Human Ubiquitin-like modifier-activating enzyme ATG7(ATG7) ELISA kit

Code CSB-EL002294HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O95352
Lead Time 5-7 working days
Abbreviation ATG7
Protein Biological Process 1 Apoptosis/Autophagy
Target Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Alias APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like|ubiquitin activating enzyme E1-like protein
Species Homo sapiens (Human)
Protein Biological Process 3 Autophagy
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
HGNC 16935
RGD 1304817
MGI 1921494
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ATG7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-90
1:2 Average % 101
Range % 97-104
1:4 Average % 98
Range % 94-102
1:8 Average % 105
Range % 101-109
Recovery
The recovery of human ATG7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 81-89
EDTA plasma (n=4) 101 97-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1800 2.539 2.639 2.589 2.466
900 2.058 2.004 2.031 1.908
450 1.275 1.368 1.322 1.199
225 0.701 0.724 0.713 0.590
112.5 0.401 0.394 0.398 0.275
56 0.253 0.248 0.251 0.128
28 0.190 0.187 0.189 0.066
0 0.124 0.122 0.123  
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Function E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Subcellular Location Cytoplasm, Preautophagosomal structure
Protein Families ATG7 family
Tissue Specificity Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Database Links

HGNC: 16935

OMIM: 608760

KEGG: hsa:10533

STRING: 9606.ENSP00000346437

UniGene: Hs.38032

Pathway Autophagy

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