Human Ferritin, Heavy Polypeptide (FTH) ELISA Kit

Instructions
Code CSB-E16932h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ferritin, heavy polypeptide 1
Alternative Names Apoferritin ELISA Kit; Cell proliferation inducing gene 15 protein ELISA Kit; Cell proliferation-inducing gene 15 protein ELISA Kit; F HC ELISA Kit; Ferritin H subunit ELISA Kit; Ferritin heavy chain ELISA Kit; Ferritin heavy polypeptide 1 ELISA Kit; FHC ELISA Kit; FRIH ELISA Kit; FRIH_HUMAN ELISA Kit; FTH 1 ELISA Kit; FTH ELISA Kit; FTH1 ELISA Kit; FTH1 protein ELISA Kit; FTHL 6 ELISA Kit; FTHL6 ELISA Kit; Iron overload autosomal dominant ELISA Kit; MGC104426 ELISA Kit; N-terminally processed ELISA Kit; OK/SW-cl.84 ELISA Kit; PIG 15 ELISA Kit; PIG15 ELISA Kit; Placenta immunoregulatory factor ELISA Kit; PLIF ELISA Kit; Proliferation inducing gene 15 protein ELISA Kit; Proliferation inducing protein 15 ELISA Kit
Abbreviation FTH1
Uniprot No. P02794
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.45 ng/mL-30 ng/mL
Sensitivity 0.11 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 85-103  
1:2 Average % 90  
Range % 87-106  
1:4 Average % 92  
Range % 85-100  
1:8 Average % 95  
Range % 89-105  
Recovery
The recovery of human FTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 83-105  
EDTA plasma (n=4) 96 88-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
30 2.712 2.720 2.716 2.594  
15 1.919 1.851 1.885 1.763  
7.5 1.166 1.124 1.145 1.023  
3.75 0.725 0.737 0.731 0.609  
1.8 0.406 0.428 0.417 0.295  
0.9 0.282 0.301 0.292 0.170  
0.45 0.193 0.202 0.198 0.076  
0 0.120 0.123 0.122    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
Gene References into Functions
  1. Human oligodendrocytes undergo apoptosis when exposed to Sema4A and take up H-ferritin for meeting iron requirements and that these functions are mediated via the Tim-1 receptor. H-ferritin can block Sema4A-mediated cytotoxicity. Sema4A is detectable in the CSF of multiple sclerosis patients and HIV-seropositive persons and can induce oligodendrocyte cell death. PMID: 29457657
  2. High serum ferritin (SF) and the overall survival of allogeneic hematopoietic SF was only significantly associated with prolonged RBC transfusion dependence post-transplantation. PMID: 29046127
  3. the role of FTH1 in the FIH control of HIF-1 activity, is reported. PMID: 29580991
  4. Study demonstrate that the amounts of ferritin heavy subunit are inversely correlated, in K562 cells, to H19/miR-675 levels, and that this phenomenon may be largely attributed to the increase in ROS production induced by the FHC silencing. PMID: 29544765
  5. Findings indicate that induction of EMT, increased migration and survival depend, in MCF-7 and H460 cells, on the release of FHC control on two pathways, namely the iron/ROS metabolism and CXCR4/CXCL12 axis. PMID: 28774348
  6. ferritin heavy chain has a role in ovarian cancer stem cell expansion and epithelial to mesenchymal transition PMID: 27566559
  7. Report in vivo magnetic resonance imaging of xenografted neuroblastoma tumors using FTH1 reporter gene expression controlled by a tet-on switch. PMID: 27732930
  8. The H-ferritin could bind up to 24 NCOA4(383-522) fragments forming highly stable and insoluble complexes. The binding was partially inhibited only by Fe(II) among the various divalent metal ions analyzed. The iron-dependent, highly-specific formation of the remarkably stable H-ferritin-NCOA4 complex shown in this work may be important for the characterization of the mechanism of ferritinophagy. PMID: 28754384
  9. Recombinant human H-chain ferritin nanocages were characterized by transmission electron microscopy and dynamic light scattering. These results indicate that H-chain ferritin is able to self-assemble into nanocages with a narrow size distribution. PMID: 26853188
  10. Among myelodysplastic syndromes patients only, CD163 + macrophage density and HO1 and H-ferritin expression by CD163 + macrophages increased in tandem with marrow iron. High HO1 was significantly associated with shorter overall survival. PMID: 26758041
  11. Low levels of FTH-positive tumor cells and microglia/macrophages were associated with poor survival in anaplastic astrocytomas, while high amounts of FTL-positive microglia/macrophages had a negative prognostic value PMID: 28837569
  12. there is no significant relationship between serum ferritin concentrations and depressive symptoms among Chinese adults. PMID: 27611581
  13. Data suggest that, in homopolymeric H-subunit ferritin (HuHF), iron oxidation proceeds with a 2:1 Fe(II):O(2) stoichiometry at iron level of 2 Fe(II) atoms/H-subunit with generation of H2O2; L-subunit-rich heteropolymeric ferritin also facilitates iron oxidation at the ferroxidase center and additionally promotes oxidation at the mineral surface once iron-binding capacity has been exceeded. PMID: 28636371
  14. Elevated serum ferritin levels are associated with hematologic malignancies. PMID: 25124580
  15. Ferritin light chain and ferritin heavy chain are required for the neural differentiation of bone marrow-derived mesenchymal stem cells under extremely low-frequency electromagnetic field. PMID: 26602884
  16. Human FTH1 is a general pro-survival sequence. PMID: 26886577
  17. H-ferritin tissue expression and the number of CD68+/H-ferritin+ cells were increased in the lymph nodes of adult-onset Still's disease patients, and these results correlated significantly with disease severity PMID: 26540556
  18. Stopped-flow kinetics assays revealed that human H ferritin has different levels of activity compared with its R. catesbeiana counterpart PMID: 26327381
  19. Studies indicate that the the best characterized cytosolic ferritins in mammals are encoded by two genes, FTH and FTL, with four exons and similar structures. PMID: 26518749
  20. high cytoplasmic FTH1 was associated with favorable prognosis whereas nuclear FTH1 staining was associated with adverse prognosis in triple negative breast cancer PMID: 24742827
  21. The goals of this study were to evaluate the multilineage differentiation ability of human mesenchymal stem cells expressing an MRI reporter, human ferritin heavy chain (FTH). PMID: 25448225
  22. Data suggest that iron-independent effects of ferritin in myeloma should be prospectively investigated in preclinical and clinical studies. PMID: 24512320
  23. Significant differences were seen in the levels of serum iron and hepcidin but not ferritin between the -582A>G hepcidin polymorphism genotypes. PMID: 25134646
  24. High ferritin expression is associated with malignant phenotype of breast cancer. PMID: 25265351
  25. High serum ferritin levels are associated with pancreatic cancer. PMID: 24446242
  26. FTH provides metabolic adaptation to tissue Fe overload. [Review] PMID: 24124891
  27. Tumor necrosis factor-alpha attenuates starvation-induced apoptosis through upregulation of ferritin heavy chain in hepatocellular carcinoma cells. PMID: 24066693
  28. Elevated serum ferritin level is associated with the incident type 2 diabetes in healthy Korean men. PMID: 24098686
  29. Children with autism spectrum disorders have significantly lower ferritin levels compared with controls and higher levels of sleep fragmentation and periodic limb movements. PMID: 24053984
  30. Data indicate that serum highest TNF-alpha and IL-6 and higher ferritin are among those with intense steatosis. PMID: 23892696
  31. Traumatic brain injury upregulates ferritin-heavy-chain in pericontusional cortex. PMID: 23079850
  32. Increased levels of serum ferritin are adversely associated with cardiac function following myocardial infarction treated with percutaneous coronary intervention. PMID: 23079089
  33. In normal bone marrow, ferritin H antibody stained early erythroid precursors and macrophages. In leukemic bone marrow, ferritin H was selectively expressed in erythroid blasts (M6), whereas all other blasts were negative. PMID: 23611361
  34. The present study provides information about the metal binding sites of human H-chain ferritin and the pathway of ferrous ions (Fe2+) from the cytoplasm to the interior of the ferritin nanocage. PMID: 23344859
  35. Serum ferritin levels in infectious diseases was lower than those in hematologic diseases. PMID: 23324584
  36. Data indicate that ferritin levels and hyperuricemia are two independently significant factors associated with alanine aminotransferase (ALT) elevation among obese adolescents. PMID: 23119080
  37. Increased levels of ferritin and decreased total antioxidant status indicated increasing inflammation in chronic hepatitis C. PMID: 22760008
  38. H-ferritin overexpression promotes radiation-induced leukemia/lymphoma. PMID: 22843505
  39. The hepcidin:ferritin ratio is reduced in relation to increasing fibrosis in chronic liver disease. PMID: 22676252
  40. To study whether the human ferritin heavy chain (FTH1) can be expressed in Hansenula polymorpha, we integrated an expression cassette for FTH1, analyzed the protein expression, and also designed a FTH1-PTH fusion protein. PMID: 22212821
  41. Induction of ferritin heavy chain expression results in severe inhibition of apoB-100 secretion from hepatoma cells during HCV infection. PMID: 22443280
  42. Survivin inhibition by an interacting recombinant peptide, derived from the human ferritin heavy chain, impedes tumor cell growth PMID: 22426960
  43. Elevated serum ferrin in infants is associated with congenital hypothyroidism. PMID: 22768652
  44. Data provide useful insights into biomineralization of ferritin under in vivo fever conditions as well as in biomimetic synthesis of nanomaterials using ferritin. PMID: 22020807
  45. FTH1 can inhibit the activation of the JNK signaling pathway and can bind to Daxx, inhibiting Daxx-mediated apoptosis. PMID: 21573799
  46. H ferritin silenced human metastatic melanoma cells are characterized by a decreased growth activity, a reduced invasiveness, and a reduced cell adhesion capability. PMID: 22043922
  47. There was a positive correlation between reactive oxygen species levels and serum ferritin levels in myelodysplastic syndrome patients PMID: 22117997
  48. results showed the association of secreted IL-10, FHC and iron homoeostasis in Chlamydia trachomatis-infected HeLa-229 cells PMID: 21413929
  49. FTH1 is a functional target gene of the BACH1 transcription factor according to ChIP-seq and knockdown analysis in HEK 293 cells. PMID: 21555518
  50. Results illustrate how silencing H-ferritin can effectively sensitize tumors to chemotherapy. PMID: 21385903

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Involvement in disease Hemochromatosis 5 (HFE5)
Protein Families Ferritin family
Tissue Specificity Expressed in the liver.
Database Links

HGNC: 3976

OMIM: 134770

KEGG: hsa:2495

STRING: 9606.ENSP00000273550

UniGene: Hs.524910

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