CUSABIO's human syndecan-1 (SDC1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for quantitatively measuring human SDC1 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of SDC1 in samples. The color intensity is measured at 450 nm via a microplate reader.
SDC1, alternatively referred to as CD138, is a heparan sulfate proteoglycan that is expressed predominantly on epithelia and leukocytes and is involved in cell proliferation, cell growth, metastasis, angiogenesis, cell-extracellular matrix interactions, inflammatory response, and wound healing. It can bind to integrins, growth factors, cytokines as well as chemokines. SDC1 plays a major role in the clearance of triglycerides. It is implicated in the differentiation and prognosis of various tumors. Dysregulated expression of SDC1 has been found in different cancer types and contributes to cancer progression by promoting cell proliferation, metastasis, invasion, and angiogenesis, and is associated with relapse through chemoresistance.