Human Fibrinogen-like protein 1(FGL1) ELISA kit

Instructions
Code CSB-EL008653HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name fibrinogen-like 1
Alternative Names FGL 1 ELISA Kit; Fgl1 ELISA Kit; FGL1_HUMAN ELISA Kit; Fibrinogen like 1 ELISA Kit; Fibrinogen like protein 1 ELISA Kit; Fibrinogen related protein 1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Hepassocin ELISA Kit; Hepatocellular carcinoma related sequence ELISA Kit; Hepatocyte derived fibrinogen related protein 1 ELISA Kit; Hepatocyte-derived fibrinogen-related protein 1 ELISA Kit; HFREP 1 ELISA Kit; HFREP-1 ELISA Kit; HFREP1 ELISA Kit; HP 041 ELISA Kit; HP-041 ELISA Kit; HP041 ELISA Kit; LFIRE 1 ELISA Kit; LFIRE-1 ELISA Kit; LFIRE1 ELISA Kit; Liver fibrinogen related protein 1 ELISA Kit; Liver fibrinogen-related protein 1 ELISA Kit; MFIRE 1 ELISA Kit; MGC108569 ELISA Kit; MGC12455 ELISA Kit; MGC37822 ELISA Kit; OTTHUMP00000122468 ELISA Kit
Abbreviation FGL1
Uniprot No. Q08830
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 89  
Range % 84-94  
1:40 Average % 87  
Range % 82-93  
1:80 Average % 95  
Range % 89-99  
1:160 Average % 98  
Range % 92-104  
Recovery
The recovery of Human FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-97  
EDTA plasma (n=4) 87 82-92  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.758 2.645 2.702 2.607  
750 2.107 2.296 2.202 2.107  
375 1.601 1.524 1.563 1.468  
187.5 0.867 0.845 0.856 0.761  
94 0.475 0.483 0.479 0.384  
47 0.317 0.304 0.311 0.216  
23.5 0.181 0.176 0.179 0.084  
0 0.095 0.094 0.095    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Has hepatocyte mitogenic activity.
Gene References into Functions
  1. FGL1 promotes invasion and metastasis of gastric cancer and is associated with poor prognosis. PMID: 29845203
  2. findings highlight the crucial role of HFREP1 in insulin resistance and diabetes, and provide a potential strategy and biomarker for developing therapeutic approaches to combat these diseases. PMID: 27221093
  3. Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
  4. Here, the application of small ubiquitin-related modifier (SUMO) fusion technology in combination with four different chaperone teams on the soluble expression of recombinant HPS protein was explored and analyzed. PMID: 24084006
  5. Findings suggest that hepassocin promotes hepatic cell line L02 cells proliferation via an autocrine mechanism and inhibits HCC cells proliferation by an intracrine pathway. PMID: 21618590
  6. data demonstrated that liver-specific gene LFIRE-1/HFREP-1 was frequently downregulated and might possess growth suppressor activity in HCC PMID: 14981537
  7. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation PMID: 16996032
  8. The enhancement of FGL1 levels in vitro by IL-6 and its induction after turpentine oil injection suggest that it is an acute phase reactant. PMID: 18039467
  9. HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666

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Subcellular Location Secreted
Tissue Specificity Liver specific.
Database Links

HGNC: 3695

OMIM: 605776

KEGG: hsa:2267

STRING: 9606.ENSP00000221204

UniGene: Hs.491143

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