Product Details

Uniprot No.

Target Names

Alternative Names

Apoferritin antibody; Cell proliferation inducing gene 15 protein antibody; Cell proliferation-inducing gene 15 protein antibody; F HC antibody; Ferritin H subunit antibody; Ferritin heavy chain antibody; Ferritin heavy polypeptide 1 antibody; FHC antibody; FRIH antibody; FRIH_HUMAN antibody; FTH 1 antibody; FTH antibody; FTH1 antibody; FTH1 protein antibody; FTHL 6 antibody; FTHL6 antibody; Iron overload autosomal dominant antibody; MGC104426 antibody; N-terminally processed antibody; OK/SW-cl.84 antibody; PIG 15 antibody; PIG15 antibody; Placenta immunoregulatory factor antibody; PLIF antibody; Proliferation inducing gene 15 protein antibody; Proliferation inducing protein 15 antibody

Species Reactivity

Human

Immunogen

Recombinant Human FTH1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

18C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Ferritin heavy chain (FTH1) serves as the catalytic subunit of the ferritin complex, possessing ferroxidase activity essential for converting reactive ferrous iron to the safer ferric form for intracellular storage. This iron-sequestering function positions FTH1 at the intersection of cellular metabolism, oxidative stress response, and proliferation control, making it a compelling target across neurodegenerative disease research, cancer biology, and cardiovascular studies where iron homeostasis plays a critical role.

This recombinant monoclonal antibody, clone 18C10, offers the reproducibility and consistency that demanding experimental workflows require. Because it derives from a defined sequence rather than traditional hybridoma methods, you can expect uniform performance across lots—particularly valuable for longitudinal studies or multi-site collaborations where antibody variability can introduce unwanted noise into your data.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms specific detection of FTH1 at the expected 22 kDa molecular weight in both U251 glioblastoma and JK (Jurkat) cell lysates, with effective working dilutions ranging from 1:1000 to 1:5000. Immunohistochemistry studies using paraffin-embedded human tissues—including normal liver and rectal cancer specimens—show reliable staining at 1:50 dilution following citrate buffer antigen retrieval. Flow cytometry validation in 293 cells demonstrates clear separation from isotype controls, confirming utility for intracellular protein quantification in fixed and permeabilized samples.

The unconjugated format and affinity-purified preparation provide flexibility for pairing with your preferred detection systems. Whether investigating iron metabolism in neurodegeneration, exploring FTH1's role in tumor microenvironments, or examining its involvement in cardiac pathophysiology, this antibody delivers the specificity and application range your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA009030MA1HU
Size	US$210
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Image	Western Blot Positive WB detected in: U251 whole cell lysate,JK whole cell lysate All lanes: FTH1 antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa IHC image of CSB-RA009030MA1HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA009030MA1HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 293 cells stained with CSB-RA009030MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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FTH1 Recombinant Monoclonal Antibody

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