Human Ubiquitin-like modifier-activating enzyme ATG7(ATG7) ELISA kit

Code CSB-EL002294HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
ATG7 autophagy related 7 homolog (S. cerevisiae)
Alternative Names
1810013K23Rik ELISA Kit; Apg 7 ELISA Kit; APG7 autophagy 7 like ELISA Kit; APG7 autophagy 7-like (S. cerevisiae) ELISA Kit; APG7 like ELISA Kit; APG7; S. cerevisiae; homolog of ELISA Kit; APG7-like ELISA Kit; APG7L ELISA Kit; ATG 7 ELISA Kit; ATG12-activating enzyme E1 ATG7 ELISA Kit; ATG7 ELISA Kit; ATG7 autophagy related 7 homolog (S. cerevisiae) ELISA Kit; ATG7 autophagy related 7 homolog ELISA Kit; ATG7_HUMAN ELISA Kit; Atg7l ELISA Kit; Autophagy 7; S. cerevisiae; homolog of ELISA Kit; Autophagy related protein 7 ELISA Kit; Autophagy-related 7 (yeast) ELISA Kit; Autophagy-related protein 7 ELISA Kit; DKFZp434N0735 ELISA Kit; GSA 7 ELISA Kit; GSA7 ELISA Kit; hAGP7 ELISA Kit; Ubiquitin activating enzyme E1 like protein ELISA Kit; Ubiquitin-activating enzyme E1-like protein ELISA Kit; Ubiquitin-like modifier-activating enzyme ATG7 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
28 pg/mL-1800 pg/mL
7 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human ATG7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-90
1:2 Average % 101
Range % 97-104
1:4 Average % 98
Range % 94-102
1:8 Average % 105
Range % 101-109
The recovery of human ATG7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 81-89
EDTA plasma (n=4) 101 97-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1800 2.539 2.639 2.589 2.466
900 2.058 2.004 2.031 1.908
450 1.275 1.368 1.322 1.199
225 0.701 0.724 0.713 0.590
112.5 0.401 0.394 0.398 0.275
56 0.253 0.248 0.251 0.128
28 0.190 0.187 0.189 0.066
0 0.124 0.122 0.123  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human ATG7 ELISA Kit was designed for the quantitative measurement of Human ATG7 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.

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Target Background

(From Uniprot)
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner.
Gene References into Functions
  1. Silencing of ATG7 downregulated MALAT1 and ATG7 reciprocally regulated each other by miR-200a. PMID: 29794480
  2. miR-210 inhibits autophagy via silencing of ATG7. PMID: 28667916
  3. miR-106a suppresses tumor cells death in colorectal cancer through targeting ATG7. PMID: 27981410
  4. PSMD10/gankyrin has a role in inducing autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression PMID: 25905985
  5. the ATG5-ATG7-NCOA4 autophagic pathway has a role in ferroptosis PMID: 27245739
  6. knockdown of autophagy genes ATG5 or ATG7 resulted in reduced hematopoietic stem/progenitor cell frequencies in vitro as well as in vivo. PMID: 26930546
  7. Nine of the 32 (28.1%) iCCA patients had gene mutations at chromosome 3p, totaling 11 mutations across five genes. Those included five (15.6%) BAP1 mutations, two each (6.3%) of CACNA2D3 and RASSF1 mutations, and one each (3.1%) of ATG7 and PLCD1 mutations. Six (18.8%) cases had concurrent loss of chromosome 3p and gene mutations. PMID: 29122566
  8. knockdown of ATG7 results in decreased glycolysis and increased flux of labeled carbons through the mitochondrial tricarboxylic acid cycle. PMID: 27168493
  9. SNP rs11706903 in ATG7 was not associated with systemic lupus erythematosus I Chinese Han population. PMID: 26420661
  10. The G allele of rs8154 was correlated with breast cancer patients poorer survival. PMID: 28669927
  11. low serum ATG7 is associated with ulcerative colitis PMID: 27881025
  12. The U2AF35(S34F) mutation alters interaction with CFIm59, leading to increased use of a distal cleavage and polyadenylation site in the ATG7 pre-mRNA, decreasing levels of ATG7 protein and defective autophagy, ultimately leading to transformation. PMID: 27184077
  13. the inhibition of Atg7 appears to be a valid strategy to enhance chemosensitivity, and it could indeed improve outcomes in acute myeloid leukemia therapy. PMID: 27268264
  14. Our data demonstrate that HOTAIR promotes the activation of autophagy in HCC cells by upregulating the expression of the autophagy-related genes ATG3 and ATG7. PMID: 27301338
  15. Autophagy-related gene ATG7 was validated as a target of miR-17 seed family. PMID: 27501757
  16. the upregulation of autophagy-related gene (Atg) and wingless/int1 (Wnt) signaling during BMP-2-mediated human osteoblastic differentiation, was examined. PMID: 27397580
  17. Aldo was revealed to induce autophagy, as indicated by the increased conversion from microtubuleassociated protein 1A/1Blight chain 3 (LC3)I to LC3II, the increased expression levels of autophagyrelated gene 7 (Atg7) and the increased degradation of p62, which was accompanied by MC proliferation PMID: 27748808
  18. PVT1 overexpression increased the expression of Atg7 and Beclin1 by targeting miR-186, which induced protective autophagy, thus promoting glioma vascular endothelial cell proliferation, migration, and angiogenesis. Therefore, PVT1 and miR-186 can provide new therapeutic targets for future anti-angiogenic treatment of glioma PMID: 28351322
  19. Genetic polymorphisms of ATG5 and ATG7 could contribute to neutrophilic airway inflammation in the pathogenesis of adult asthma. PMID: 26701229
  20. We have identified ATG7 as a potential tissue biomarker distinguishing active EoE from normal, EoE remission, and GERD. PMID: 26785669
  21. These observations indicate that Atg7-mediated autophagy is dispensable for retinoid recycling and A2E deposition; however, autophagy plays a role in coping with stress caused by A2E accumulation. PMID: 26468292
  22. Inhibition of Atg7-mediated autophagy within the epithelium may prevent the development and progression of colorectal cancer in genetically predisposed patients. PMID: 26214133
  23. The region of human ATG3 that interacts with ATG7 is precisely identified using nuclear magnetic resonance. PMID: 26043688
  24. the Atg7.caspase-9 complex performs a dual function of linking caspase-9 to the autophagic process while keeping in check its apoptotic activity. PMID: 24362031
  25. a protective mechanism for Atg4B, Atg3 and LC3-Atg3 conjugate from being inappropriately sequestered into p62 aggregates PMID: 24023838
  26. the polymorphism is associated with a 6-years earlier disease onset PMID: 23894380
  27. 2-methoxyestradiol-dependent autophagosome formation in osteosarcoma cells requires ATG7 expression. PMID: 23527187
  28. Data indicate mitogen lacritin stimulates FOXO3-ATG101 and FOXO1-ATG7 autophagic coupling and restores metabolic homeostasis. PMID: 23640897
  29. the sequence variants within the ATG7 gene promoter identified in PD patients may change ATG7 protein levels, which in turn would influence autophagic activity, contributing to Parkinson disease onset as a risk factor. PMID: 23295909
  30. Heat shock factor 1 (HSF1) controls chemoresistance and autophagy through transcriptional regulation of autophagy-related protein 7 (ATG7) PMID: 23386620
  31. tetrandrine transcriptionally regulated the expression of autophagy related gene 7 (ATG7), which promoted tetrandrine-induced autophagy. PMID: 22927446
  32. when nutrients are limited, Atg7 regulates p53-dependent cell cycle and cell death pathways PMID: 22499945
  33. The FAP motif of ATG7 is indispensable for formation of the ATG3-LC3 E2-substrate intermediate through the interaction of ATG7 with ATG3. PMID: 22170151
  34. Data show that both EGFR-TKIs increased ATG5 and ATG7 at the mRNA or protein levels (Figure 3), confirming the induction of autophagy by EGFR-TKIs. PMID: 21655094
  35. Knockdown of autophagy-related protein beclin 1 (BCN1) or autophagy-related protein 7 (ATG7) in immortalized human hepatocytes (IHHs) inhibits hepatitis C virus growth. PMID: 21274862
  36. Data show that polymorphism in the Atg7 gene that substitutes alanine for valine (V471A) was associated with an earlier disease onset of 4 years. PMID: 20697744
  37. In response to stress, FoxO1 was acetylated by dissociation from sirtuin-2 (SIRT2), a NAD(+)-dependent histone deacetylase, and the acetylated FoxO1 bound to Atg7. PMID: 20543840
  38. Data suggest that apoptosis-deficient cells rely on autophagy for cell death after Pc 4-PDT and that the strong activation of LC3 maturation in response to PDT could occur even in cells with limited or no Atg7 expression. PMID: 20083906
  39. Data show that proteasome inhibition promoted autophagosome formation, stimulated autophagic flux, and upregulated expression of the autophagy-specific genes ATG5 and ATG7. PMID: 19881538
  40. Murine Atg8L/Apg8L modification is mediated by human Atg7. PMID: 16704426
  41. The suppression of unfolded protein response or the suppression of expression of LC3 or Atg7, a protein that mediates LC3 lipidation, suppressed HCV replication. PMID: 18688877
  42. Knockdown of p300 reduces acetylation of Atg5, Atg7, Atg8, and Atg12, although overexpressed p300 increases the acetylation of these same proteins. PMID: 19124466
  43. The UBQLN protective effect requires the autophagy-related genes ATG5 and ATG7, two essential components of autophagy. PMID: 19148225

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Subcellular Location
Cytoplasm. Preautophagosomal structure.
Protein Families
ATG7 family
Tissue Specificity
Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Database Links

HGNC: 16935

OMIM: 608760

KEGG: hsa:10533

STRING: 9606.ENSP00000346437

UniGene: Hs.38032

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