMAN ELISA Kit; CMS ELISA Kit; Mesenchyme to epithelium transition protein with SH3 domains 1 ELISA Kit; METS 1 ELISA Kit; Mets1 ELISA Kit
Abbreviation CD2AP
Uniprot No. Q9Y5K6
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD2AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 82-93
1:2 Average % 97
Range % 91-103
1:4 Average % 92
Range % 87-98
1:8 Average % 101
Range % 96-105
Recovery
The recovery of human CD2AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 82-94
EDTA plasma (n=4) 93 86-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1200 2.864 2.755 2.810 2.694
600 2.137 2.212 2.175 2.059
300 1.456 1.537 1.497 1.381
150 0.956 0.946 0.951 0.835
75 0.497 0.489 0.493 0.377
37.5 0.274 0.289 0.282 0.166
18.75 0.218 0.210 0.214 0.098
0 0.116 0.115 0.116  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. Plays a role in epithelial cell junctions formation.
Gene References into Functions
  1. CD2AP is an sporadic-Alzheimer's-disease risk gene in a southern Chinese Han population. PMID: 28392172
  2. data suggest that CD2AP acts as a negative regulator of ICAM-1 clustering, which limits the formation of ICAM-1 adhesion complexes to prevent uncontrolled neutrophil adhesion and transcellular transmigration. PMID: 28484055
  3. CD2AP expression in renal tubules may histologically associate with tissue hypoxia and reflected recovery from CsA-mediated renal injury in nephrotic syndrome patients. PMID: 26975192
  4. Silencing Cindr in nephrocytes led to dramatic nephrocyte functional impairment and shortened life span, as well as collapse of nephrocyte lacunar channels and effacement of nephrocyte slit diaphragms. These phenotypes could be rescued by expression of a wild-type human CD2AP gene, but not a mutant allele derived from a patient with CD2AP-associated NS. PMID: 28164240
  5. Study found a novel association of CD2AP with plasma homocysteine in participants with African ancestry and found a new variant in the candidate gene CBS associated with homocysteine PMID: 26519441
  6. CD2AP rs9349407 polymorphism contributes to Alzheimer's disease susceptibility. PMID: 25092125
  7. discovered novel interaction candidates for CD2AP and characterized subtle yet significant differences in the recognition preferences of its three SH3 domains for c-CBL, ALIX, and RIN3 PMID: 26296892
  8. we present the first demonstration that the purified SH3 domains of the CD2AP/Cin85 protein family are able to directly bind the p53 protein, and to discriminate between the two polymorphic variants P72R PMID: 25261582
  9. CD2-Associated Protein affects Abeta levels and Abeta42/Abeta40 ratio in vitro PMID: 25887956
  10. CD2AP gene variants may contribute to susceptibility to end-stage renal disease in patients with type 1 diabetes. PMID: 23681557
  11. FSGS3/CD2AP has a role of barbed-end capping in junctional actin dynamics. PMID: 24322428
  12. E2F1 up-regulates the human CD2AP promoter. PMID: 22880102
  13. CD2AP is highly expressed in human plasmacytoid dendritic cells (DC) and positively regulates blood DC antigen 2 (BDCA2)/Fc fragment of IgE high affinity I receptor (FcepsilonR1gamma) signaling. PMID: 22706086
  14. identifies CD2AP as the gatekeeper of the podocyte TGF-beta response through its regulation of CatL expression and defines a molecular mechanism underlying proteinuric kidney disease PMID: 21911934
  15. found that the N-terminal SH3 domain of both adaptor proteins CD2AP and CIN85 are the most stable SH3 domains that have been studied until now PMID: 21519904
  16. found independent evidence for association for Alzheimer's disease susceptibility loci at EPHA1, CD33 and CD2AP PMID: 21460840
  17. Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA1 are associated with late-onset Alzheimer's disease. PMID: 21460841
  18. Coexpression of CIN85/Ruk(L) with CD2AP led to a decreased binding of CIN85/Ruk(L) to nephrin and podocin, which indicates a functional competition between CD2AP and CIN85/Ruk(L). PMID: 20457601
  19. Data identify CD2AP as a novel Rac1-associated adapter protein that participates in the regulation of epithelial cell-cell contact. PMID: 20404345
  20. The authors report that CD2AP, an endocytosis-associated and cortactin-binding protein, is a novel and important component of enteropathogenic Escherichia coli pedestal formation that also utilizes Y474 phosphorylation of the bacterial Tir. PMID: 20515931
  21. The absence of mutations of CD2AP in this study suggests that there are other genetic causes of steroid-resistant nephrotic syndrome PMID: 19956976
  22. CD2AP, through facilitating conjugate formation and directed transport of lytic granules, plays an important role in NK cells killing. PMID: 19945749
  23. PSTPIP1 acts downstream of CD2/CD2AP to link CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. PMID: 12530983
  24. Cd2 antigen is linked to CAPZ via this protein and CIN85 PMID: 12690097
  25. two patients with focal segmental glomerulosclerosis had a mutation predicted to ablate expression of one CD2AP allele, implicating CD2AP as a determinant of human susceptibility to glomerular disease PMID: 12764198
  26. exposure to normal and non-nephrotic human plasma leads to a concentration of nephrin, podocin, CD2AP, and actin at the cell surface in podocytes PMID: 15659563
  27. CD2AP is involved in cytokinesis. PMID: 15800069
  28. CD2AP has a role in the regulation of the actin cytoskeleton PMID: 16707503
  29. CFBP is a novel tyrosine-phosphorylated protein that might function as a regulator of CIN85/CD2AP PMID: 16895919
  30. structures support the notion that, despite clear differences in the interaction surface, both Cbl-b and CD2 can mediate multimerization of N-terminal CMS SH3 domains PMID: 17020880
  31. This work indicates the solution structure of CMS_SH3_B bears the canonical beta-beta-beta-beta-alpha-beta fold and a new binding site in c-Cbl involved in its interaction with CMS, which probably contributes to the clustering of CMS. PMID: 17188587
  32. CIN85 is expressed as multiple isoforms that share the coiled-coil domain, suggesting that heterotypic interactions with CMS provides a mechanism to regulate CMS binding to F-actin and thus for modulating dynamic rearrangements of the cytoskeleton. PMID: 17606992
  33. Focal segmental glomerulosclerosis in a patient homozygous for a CD2AP mutation. PMID: 17713465
  34. identified a polyproline-arginine sequence in the pTalpha cytoplasmic tail that interacted in vitro with SH3 domains of the CIN85/CMS family of adaptors, and mediated the recruitment of multiprotein complexes involving all (CMS, CIN85, and CD2BP3) members PMID: 17823309
  35. The three-dimensional structure of CD2AP SH3-C contains all the features that are typically found in other SH3 domains, including the general binding site for the recognition of polyproline sequences. PMID: 17922258
  36. promotor activity in rental tubular epithelial cells is regulated by CREB and Sp1 PMID: 18396147
  37. Sp1/Sp3 binding sites play a critical role in the CD2AP regulation. PMID: 18791326
  38. CD2AP mutations modify the interaction with CD2 in lymphocytes and alter the composition of the renal slit diaphragm. PMID: 19131354

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Involvement in disease Focal segmental glomerulosclerosis 3 (FSGS3)
Subcellular Location Cytoplasm, cytoskeleton. Cell projection, ruffle. Cell junction.
Tissue Specificity Widely expressed in fetal and adult tissues.
Database Links

HGNC: 14258

OMIM: 604241

KEGG: hsa:23607

STRING: 9606.ENSP00000352264

UniGene: Hs.485518

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