Product Details

Target Name

chemokine (C-C motif) ligand 23

Alternative Names

C C motif chemokine ligand 23 ELISA Kit; C-C motif chemokine 23 ELISA Kit; C6 beta chemokine ELISA Kit; CCL 23 ELISA Kit; CCL23 ELISA Kit; CCL23(30-99) ELISA Kit; CCL23_HUMAN ELISA Kit; Chemokine (C C motif) ligand 23 ELISA Kit; Chemokine CC motif ligand 23 ELISA Kit; ck beta 8 ELISA Kit; CK-beta-8 ELISA Kit; CKB 8 ELISA Kit; CKB-8 ELISA Kit; Ckb-8-1 ELISA Kit; CKB8 ELISA Kit; Hmrp-2a ELISA Kit; Macrophage inflammatory protein 3 ELISA Kit; MIP 3 ELISA Kit; MIP-3 ELISA Kit; MIP3 ELISA Kit; MPIF 1 ELISA Kit; MPIF-1 ELISA Kit; MPIF1 ELISA Kit; Myeloid progenitor inhibitory factor 1 ELISA Kit; OTTHUMP00000163948 ELISA Kit; SCYA 23 ELISA Kit; SCYA23 ELISA Kit; Small inducible cytokine A 23 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys); member 23 ELISA Kit; Small inducible cytokine subfamily A; member 23 ELISA Kit; Small-inducible cytokine A23 ELISA Kit

Abbreviation

CCL23

Uniprot No.

P55773

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39 pg/mL-2500 pg/mL

Sensitivity

9.7 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	309.863	319.761	329.703	315.796	325.721	335.691
SD	0.035	0.043	0.048	0.038	0.045	0.064
CV(%)	4.353	5.218	5.687	4.657	5.383	7.477

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MPIF-1/CCL23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-96

1:2

Average %

Range %

90-97

1:4

Average %

100

Range %

93-109

1:8

Average %

Range %

85-96

Recovery

The recovery of human MPIF-1/CCL23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-94
EDTA plasma (n=4)	95	92-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.820	2.732	2.776	2.672
1250	2.057	2.036	2.047	1.943
625	1.306	1.291	1.299	1.195
312.5	0.851	0.796	0.824	0.720
156	0.551	0.531	0.541	0.437
78	0.372	0.358	0.365	0.261
39	0.258	0.249	0.254	0.150
0	0.108	0.099	0.104
LOD 9.7 pg/ml

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This human MPIF-1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human MPIF-1 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the MPIF-1 levels in the sample and is measured at 450 nm through a microplate reader.

MPIF-1, also CCL23, MIP-3, or CKβ8, is produced by eosinophils, monocyte-derived dendritic cells, and IL-1β-activated monocytes. It serves as a chemoattractant for dendritic cells, resting T lymphocytes, and monocytes. CCL23 is associated with the pathophysiology of various inflammatory conditions such as rheumatoid arthritis, chronic rhinosinusitis, chronic kidney dysfunction, and systemic sclerosis. Circulating CCL23 levels have also been related to markers of atheroprogression, such as aortic wall thickness and plaque burden. In terms of cancer, CCL23 appears to have pro- and anti-cancer properties. A study showed that CCL23 was necessary and sufficient to promote the migration of ovarian cancer. Another study demonstrated that CCL23 is downregulated in hepatocellular carcinoma (HCC) tissues and cell lines.

Target Background

Function
(From Uniprot)

Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.

Gene References into Functions

CCL23 is posttranslationally modified by trypsin-like serine proteases and CCL23(47-117) might be a major active form of CCL23 in nasal polyps with chronic rhinosinusitis. PMID: 26560043
The coexistence of diabetes and oxidative stress independently affected CCL23 levels, while the presence of Cardiovascular disease and inflammation had no impact on its concentrations. PMID: 24995525
ADAMTS 8, CCL23, and TNFSF15 are implicated in anti-angiogenic activities PMID: 24384427
Data show CCL23 stimulates chemotaxis of human THP-1 monocytes and enhances release of MMP-2, and suggest it plays a role in atherogenesis. PMID: 21656154
Overproduction of CCL23 in nasal polyps might contribute to the pathogenesis of eosinophilic chronic rhinosinusitis with nasal polyps PMID: 21497884
Patients with systemic sclerosis who had elevated CCL23 levels had shorter disease duration, and a higher frequency of pulmonary arterial hypertension. Serum CCL23 level was increased in early phase of disease and could be a marker for disease activity. PMID: 20824279
Fresh eosinophils contained trace amounts of CCL23 protein. CCL23 was significantly released into the supernatant when the eosinophils were stimulated with GM-CSF or IL-5 but not with IFN-gamma or immobilized sIgA. PMID: 21646793
independently associated with coronary atherosclerosis PMID: 20187767
CKbeta8 transduces the chemotaxis signal through the G(i)/G(o) protein, phospholipase C, protein kinase C delta and NF-kappaB. PMID: 19951712
CCL23 promoted chemotactic migration and differentiation of endothelial cells, and neovascularization in the chick chorioallantoic membrane PMID: 15927850
May play a direct role in angiogenesis via upregulation of matrix metalloproteinase (MMP)-2 expression. PMID: 16378600
Collectively, these data suggest a link between the inducible phenotype of CCL23 expression in monocytes by the prototype Th2 molecule pair IL-4/STAT6 and the increased number of CCL23-expressing cells in skin of atopic dermatitis patients. PMID: 17371990
A peptide ligand termed SHAAGtide is cleaved from CCL23; is itself an attractant of monocytes and neutrophils in vitro; recruits leukocytes in vivo; is more potent than other natural agents with activity for FPRL1; receives authors' designation "CCR12." PMID: 17513790
This protein may participate in the malignant progression of certain human cancer cells that overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. PMID: 18258606
CCL23, M-CSF, TNFRSF9, TNF-alpha, and CXCL13 are predictive of rheumatoid arthritis disease activity and may be useful in the definition of disease subphenotypes and in the measurement of response to therapy in clinical studies. PMID: 18668547
these findings suggest that CCL23 results in up-regulation of KDR/flk-1 receptor gene transcription and protein expression and that KDR/Flk-1 up-regulation induced by CCL23 may contribute to potentiation of VEGF action in angiogenesis. PMID: 19265684

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CC

Database Links

HGNC: 10622

OMIM: 602494

KEGG: hsa:6368

UniGene: Hs.169191

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Code	CSB-E07309h
Size	96T,5×96T,10×96T
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