Mouse Amylin ELISA Kit

Instructions
Code CSB-E08538m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name islet amyloid polypeptide
Alternative Names IappIslet amyloid polypeptide ELISA Kit; Amylin ELISA Kit; Diabetes-associated peptide ELISA Kit; DAP ELISA Kit
Abbreviation IAPP
Uniprot No. P12968
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse amylin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 103-110
1:2 Average % 89
Range % 85-93
1:4 Average % 102
Range % 98-106
1:8 Average % 85
Range % 81-89
Recovery
The recovery of mouse amylin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 95-103
EDTA plasma (n=4) 86 83-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 1.949 2.012 1.981 1.868
800 1.523 1.502 1.513 1.400
400 1.021 0.998 1.010 0.897
200 0.653 0.672 0.663 0.550
100 0.465 0.438 0.452 0.339
50 0.297 0.301 0.299 0.186
25 0.183 0.180 0.182 0.069
0 0.112 0.114 0.113  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
Gene References into Functions
  1. These data provide for the first time evidences for the toxic nature of oligomeric assemblies of murine amylin and its existence in wild-type, non-transgenic mice. PMID: 29372531
  2. Circulating aggregated amylin accumulates preferentially in male vs. female hearts and its effects on myocyte Ca(2+) cycling do not require diabetic remodeling of the myocardium. PMID: 29066284
  3. Results suggest that amylin directly acts, through a p-ERK-mediated process, on POMC neurons to enhance arcuate nucleus-paraventricular nucleus alphaMSH pathway development. PMID: 29467172
  4. These data suggest participation by both soluble and fibrillar aggregates in IAPP-induced islet inflammation. IAPP-induced activation of TLR2 and secretion of IL-1 may be important therapeutic targets to prevent amyloid-associated beta cell dysfunction. PMID: 26786104
  5. Hypothalamic amylin is transcriptionally regulated by leptin, that it can act directly on ObRb neurons in concert with leptin, and that it regulates feeding. PMID: 26655697
  6. Matrix Metalloproteinase-9 Protects Islets from Amyloid-induced Toxicity. PMID: 26483547
  7. Study the physiologic actions of IAPP on pancreatic beta cells, which secrete this peptide together with insulin upon glucose stimulation. Explore the signaling pathways and mitogenic actions of IAPP on beta cells. PMID: 25808537
  8. deletion of the DeltaN isoforms of p63 or p73 leads to metabolic reprogramming and regression of p53-deficient tumours through upregulation of IAPP, the gene that encodes amylin, a 37-amino-acid peptide co-secreted with insulin by the beta cells of the pancreas PMID: 25409149
  9. The stability, conformational dynamics and association force of different single-layer models of the full-length wild-type and glycine mutants of amylin, were investigated. PMID: 24028418
  10. MMP-9 constitutes an endogenous islet protease that limits islet amyloid deposition and its toxic effects via degradation of hIAPP. PMID: 23229548
  11. Data suggest that amylin and leptin play additive roles in regulating energy homeostasis via activation of overlapping signalling pathways; mechanisms may be different in hypothalamus, muscle, and liver and in cases of endoplasmic reticulum stress. PMID: 21997794
  12. These findings point to a potential for Drosophila melanogaster to serve as a model system for studies of hproIAPP and hIAPP expression with subsequent aggregation and developed pathology. PMID: 21695120
  13. This study demonstrated that amylin inactivation leads to a low bone mass due to an increase in bone resorption whereas bone formation is unaffected. PMID: 14970190
  14. Fatty acids differently regulate amylin and insulin expression and induce both amylin and insulin release PMID: 19843871
  15. Amylin/leptin's marked weight- and fat-reducing effects are due to activation of intrinsic synergistic neuronal signaling pathways. PMID: 19875640
  16. expression pattern of IAPP and prohormone convertase 1/3 reveals a distinctive set of endocrine cells in the embryonic pancreas PMID: 12049785
  17. IAPP has complex effects on islet hormone secretion serving as an inhibitor of glucagon release and having a dual action on insulin secretion. PMID: 12609749
  18. Functional role of the -132 G/A mutation reported in the promoter region of the IAPP gene. PMID: 15243700
  19. analysis of pro-islet amyloid polypeptide processing in the constitutive and regulated secretory pathways of beta cells PMID: 15802374
  20. Reduced expression in gastrin-cholecystokinin knockout mice. PMID: 16002530
  21. Non-amyloidogenic mouse IAPP has a pronounced ability to insert into phospholipid monolayers, even at lipid packing conditions that exceed the conditions that occur in biological membranes. PMID: 16403520
  22. This study demonstrates that amylin deficiency exerts effects on bone during growth that are sex-dependent and suggest a possible interaction between amylin and testosterone, growth factors, or cytokines to regulate bone cell metabolism. PMID: 16830202
  23. Findings demonstrate that inhibition of IAPP synthesis prevents amyloid formation and beta-cell death in cultured human islets. PMID: 18694973

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Subcellular Location Secreted
Protein Families Calcitonin family
Database Links

KEGG: mmu:15874

STRING: 10090.ENSMUSP00000043956

UniGene: Mm.415

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