Human Complement C1s subcomponent(C1S) ELISA kit

Instructions
Code CSB-EL003657HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name complement component 1, s subcomponent
Alternative Names Basic proline rich peptide IB 1 ELISA Kit; C1 esterase ELISA Kit; C1S ELISA Kit; C1S_HUMAN ELISA Kit; Complement C1s subcomponent ELISA Kit; Complement C1s subcomponent heavy chain ELISA Kit; Complement C1s subcomponent light chain ELISA Kit; Complement component 1 s subcomponent ELISA Kit; Complement component 1 subcomponent s ELISA Kit; FLJ44757 ELISA Kit
Abbreviation C1S
Uniprot No. P09871
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 ng/mL-1600 ng/mL
Sensitivity 6.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C1S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 100
Range % 91-104
1:200 Average % 107
Range % 98-110
1:400 Average % 88
Range % 82-93
1:800 Average % 96
Range % 87-99
Recovery
The recovery of human C1S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 110 98-114
EDTA plasma (n=4) 98 90-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1600 2.740 2.685 2.713 2.598
800 2.346 2.276 2.311 2.196
400 1.488 1.454 1.471 1.356
200 0.756 0.768 0.762 0.647
100 0.396 0.411 0.404 0.289
50 0.246 0.239 0.243 0.128
25 0.162 0.170 0.166 0.051
0 0.113 0.117 0.115  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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 Q&A
Q:

***Please let us know the active ingredient of the stop solution and its
concentration***
***Please let us know the type, concentration, and state
(liquid/lyophilized) of the preservatives used*** ***Please let us know the
expression host of the standard***

A:
Thanks for your inquiry!
Pls check our information:
CSB-EL003657HU
Stop Solution is 4N sulfuric acid
Preservatives is liquid porclin 300(1:3000);
Standard is 293 cell-derived recombinant protein.

Target Data

Function C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
Gene References into Functions
  1. TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins. PMID: 24695853
  2. Data indicate that complement C1s mRNA level was low in ICR-derived glomerulonephritis (ICGN) mice liver as compared with age-matched ICR mice. PMID: 23989031
  3. A molecular switch governs the interaction between the human complement protease C1s and its substrate, complement C4. PMID: 23592783
  4. Four positively charged amino acids on the serine protease domain appear to form a catalytic exosite that is required for efficient cleavage of C4 in the classical pathway of complement. PMID: 22855709
  5. Detailed mapping of post-translational modifications and insights into the C1r/C1s binding sites. PMID: 20008834
  6. Interaction with the prime side residues at the cleavage point in C1s enhances the affinity of the enzyme for complement 2 and complement 4 substrates; these prime subsite residues mediate positive cooperativity in the cleavage of the substrate. PMID: 14674770
  7. full specificity of the enzyme using a randomized phage display library PMID: 16169853
  8. There are splice variants of C1s mRNA transcripts in normal human cells PMID: 18062908

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Involvement in disease Complement component C1s deficiency (C1SD); Ehlers-Danlos syndrome, periodontal type, 2 (EDSPD2)
Protein Families Peptidase S1 family
Database Links

HGNC: 1247

OMIM: 120580

KEGG: hsa:716

STRING: 9606.ENSP00000328173

UniGene: Hs.458355

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