Human Placenta-specific protein 1(PLAC1) ELISA kit

Instructions
Code CSB-EL018108HU
Size 96T,5×96T,10×96T

Product Details

Target Name placenta-specific 1
Alternative Names CT92, cancer/testis antigen 92
Abbreviation PLAC1
Uniprot No. Q9HBJ0
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PLAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-89
1:2Average %90
Range %86-94
1:4Average %84
Range %80-88
1:8Average %97
Range %92-101
Recovery
The recovery of human PLAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-99
EDTA plasma (n=4)8983-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.612 2.651 2.632 2.420
501.907 2.013 1.960 1.748
251.195 1.236 1.216 1.004
12.50.766 0.782 0.774 0.562
6.250.505 0.516 0.511 0.299
3.130.373 0.384 0.379 0.167
1.560.310 0.316 0.313 0.101
00.209 0.214 0.212  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

Regarding your ELISA kit CSB-EL018108HU, could you please advise on the following:
1. Capture Antibody: host animal species, clonality
a. Immunogen:
i. animal species immunogen sequence comes from? (Uniprot ID, if available)
ii. Expression host of immunogen
iii. native or recombinant?
iv. full-length vs. partial?
2. Detection Antibody: host animal species, clonality
a. Immunogen:
i. animal species immunogen sequence comes from? (Uniprot ID, if available)
ii. Expression host of immunogen?
iii. native or recombinant?
iv. full-length vs. partial?
3. Standard:
i. animal species the sequence comes from? (Uniprot ID, if available)
ii. Expression host?
iii. native or recombinant?
iv. full-length vs. partial?
Also, what sample types have been tested with this kit, and what were the reference values?

A:
Thanks for your inquiry!
CSB-EL018108HU
This kit is designed against full length. Pls refer to: https://www.uniprot.org/uniprot/Q9HBJ0.
Capture antibody is mouse monoclonal antibody and immunogen is NS0-derived recombinant protein,full length. It is Protein A/G purified;
Detection antibody is goat polyclonal antibody and immunogen is NS0-derived recombinant protein,partial. It is affinity purified.
Standard is NS0-derived recombinant protein.
We tested serum and plasma before and the test value is about 25ng/ml.
Pls let me know if you have any further questions. Thank you.
 Customer Reviews

Target Data

Function May play a role in placental development.
Subcellular Location Secreted
Protein Families PLAC1 family
Tissue Specificity Expressed in placenta. Localizes primarily to differentiated syncytiotrophoblast throughout gestation as well as to a small population of villous cytotrophoblasts. Also detected in maternal blood and rapidly disappears following delivery, but is not detec
Database Links

HGNC: 9044

OMIM: 300296

KEGG: hsa:10761

STRING: 9606.ENSP00000352173

UniGene: Hs.496811

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