Human Placenta-specific protein 1(PLAC1) ELISA kit

Instructions
Code CSB-EL018108HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name placenta-specific 1
Alternative Names Cancer/testis antigen 92 ELISA Kit; CT92 ELISA Kit; Plac1 ELISA Kit; PLAC1_HUMAN ELISA Kit; Placenta specific 1 ELISA Kit; Placenta-specific protein 1 ELISA Kit
Abbreviation PLAC1
Uniprot No. Q9HBJ0
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PLAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-89
1:2Average %90
Range %86-94
1:4Average %84
Range %80-88
1:8Average %97
Range %92-101
Recovery
The recovery of human PLAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-99
EDTA plasma (n=4)8983-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.612 2.651 2.632 2.420
501.907 2.013 1.960 1.748
251.195 1.236 1.216 1.004
12.50.766 0.782 0.774 0.562
6.250.505 0.516 0.511 0.299
3.130.373 0.384 0.379 0.167
1.560.310 0.316 0.313 0.101
00.209 0.214 0.212  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Q&A and Customer Reviews

 Q&A
Q:

Regarding your ELISA kit CSB-EL018108HU, could you please advise on the following:
1. Capture Antibody: host animal species, clonality
a. Immunogen:
i. animal species immunogen sequence comes from? (Uniprot ID, if available)
ii. Expression host of immunogen
iii. native or recombinant?
iv. full-length vs. partial?
2. Detection Antibody: host animal species, clonality
a. Immunogen:
i. animal species immunogen sequence comes from? (Uniprot ID, if available)
ii. Expression host of immunogen?
iii. native or recombinant?
iv. full-length vs. partial?
3. Standard:
i. animal species the sequence comes from? (Uniprot ID, if available)
ii. Expression host?
iii. native or recombinant?
iv. full-length vs. partial?
Also, what sample types have been tested with this kit, and what were the reference values?

A:
Thanks for your inquiry!
CSB-EL018108HU
This kit is designed against full length. Pls refer to: https://www.uniprot.org/uniprot/Q9HBJ0.
Capture antibody is mouse monoclonal antibody and immunogen is NS0-derived recombinant protein,full length. It is Protein A/G purified;
Detection antibody is goat polyclonal antibody and immunogen is NS0-derived recombinant protein,partial. It is affinity purified.
Standard is NS0-derived recombinant protein.
We tested serum and plasma before and the test value is about 25ng/ml.
Pls let me know if you have any further questions. Thank you.

Target Data

Function May play a role in placental development.
Gene References into Functions
  1. The findings suggest a possible pathophysiological link between the development of Fetal growth restriction and the expression of PAPPA, PAPPA2 and PLAC-1. PMID: 29532882
  2. Screening for these five CTAs and PLAC1 by RTqPCR may offer a potentially valuable prognostic tool with good sensitivity and specificity in patients with Hepatocellular carcinoma (HCC) that may be enhanced by magneticactivated cell sorting . PMID: 28849093
  3. Study revealed that PLAC1 expression was highly expressed in non-small cell lung cancer (NSCLC) tissues, suggesting that PLAC1 may be a prognostic factor and higher risk for NSCLC patients. PLAC1 was involved in regulation of the proliferation, migration, and invasion abilities of NSCLC cells partly through regulation of epithelial-mesenchymal transition and the AKT pathway. PMID: 29138842
  4. There was no significant correlation of serum PLAC1 levels with race, age at diagnosis, body mass index (BMI) or the presence of metastatic disease. It remains to be determined whether PLAC1 serum levels can serve as a diagnostic biomarker for the presence or recurrence of disease post-surgery and/or therapy PMID: 29432428
  5. this paper shows that PLAC1 immunization does not induce infertility in mice PMID: 28351180
  6. Optimized protocol for soluble prokaryotic expression, purification and structural analysis of human placenta specific-1(PLAC1). PMID: 28315746
  7. we show that PLAC1 transcript number is significantly negatively correlated with patient survival in our samples. Thus, we suggest that characterizing tumors for TP53 mutation status, p53 protein status and PLAC1 transcription could be used to predict likely prognosis and inform treatment options in patients diagnosed with serous ovarian cancer. PMID: 28339050
  8. This study demonstrated that the protein expression of PLAC1 was significantly associated with decreased overall survival in patients with pancreatic ductal adenocarcinoma, indicating that it was a valuable prognostic marker for pancreatic ductal adenocarcinoma and might be a potential target for immunotherapy. PMID: 28618924
  9. Plac1 plays a pivotal role in the progression of HCC, and may serve as a novel therapeutic target for Hepatocellular carcinoma. PMID: 27878289
  10. PLAC1 was mainly expressed in the human villous syncytiotrophoblast (STB) layer throughout gestation, and the expression level of PLAC1 was significantly elevated during human trophoblast syncytialization. PMID: 27692364
  11. PLAC1/CP1 provides a marker for identifying gastric cancers with poor prognosis, and suggest that PLAC1/CP1 may provide a useful target for immunotherapy. PMID: 26157147
  12. circulating mRNA improved detection rate of pre-eclampsia PMID: 25138310
  13. Data suggests that PLAC1 plays an important role in human placental trophoblast invasion and migration. PMID: 24989904
  14. This data suggests that the Epstein-Barr virus-induced PLAC1 is a member of the cancer/testis group of tumor antigens. PMID: 24912876
  15. PLAC1/CP1 antigen is a possible prognostic marker of colorectal carcinoma, and PLAC1/CP1 p41-50 and PLAC1/CP1 p69-77 are novel HLA-A*0201-restricted CD8+ T cell epitopes PMID: 23604623
  16. NCOA3 is a selective co-activator of estrogen receptor alpha-mediated transactivation of PLAC1. PMID: 24304549
  17. we identified a novel specific antitrophoblast antibody, anti-PLAC1, in infertile women with repeated unexplained implantation failure. PLAC1 shows placenta-specific expression and is localized primarily in the syncytiotrophoblast. PMID: 23434395
  18. A novel HLA-A2-restricted cytotoxic T lymphocyte epitope from cancer-testis antigen PLAC1 has been identified in breast cancer. PMID: 21710262
  19. The expression of PLAC1/CP1 genes correlates with various clinical and pathologic parameters in primary colorectal carcinoma. PMID: 21215095
  20. A potential role for PLAC1 as a biomarker predictive of specific pregnancy complications, such as preeclampsia. PMID: 20509147
  21. PLAC1, a trophoblast-specific gene, is Trophoblast-specific expression throughout gestation and responsiveness to KGF are consistent with a fundamental role for PLAC1 at the maternal-fetal interface. PMID: 12412044
  22. mRNA transcripts from placenta-expressed specific gene are detectable in maternal blood and rapidly disappear after delivery PMID: 15608456
  23. PLAC1 expression is upregulated during trophoblast differentiation, localizing primarily to the differentiated syncytiotrophoblast. PMID: 15803460
  24. Plasma PLAC1 and glial cells-missing 1 mRNAs appear promising as noninvasively measurable molecular markers for pre-eclampsia PMID: 16594548
  25. In induced term pregnancies, PLAC1 mRNA in maternal blood at the beginning of the treatment correlates with time elapsed before delivery; this demonstrates that fetomaternal trafficking of nucleic acids is more consistent when labor is about to begin. PMID: 16860456
  26. Data show that PLAC1 is restricted primarily to the differentiated trophoblast, localizing to intracellular membranous compartment(s) in the apical region of the syncytiotrophoblast and associated with its apical, microvillous membrane surface. PMID: 17186554
  27. CRH, PLAC1, and selectin-P are distributed differently in preeclampsia cases compared to controls and correlate with signs of preeclampsia. PMID: 17554801
  28. RNAi-mediated silencing of PLAC1 in MCF-7 and BT-549 breast cancer cells profoundly impairs motility, migration, and invasion and induces a G1-S cell cycle block with nearly complete abrogation of proliferation. PMID: 17909063
  29. study demonstrated that PLAC1 is a novel tumor antigen with very restricted normal tissue expression; also evidence provided that PLAC1 is expressed in the tumor cells in a subset of lung cancer patients PMID: 17983203
  30. 3.8% (4/101) of HCC patients had anti-PLAC1 antibody response, suggesting the immunogenicity of PLAC1 in HCC patients. PLAC1 represents a new class of tumor associated antigen with restricted expression in placenta and cancer tissues. PMID: 18183594
  31. analysis of activation of trophoblast-specific PLAC1 in breast cancer by CCAAT/enhancer-binding protein beta (C/EBPbeta) isoform 2 PMID: 19652226
  32. In normal tissues, PLAC1 expression was restricted to the placenta while developmental pluripotency associated-2 expression was restricted to the placenta and testis. PMID: 19705800

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Subcellular Location Secreted
Protein Families PLAC1 family
Tissue Specificity Expressed in placenta. Localizes primarily to differentiated syncytiotrophoblast throughout gestation as well as to a small population of villous cytotrophoblasts. Also detected in maternal blood and rapidly disappears following delivery, but is not detec
Database Links

HGNC: 9044

OMIM: 300296

KEGG: hsa:10761

STRING: 9606.ENSP00000352173

UniGene: Hs.496811

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