Mouse Carnitine O-palmitoyltransferase 1, liver isoform(CPT1A) ELISA kit

Instructions
Code CSB-EL005922MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name carnitine palmitoyltransferase 1A (liver)
Alternative Names Cpt1a ELISA kit; Cpt-1 ELISA kit; Cpt1Carnitine O-palmitoyltransferase 1 ELISA kit; liver isoform ELISA kit; CPT1-L ELISA kit; EC 2.3.1.21 ELISA kit; Carnitine O-palmitoyltransferase I ELISA kit; liver isoform ELISA kit; CPT I ELISA kit; CPTI-L ELISA kit; Carnitine palmitoyltransferase 1A ELISA kit
Abbreviation CPT1A
Uniprot No. P97742
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CPT1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %83-100
1:2Average %96
Range %87-102
1:4Average %90
Range %85-99
1:8Average %93
Range %87-99
Recovery
The recovery of mouse CPT1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9786-104
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.006 2.034 2.020 1.843
2001.372 1.385 1.379 1.202
1000.735 0.765 0.750 0.573
500.431 0.442 0.437 0.260
250.286 0.291 0.289 0.112
12.50.237 0.248 0.243 0.066
6.250.215 0.223 0.219 0.042
00.174 0.179 0.177
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism.
Gene References into Functions
  1. Alogliptin increased hepatic mRNA expression levels associated with fatty acid oxidation. In addition, the results of the present study suggested that ALO promotes CPT1a expression via Thr172 phosphorylation of AMPKalpha. PMID: 29512720
  2. Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype. PMID: 28330968
  3. beneficial effects of muscle CPT1mt expression suggest that a direct modulation of the malonyl-CoA/CPT1 partnership in skeletal muscle could represent a potential strategy to prevent obesity-induced insulin resistance PMID: 27507552
  4. The data suggest that AMPK is not required for the regulation of the intermediate filament interaction with CPT-I during exercise. PMID: 27941154
  5. These results suggest that n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. PMID: 27110821
  6. these results demonstrated that L-carnitine ameliorated liver inflammation and serum pro-inflammatory markers in cancer cachexia through regulating CPT I-dependent PPARg signaling PMID: 26647854
  7. Data show that peroxisome-proliferator-activated receptor beta/delta (PPARbeta/delta) activation restored the lipid-induced endothelial dysfunction by up-regulation of carnitine palmitoyltransferase)-1 (CPT-1). PMID: 26253087
  8. Our results indicated that exercise-induced CPT1b expression was at least in part mediated by HDAC5/MEF2A interaction. PMID: 25213552
  9. Data indicate that muscle mutated carnitine palmitoyltransferase 1 (CPT1mt) expression enhanced mitochondrial fatty acid oxidation (mFAO) capacity. PMID: 25713059
  10. Potential epigenetic mechanisms underlying transcriptional regulation of Cpt1alpha in hepatic steatosis occur in response to binge ethanol administration. PMID: 23905631
  11. an environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A PMID: 21990363
  12. miR-370 acting via miR-122 may have a causative role in the accumulation of hepatic triglycerides by modulating initially the expression of SREBP-1c, DGAT2, and Cpt1alpha. PMID: 20124555
  13. Results show that CREB and HNF4alpha bind the CPT promoter, that PGC-1 is involved in liver carnitine palmitoyltransferase I (CPT) gene activation by cylic AMP, and that PGC-1 acts to coordinate the process of metabolic adaptation in the liver. PMID: 12107181
  14. role of thyroid hormone response unit formed between the promoter and first intron in mediating liver-specific induction by thyroid hormone PMID: 12493735
  15. Malonyl-CoA/CPTI interaction is a component of a metabolic signaling network that controls insulin secretion. PMID: 15677504
  16. modulation of CPT1A expression by PI3K-dependent signaling is the major mechanism by which cells suppress beta-oxidation during anabolic growth PMID: 17030509
  17. These results suggested that astaxanthin promoted lipid metabolism rather than glucose utilization during exercise via CPT I activation, which led to improvement of endurance and efficient reduction of adipose tissue with training. PMID: 18082622

Show More

Hide All

Subcellular Location Mitochondrion outer membrane, Multi-pass membrane protein
Protein Families Carnitine/choline acetyltransferase family
Database Links

KEGG: mmu:12894

STRING: 10090.ENSMUSP00000025835

UniGene: Mm.18522

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1