Protein purification method: affinity chromatography


The recombinant protein research group conducted a regular meeting in October. Wang Junyu and Qiu Yu made a speech respectively.

Wang Junyu mainly talked about the applications of protein purification as well as the protein purification method, affinity chromatography. Protein purification can help study the structure and function of protein; extract active enzyme or protein; and develop drugs and food additives.

One important method of protein purification is affinity chromatography, which is based on the specificity of biological affinity between biological molecules and their ligands. In this method, the ligands are fixed on the solid phase carrier. When the liquid sample flows through, the particular biological molecules that have affinity with the ligands are absorbed by the column and separated from other impurities.

In recent decades, the affinity chromatography technology has been developing rapidly. Affinity chromatography is widely used in scientific research, especially in proteomics. In addition to proteins, it can be also used to purify other biological molecules such as enzymes, inhibitors, antibodies, antigens, hormones, hormone receptors, glycoproteins, nucleic acids and polysaccharides, etc..

Qiu Yu talked made an analysis of plasmid extraction from all aspects at the meeting. He explained the reasons for low rate and failure of plasmid extraction, and provided solutions to these problems.
Cite this article

CUSABIO team. Protein purification method: affinity chromatography. https://www.cusabio.com/c-13847.html
 
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