Mouse Carnitine O-palmitoyltransferase 1, liver isoform(CPT1A) ELISA kit

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Target Background

Product Details

Target Name

carnitine palmitoyltransferase 1A (liver)

Alternative Names

Cpt1a; Cpt-1; Cpt1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A

Abbreviation

CPT1A

Uniprot No.

P97742

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell lysates, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CPT1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-100
1:2	Average %	96
	Range %	87-102
1:4	Average %	90
	Range %	85-99
1:8	Average %	93
	Range %	87-99

Recovery

The recovery of mouse CPT1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-104
EDTA plasma (n=4)	93	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.006	2.034	2.020	1.843
200	1.372	1.385	1.379	1.202
100	0.735	0.765	0.750	0.573
50	0.431	0.442	0.437	0.260
25	0.286	0.291	0.289	0.112
12.5	0.237	0.248	0.243	0.066
6.25	0.215	0.223	0.219	0.042
0	0.174	0.179	0.177

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse CPT1A ELISA Kit was designed for the quantitative measurement of Mouse CPT1A protein in serum, plasma, cell lysates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Background

Function
(From Uniprot)

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in hepatic triglyceride metabolism.

Gene References into Functions

Alogliptin increased hepatic mRNA expression levels associated with fatty acid oxidation. In addition, the results of the present study suggested that ALO promotes CPT1a expression via Thr172 phosphorylation of AMPKalpha. PMID: 29512720
Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype. PMID: 28330968
beneficial effects of muscle CPT1mt expression suggest that a direct modulation of the malonyl-CoA/CPT1 partnership in skeletal muscle could represent a potential strategy to prevent obesity-induced insulin resistance PMID: 27507552
The data suggest that AMPK is not required for the regulation of the intermediate filament interaction with CPT-I during exercise. PMID: 27941154
These results suggest that n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. PMID: 27110821
these results demonstrated that L-carnitine ameliorated liver inflammation and serum pro-inflammatory markers in cancer cachexia through regulating CPT I-dependent PPARg signaling PMID: 26647854
Data show that peroxisome-proliferator-activated receptor beta/delta (PPARbeta/delta) activation restored the lipid-induced endothelial dysfunction by up-regulation of carnitine palmitoyltransferase)-1 (CPT-1). PMID: 26253087
Our results indicated that exercise-induced CPT1b expression was at least in part mediated by HDAC5/MEF2A interaction. PMID: 25213552
Data indicate that muscle mutated carnitine palmitoyltransferase 1 (CPT1mt) expression enhanced mitochondrial fatty acid oxidation (mFAO) capacity. PMID: 25713059
Potential epigenetic mechanisms underlying transcriptional regulation of Cpt1alpha in hepatic steatosis occur in response to binge ethanol administration. PMID: 23905631
an environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A PMID: 21990363
miR-370 acting via miR-122 may have a causative role in the accumulation of hepatic triglycerides by modulating initially the expression of SREBP-1c, DGAT2, and Cpt1alpha. PMID: 20124555
Results show that CREB and HNF4alpha bind the CPT promoter, that PGC-1 is involved in liver carnitine palmitoyltransferase I (CPT) gene activation by cylic AMP, and that PGC-1 acts to coordinate the process of metabolic adaptation in the liver. PMID: 12107181
role of thyroid hormone response unit formed between the promoter and first intron in mediating liver-specific induction by thyroid hormone PMID: 12493735
Malonyl-CoA/CPTI interaction is a component of a metabolic signaling network that controls insulin secretion. PMID: 15677504
modulation of CPT1A expression by PI3K-dependent signaling is the major mechanism by which cells suppress beta-oxidation during anabolic growth PMID: 17030509
These results suggested that astaxanthin promoted lipid metabolism rather than glucose utilization during exercise via CPT I activation, which led to improvement of endurance and efficient reduction of adipose tissue with training. PMID: 18082622

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Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein.

Protein Families

Carnitine/choline acetyltransferase family

Database Links

KEGG: mmu:12894

STRING: 10090.ENSMUSP00000025835

UniGene: Mm.18522

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Code	CSB-EL005922MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.