Human Fibrinogen-like protein 1(FGL1) ELISA kit

Code CSB-EL008653HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Alternative Names
FGL 1 ELISA Kit; Fgl1 ELISA Kit; FGL1_HUMAN ELISA Kit; Fibrinogen like 1 ELISA Kit; Fibrinogen like protein 1 ELISA Kit; Fibrinogen related protein 1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Hepassocin ELISA Kit; Hepatocellular carcinoma related sequence ELISA Kit; Hepatocyte derived fibrinogen related protein 1 ELISA Kit; Hepatocyte-derived fibrinogen-related protein 1 ELISA Kit; HFREP 1 ELISA Kit; HFREP-1 ELISA Kit; HFREP1 ELISA Kit; HP 041 ELISA Kit; HP-041 ELISA Kit; HP041 ELISA Kit; LFIRE 1 ELISA Kit; LFIRE-1 ELISA Kit; LFIRE1 ELISA Kit; Liver fibrinogen related protein 1 ELISA Kit; Liver fibrinogen-related protein 1 ELISA Kit; MFIRE 1 ELISA Kit; MGC108569 ELISA Kit; MGC12455 ELISA Kit; MGC37822 ELISA Kit; OTTHUMP00000122468 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/ml-10 ng/ml.
0.039 ng/ml
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Materials provided
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human FGL1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled FGL1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

The Human Fibrinogen-like protein 1 (FGL1) ELISA Kit quantifies FGL1 in human serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for cardiovascular research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which FGL1 in the samples or standards are sandwiched between pre-coated FGL1 antibody and Biotin-conjugated FGL1 antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of FGL1 bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of FGL1 in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

FGL1, also known as FREP1 or hepassocin, is a novel hepatocyte belonging to the fibrinogen family. It plays an important role in hepatic steatosis, insulin resistance, liver regeneration, and liver protection. FGL1 is normally released by the liver in low levels but by cancer in high levels. FGL1 acts as a major ligand of LAG-3 (Lymphocyte-activation gene 3) that mediates T cell inhibition, so blockade of the FGL1-LAG-3 interaction enhances antitumor immunity.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here


We would like to use your FGL1 Elisa Kit CSB-EL008653HU. Unfortunately, our plasma samples are mixed with sodium citrate. Is it possible to use your product even though the instructions only mention EDTA or heparin?


In principle, if the reagents do not affect protein structure changes (including protein denaturation), the reagents can be used.
But too acid or alkali reagent will affect the protein. It is unclear on the specific pH of sodium acetate you mentioned.

Generally, we detect plasma directly by preparing it without using other reagents (such as Sodium acetate), so we can't judge whether it has any effect.
If your sample has not been prepared, it is recommended to follow the instruction with the kit.
If it has been prepared, please arrange a preliminary experiment to check the result.

Target Background

(From Uniprot)
Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes.
Gene References into Functions
  1. FGL1 promotes invasion and metastasis of gastric cancer and is associated with poor prognosis. PMID: 29845203
  2. findings highlight the crucial role of HFREP1 in insulin resistance and diabetes, and provide a potential strategy and biomarker for developing therapeutic approaches to combat these diseases. PMID: 27221093
  3. Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
  4. Here, the application of small ubiquitin-related modifier (SUMO) fusion technology in combination with four different chaperone teams on the soluble expression of recombinant HPS protein was explored and analyzed. PMID: 24084006
  5. Findings suggest that hepassocin promotes hepatic cell line L02 cells proliferation via an autocrine mechanism and inhibits HCC cells proliferation by an intracrine pathway. PMID: 21618590
  6. data demonstrated that liver-specific gene LFIRE-1/HFREP-1 was frequently downregulated and might possess growth suppressor activity in HCC PMID: 14981537
  7. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation PMID: 16996032
  8. The enhancement of FGL1 levels in vitro by IL-6 and its induction after turpentine oil injection suggest that it is an acute phase reactant. PMID: 18039467
  9. HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666

Show More

Hide All

Subcellular Location
Tissue Specificity
Under normal conditions, liver-specific.
Database Links

HGNC: 3695

OMIM: 605776

KEGG: hsa:2267

STRING: 9606.ENSP00000221204

UniGene: Hs.491143

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Leave a message

check code
© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1