Name: CD2AP Antibody
Brand: CUSABIO
SKU: CSB-PA004914LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CD2AP Polyclonal antibody

Uniprot No.

Q9Y5K6

Target Names

CD2AP

Alternative Names

Adapter protein CMS antibody; AL024079 antibody; C78928 antibody; Cas ligand with multiple SH3 domains antibody; CD2 associated protein antibody; CD2-associated protein antibody; CD2AP antibody; CD2AP_HUMAN antibody; CMS antibody; Mesenchyme to epithelium transition protein with SH3 domains 1 antibody; METS 1 antibody; Mets1 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human CD2-associated protein (333-426AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CD2AP Antibody (Product code: CSB-PA004914LA01HU) is Non-conjugated. For CD2AP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA004914LB01HU	CD2AP Antibody, HRP conjugated	ELISA
FITC	CSB-PA004914LC01HU	CD2AP Antibody, FITC conjugated
Biotin	CSB-PA004914LD01HU	CD2AP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. Plays a role in epithelial cell junctions formation.

Gene References into Functions

CD2AP is an sporadic-Alzheimer's-disease risk gene in a southern Chinese Han population. PMID: 28392172
data suggest that CD2AP acts as a negative regulator of ICAM-1 clustering, which limits the formation of ICAM-1 adhesion complexes to prevent uncontrolled neutrophil adhesion and transcellular transmigration. PMID: 28484055
CD2AP expression in renal tubules may histologically associate with tissue hypoxia and reflected recovery from CsA-mediated renal injury in nephrotic syndrome patients. PMID: 26975192
Silencing Cindr in nephrocytes led to dramatic nephrocyte functional impairment and shortened life span, as well as collapse of nephrocyte lacunar channels and effacement of nephrocyte slit diaphragms. These phenotypes could be rescued by expression of a wild-type human CD2AP gene, but not a mutant allele derived from a patient with CD2AP-associated NS. PMID: 28164240
Study found a novel association of CD2AP with plasma homocysteine in participants with African ancestry and found a new variant in the candidate gene CBS associated with homocysteine PMID: 26519441
CD2AP rs9349407 polymorphism contributes to Alzheimer's disease susceptibility. PMID: 25092125
discovered novel interaction candidates for CD2AP and characterized subtle yet significant differences in the recognition preferences of its three SH3 domains for c-CBL, ALIX, and RIN3 PMID: 26296892
we present the first demonstration that the purified SH3 domains of the CD2AP/Cin85 protein family are able to directly bind the p53 protein, and to discriminate between the two polymorphic variants P72R PMID: 25261582
CD2-Associated Protein affects Abeta levels and Abeta42/Abeta40 ratio in vitro PMID: 25887956
CD2AP gene variants may contribute to susceptibility to end-stage renal disease in patients with type 1 diabetes. PMID: 23681557
FSGS3/CD2AP has a role of barbed-end capping in junctional actin dynamics. PMID: 24322428
E2F1 up-regulates the human CD2AP promoter. PMID: 22880102
CD2AP is highly expressed in human plasmacytoid dendritic cells (DC) and positively regulates blood DC antigen 2 (BDCA2)/Fc fragment of IgE high affinity I receptor (FcepsilonR1gamma) signaling. PMID: 22706086
identifies CD2AP as the gatekeeper of the podocyte TGF-beta response through its regulation of CatL expression and defines a molecular mechanism underlying proteinuric kidney disease PMID: 21911934
found that the N-terminal SH3 domain of both adaptor proteins CD2AP and CIN85 are the most stable SH3 domains that have been studied until now PMID: 21519904
found independent evidence for association for Alzheimer's disease susceptibility loci at EPHA1, CD33 and CD2AP PMID: 21460840
Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA1 are associated with late-onset Alzheimer's disease. PMID: 21460841
Coexpression of CIN85/Ruk(L) with CD2AP led to a decreased binding of CIN85/Ruk(L) to nephrin and podocin, which indicates a functional competition between CD2AP and CIN85/Ruk(L). PMID: 20457601
Data identify CD2AP as a novel Rac1-associated adapter protein that participates in the regulation of epithelial cell-cell contact. PMID: 20404345
The authors report that CD2AP, an endocytosis-associated and cortactin-binding protein, is a novel and important component of enteropathogenic Escherichia coli pedestal formation that also utilizes Y474 phosphorylation of the bacterial Tir. PMID: 20515931
The absence of mutations of CD2AP in this study suggests that there are other genetic causes of steroid-resistant nephrotic syndrome PMID: 19956976
CD2AP, through facilitating conjugate formation and directed transport of lytic granules, plays an important role in NK cells killing. PMID: 19945749
PSTPIP1 acts downstream of CD2/CD2AP to link CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. PMID: 12530983
Cd2 antigen is linked to CAPZ via this protein and CIN85 PMID: 12690097
two patients with focal segmental glomerulosclerosis had a mutation predicted to ablate expression of one CD2AP allele, implicating CD2AP as a determinant of human susceptibility to glomerular disease PMID: 12764198
exposure to normal and non-nephrotic human plasma leads to a concentration of nephrin, podocin, CD2AP, and actin at the cell surface in podocytes PMID: 15659563
CD2AP is involved in cytokinesis. PMID: 15800069
CD2AP has a role in the regulation of the actin cytoskeleton PMID: 16707503
CFBP is a novel tyrosine-phosphorylated protein that might function as a regulator of CIN85/CD2AP PMID: 16895919
structures support the notion that, despite clear differences in the interaction surface, both Cbl-b and CD2 can mediate multimerization of N-terminal CMS SH3 domains PMID: 17020880
This work indicates the solution structure of CMS_SH3_B bears the canonical beta-beta-beta-beta-alpha-beta fold and a new binding site in c-Cbl involved in its interaction with CMS, which probably contributes to the clustering of CMS. PMID: 17188587
CIN85 is expressed as multiple isoforms that share the coiled-coil domain, suggesting that heterotypic interactions with CMS provides a mechanism to regulate CMS binding to F-actin and thus for modulating dynamic rearrangements of the cytoskeleton. PMID: 17606992
Focal segmental glomerulosclerosis in a patient homozygous for a CD2AP mutation. PMID: 17713465
identified a polyproline-arginine sequence in the pTalpha cytoplasmic tail that interacted in vitro with SH3 domains of the CIN85/CMS family of adaptors, and mediated the recruitment of multiprotein complexes involving all (CMS, CIN85, and CD2BP3) members PMID: 17823309
The three-dimensional structure of CD2AP SH3-C contains all the features that are typically found in other SH3 domains, including the general binding site for the recognition of polyproline sequences. PMID: 17922258
promotor activity in rental tubular epithelial cells is regulated by CREB and Sp1 PMID: 18396147
Sp1/Sp3 binding sites play a critical role in the CD2AP regulation. PMID: 18791326
CD2AP mutations modify the interaction with CD2 in lymphocytes and alter the composition of the renal slit diaphragm. PMID: 19131354

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Involvement in disease

Focal segmental glomerulosclerosis 3 (FSGS3)

Subcellular Location

Cytoplasm, cytoskeleton. Cell projection, ruffle. Cell junction.

Tissue Specificity

Widely expressed in fetal and adult tissues.

Database Links

HGNC: 14258

OMIM: 604241

KEGG: hsa:23607

STRING: 9606.ENSP00000352264

UniGene: Hs.485518

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Code	CSB-PA004914LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat kidney tissue All lanes: CD2AP antibody at 11μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa IHC image of CSB-PA004914LA01HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA004914LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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CD2AP Antibody

Product Details

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Target Background