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| Code | CSB-E09745h |
| Size | 96T,5×96T,10×96T |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human omentin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:800 | Average % | 102 | ||||
| Range % | 93-110 | |||||
| 1:1600 | Average % | 95 | ||||
| Range % | 82-99 | |||||
| The recovery of human omentin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 80-100 | ||||
| EDTA plasma (n=4) | 96 | 90-105 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human ITLN1 ELISA Kit allows for the in vitro quantitative determination of ITLN1 concentrations in serum, plasma, cell culture supernates, or tissue homogenates. This kit exclusively recognizes human ITLN1. Omentin, also called intelectin-1 (ITLN1), is an adipokine with an anti-inflammatory function and is generated by the stromal-vascular fraction of visceral adipose tissue. ITLN1 has recently been identified as a secretory and galactofuranose-binding lectin and is primarily produced in the gastrointestinal goblet cells and omentum. Evidence has shown that ITLN1 is involved in the gut immune defense against microorganisms, insulin-stimulated glucose uptake, chronic obstructive pulmonary disease, and asthma. ITLN1 also participates in neoplastic diseases such as pleural mesothelioma and gastric and prostate cancer.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. ITLN1 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled ITLN1 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with ITLN1 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.
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