Human Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

Code CSB-EL018969HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human PTGDS ELISA Kit was designed for the quantitative measurement of Human PTGDS protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.2 ng/mL .

Target Name prostaglandin D2 synthase 21kDa (brain)
Alternative Names Beta trace protein ELISA Kit; Beta-trace protein ELISA Kit; Cerebrin 28 ELISA Kit; Cerebrin-28 ELISA Kit; glutathione independent PGD synthase ELISA Kit; Glutathione independent PGD synthetase ELISA Kit; Glutathione-independent PGD synthase ELISA Kit; L PGDS ELISA Kit; Lipocalin type prostaglandin D synthase ELISA Kit; Lipocalin-type prostaglandin-D synthase ELISA Kit; LPGDS ELISA Kit; PDS ELISA Kit; PGD2 ELISA Kit; PGD2 synthase ELISA Kit; PGDS ELISA Kit; PGDS2 ELISA Kit; prostaglandin D synthase ELISA Kit; prostaglandin D2 synthase (21 kDa; brain) ELISA Kit; prostaglandin D2 synthase (brain) ELISA Kit; prostaglandin D2 synthase 21kDa (brain) ELISA Kit; Prostaglandin D2 synthase ELISA Kit; prostaglandin H2 D isomerase ELISA Kit; Prostaglandin-D2 synthase ELISA Kit; Prostaglandin-H2 D-isomerase ELISA Kit; PTGDS ELISA Kit; PTGDS_HUMAN ELISA Kit; RP23-47P18.11-006 ELISA Kit
Abbreviation PTGDS
Uniprot No. P41222
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.2 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PTGDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 93  
Range % 85-98  
1:40 Average % 90  
Range % 84-95  
1:80 Average % 98  
Range % 91-106  
1:160 Average % 97  
Range % 91-105  
Recovery
The recovery of human PTGDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 90-96  
EDTA plasma (n=4) 101 95-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.294 2.285 2.290 2.058  
25 1.806 1.854 1.830 1.598  
12.5 1.312 1.389 1.351 1.119  
6.25 0.959 0.987 0.973 0.741  
3.12 0.639 0.626 0.633 0.401  
1.56 0.438 0.424 0.431 0.199  
0.78 0.369 0.360 0.365 0.133  
0 0.233 0.231 0.232    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR.
Gene References into Functions
  1. High lipocalin-type prostaglandin D synthase expression is associated with Spontaneous intracranial hypotension. PMID: 29621631
  2. Measurement of serum BTP can be a reliable tool for detecting kidney function in neonates. PMID: 29421771
  3. Thus, our results suggest decreased NK cell-mediated eosinophil regulation, possibly through an increased level of PGD2, as a previously unrecognized link between PG dysregulation and eosinophilic inflammation in CRS. PMID: 27271931
  4. These observations suggest that tumor cell-derived inflammatory cytokines increase L-PGDS expression and subsequent PGD2 production in the tumor endothelial cells (ECs). This PGD2 acts as a negative regulator of the tumorigenic changes in tumor ECs. PMID: 29124765
  5. The cut off value for betaTP in the diagnosis and follow-up of cerebrospinal fluid leaks should be modified depending on the type of secretion (sample type), for a better diagnostic accuracy PMID: 27614217
  6. Serum levels of beta trace protein and beta 2 microglobulin can be used to predict residual kidney function in hemodialysis patients. PMID: 26924065
  7. BTP and B2M levels are less affected than serum by amputation, and should be considered for future study of GFR estimation PMID: 26800100
  8. Hematopoietic prostaglandin D synthase defines a proeosinophilic pathogenic effector human T(H)2 cell subpopulation with enhanced function PMID: 26431580
  9. levels of L-PGDS in cervicovaginal secretions of pregnant women at different stages of parturition correlate with preterm birth. PMID: 25964109
  10. Given that uPGDS levels fall after treatment of LN, uPGDS may be used to monitor the efficacy of therapy. It can also differentiate patients with active nephritis and active non renal lupus. PMID: 26211517
  11. PTGDS mRNA expression was down-regulated in rapid-cycling bipolar disorder patients in a euthymic, depressive, and manic/hypomanic state compared with healthy control subjects. PMID: 25522430
  12. Expression of MR and prostaglandin D2 synthase is strongly correlated in adipose tissues from obese patients. PMID: 25966493
  13. involved in pathogenesis of androgenetic alopecia [review] PMID: 24521203
  14. These data indicate that scalp is spatially programmed via mast cell prostaglandin D-synthase distribution in a manner reminiscent of the pattern seen in androgenetic alopecia. PMID: 24438498
  15. These results suggest that L-PGDS acted as a scavenger of biliverdin, which is a molecule not found in normal CSF. PMID: 25005874
  16. Among NSTE-ACS patients, BTP and CysC were superior to conventional renal parameters for predicting MB, and improved clinical stratification for hemorrhagic risk. PMID: 23698027
  17. Structural and dynamic insights into substrate binding and catalysis of human lipocalin prostaglandin D synthase. PMID: 23526831
  18. betaTP, beta2M, CysC, and creatinine differ in their associations with demographic and clinical factors, suggesting variation in their non-glomerular filtration rate determinants. PMID: 23335043
  19. All-cause and cardiovascular mortality are strongly associated with beta-trace protein marker levels and beta-microglobulin in a representative sample of US adults. PMID: 23518194
  20. The results we obtained from mice led to our investigation of PTGDS in 29 human cryptorchid patients but we failed to find any mutation that supported an involvement of this gene in human testicular descent. PMID: 23076868
  21. no overall evidence of an association between wireless phone use and serum concentrations of beta-trace protein PMID: 22989106
  22. Elevated plasma level of beta-trace protein associated with all cause of death in patients with acute coronary syndrome. PMID: 22818840
  23. The serum level of beta-trace protein is an independent predictor of death and cardiovascular disease mortality in incident hemodialysis patients. PMID: 22745274
  24. Low PTGDS expression is associated with testis cancer. PMID: 22960220
  25. L-PGDS expression may contribute to the restricted proliferation of epidermal melanocytes, but conversely its overexpression may reflect the dysregulated proliferation of melanoma cells. PMID: 22299829
  26. The gene coding for PTGDS was found to be more expressed in patients with attention deficit hyperactivity disorder (ADHD)relative to patients with bipolar disorder indicating a possible link with the differential etiology of ADHD. PMID: 22370065
  27. Data suggest that L-PGDS binds small lipophilic ligands with both high-affinity and low-affinity interactions; molecular models are proposed from studies that include binding of hemin, biliverdin, and bilirubin. PMID: 22677050
  28. Ascites and pleural effusion contain high concentrations of beta-TP that exceed the levels in corresponding plasma PMID: 21501068
  29. Urinary PGDS, not ZA2G, may serve as a biomarker for active LN and upon validation in larger studies, may become the non-invasive test to evaluate the disease activity in future management of LN. PMID: 22498882
  30. NM 000954 NM 000954 PMID: 22342541
  31. these results demonstrate that L-PGDS protected against neuronal cell death by scavenging reactive oxygen species without losing its ligand-binding function PMID: 22248185
  32. Beta-trace protein can be used as an alternative diagnostic tool to detect moderate or severe glomerular filtrate rate reduction in patients after liver transplantation. PMID: 21745310
  33. two genes involved in cardiovascular diseases, ADORA1 and PTGDS, were differentially up-regulated in epicardial adipose tissue compared to mediastinal and subcutaneous adipose tissue PMID: 21603615
  34. Lipocalin-type prostaglandin D synthase is associated with coronary vasospasm and vasomotor reactivity in response to acetylcholine PMID: 21325722
  35. Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma. PMID: 21271676
  36. RBP-4, lipocalin-2 and L-PGDS do not regulate insulin sensitivity in healthy men. Rather their expression seemed to reflect inflammatory activity and were inversely correlated with alcohol intake and serum HDL levels. PMID: 21104585
  37. Report the presence of L-PGDS in the COX-2-expressing cells in the mucosa of active ulcerative colitis patients in parallel with disease activity. PMID: 21163901
  38. It may be feasible to test for perilymphatic fluid fistula using PTGDS in samples from the tympanic cavity. PMID: 21192373
  39. Met64 seems to function as a kinetic clamp, pushing the thiol group of Cys65 close to the site of nucleophilic attack during catalysis. PMID: 20667974
  40. Using RT-PCR we demonstrated that L-PGDS gene expression decreased proportionately with tumor progression in lung cancer. PMID: 20144489
  41. L-PGDS may fine-tune the retinoic acid signalling in melanocytes. PMID: 20403807
  42. This study included 62 persons aged 18-30 years and cell phone exposure. EMF emissions may down-regulate the synthesis of beta-trace protein. PMID: 20596612
  43. protein levels in nasal fluids can serve for diagnosis of cerebrospinal fluid leak PMID: 19958607
  44. This beta-trace protein based formula was found to estimate GFR with reasonable precision PMID: 19949816
  45. The expression of H-PGDS in human dendritic cells (DCs) and the regulatory mechanisms by which DCs produce prostaglandin D2, is demonstrated. PMID: 20008150
  46. As the CSF is in contact with axons and mitochondria of the optic nerve, we postulate that a change in the concentration of CSF protein such as L-PGDS could exercise a harmful effect on these structures. PMID: 19598000
  47. Pronounced eosinophilia and Th2 cytokine release in human lipocalin-type prostaglandin D synthase transgenic mice PMID: 11751991
  48. Study performed in patients with kidney function ranging from normal to advanced renal failure suggests that serum beta-trace protein is an indicator of impaired glomerular filtration rate. PMID: 12900000
  49. The circadian lipocalin-type PGDS pattern and its suppression by total sleep deprivation indicate an interaction of the prostaglandin D system and human sleep regulation. PMID: 15453544
  50. Shear stress induces l-PGDS expression by transcriptional activation through the AP-1 binding site. PMID: 15718494

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Subcellular Location Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm, perinuclear region. Secreted. Note=Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prosta
Database Links

HGNC: 9592

OMIM: 176803

KEGG: hsa:5730

STRING: 9606.ENSP00000360687

UniGene: Hs.446429

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