Human Collagen alpha-3(VI) chain (COL6A3) ELISA kit

Instructions
Code CSB-EL005753HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name collagen, type VI, alpha 3
Alternative Names DKFZp686D23123, DKFZp686K04147, DKFZp686N0262, FLJ34702, FLJ98399, OTTHUMP00000202895|alpha 3 type 6 collagen|collagen 6, alpha-3 polypeptide|collagen alpha-3(6) chain,Collagen alpha-3(VI) chain (COL6A3)
Abbreviation COL6A3
Uniprot No. P12111
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human COL6A3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 86    
Range % 80-89    
1:2 Average % 91    
Range % 87-95    
1:4 Average % 92    
Range % 87-98    
1:8 Average % 93    
Range % 86-97    
Recovery
The recovery of human COL6A3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 87 82-91    
EDTA plasma (n=4) 102 97-106    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
100 1.988 1.872 1.930 1.821    
50 1.192 1.262 1.227 1.118    
25 0.724 0.769 0.747 0.638    
12.5 0.451 0.467 0.459 0.350    
6.25 0.304 0.314 0.309 0.200    
3.12 0.217 0.222 0.220 0.111    
1.56 0.160 0.169 0.165 0.056    
0 0.108 0.109 0.109      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Collagen VI acts as a cell-binding protein.
Involvement in disease Bethlem myopathy 1 (BTHLM1); Ullrich congenital muscular dystrophy 1 (UCMD1); Dystonia 27 (DYT27)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Type VI collagen family
Database Links

HGNC: 2213

OMIM: 120250

KEGG: hsa:1293

STRING: 9606.ENSP00000295550

UniGene: Hs.233240

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