Mouse Mitochondrial brown fat uncoupling protein 1(UCP1) ELISA kit

Instructions
Code CSB-EL025554MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name uncoupling protein 1 (mitochondrial, proton carrier)
Alternative Names Ucp1 ELISA Kit; Slc25a7 ELISA Kit; Ucp ELISA Kit; Mitochondrial brown fat uncoupling protein 1 ELISA Kit; UCP 1 ELISA Kit; Solute carrier family 25 member 7 ELISA Kit; Thermogenin ELISA Kit
Abbreviation UCP1
Uniprot No. P12242
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 4.68 pg/mL-300 pg/mL
Sensitivity 1.17 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %86-97
1:2Average %95
Range %91-100
1:4Average %89
Range %85-96
1:8Average %89
Range %83-94
Recovery
The recovery of mouse UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9186-96
EDTA plasma (n=4)10296-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
3002.388 2.466 2.427 2.267
1501.702 1.702 1.702 1.542
750.937 0.989 0.963 0.803
37.50.641 0.636 0.639 0.479
18.750.340 0.368 0.354 0.194
9.370.287 0.288 0.288 0.128
4.680.228 0.211 0.220 0.060
00.153 0.166 0.160
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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 Q&A
Q:

Please provide the following information regardingMouse Mitochondrial brown fat uncoupling protein 1, UCP1 ELISA Kit (CSB-EL025554MO)
2) Is the capture antibody a monoclonal or polyclonal?
3) What is the host of the capture antibody?
4) What’s the immunogen or immunogen range of the capture antibody?
5) What’s is the species of the immunogen of the capture antibody, human or something else?
6) Is the detection antibody a monoclonal or polyclonal?
7) What is the host of the detection antibody?
8) What is the immunogen or immunogen range of the detection antibody?
9) What is the species of the immunogen of the detection antibody? Human or something else?
10) What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
11) If recombinant protein, is it full length or partial?
12) What is the species of the standard– human or something else?

A:
Thanks for your inquiry!
CSB-EL025554MO
This kit is designed against extracellular domain. Pls refer to: https://www.uniprot.org/uniprot/P12242.
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody.
The antibody's immunogen is an extracellular sequence expressed in tandem (using recombinant protein expressed by 293 cells).
The standard is 293 cell-derived recombinant protein.

Target Data

Function Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance
Gene References into Functions
  1. This study highlights the novel mechanism of HDAC3-regulated Ucp1 expression during beta-adrenergic receptor stimulation. PMID: 30126161
  2. These findings suggested that HDAC6 contributes to mitochondrial thermogenesis in brown adipose tissue by increasing UCP1 expression through cAMP-PKA signaling pathway. PMID: 29890133
  3. Findings demonstrated that one bout of both uphill and downhill exercise trainings as well as 8 weeks of training could increase the expression of PGC-1alpha and FNDC5 genes in the muscle tissues and the UCP1 gene in the subcutaneous adipose tissue. PMID: 30218749
  4. To identify putatively causal regulators, we performed transcription factor binding site overrepresentation analyses in active chromatin regions and prioritized factors based on their expression correlation with the bona-fide brown adipogenic marker Ucp1 across multiple mouse and human datasets. PMID: 28181539
  5. Pyruvate induces torpor in obese mice. Pyruvate does not induce torpor in lean mice but results in the activation of brown adipose tissue with an increase in the level of uncoupling protein-1. PMID: 29311303
  6. striking reduction of mitochondrial electron transport chain components in mice genetically lacking UCP1 PMID: 28630339
  7. UCP1 cannot be fully inhibited by all adenine nucleotides tested. Phosphate is a novel inhibitor of UCP3 and UCP1. Conserved arginines in the PN-binding pocket are involved in the inhibition of UCP1 and UCP3 to different extents. Fatty acids compete with all PNs bound to UCP1. PMID: 29212043
  8. UCP1 mRNA expression is increased significantly with 10 muM of ACTH. PMID: 28375845
  9. Data suggest that triiodothyronine and high glucose signal coordinately to up-regulate ChREBP, Ucp1, Glut4, and Fasn in brown adipocytes; ChREBP plays role as a central regulator of brown adipocyte activity/energy metabolism. (ChREBP = carbohydrate-responsive element-binding protein; Ucp1 = uncoupling protein-1; Glut4 = facilitated glucose transporter-4; Fasn = fatty acid synthase, type-I) PMID: 29077876
  10. expressed in tail sebaceous glands; data do not support a thermoregulatory role PMID: 26914475
  11. UCP1 expression under inflammation is mediated by the increased expression of DBC1, which inhibits SIRT1 activity. PMID: 28481291
  12. MKK6 acts as a repressor of UCP1 expression, suggesting that its inhibition promotes adipose tissue browning and increases organismal energy expenditure. PMID: 29021624
  13. we find that rapamycin inhibits mTORC1 but not mTORC2, leading to suppression of elevated lipolysis and restoration of thermogenic protein UCP1 levels, respectively PMID: 27926859
  14. mTORC1 mediated many of the beneficial actions of FGF21 in vitro, including UCP1 and FGF21 induction, increased adiponectin secretion, and enhanced glucose uptake without any adverse effects on insulin action. PMID: 27681418
  15. Thus UCP1-dependent diet-induced thermogenesis limits obesity development during exposure to obesogenic diets but does not prevent obesity as such PMID: 28679625
  16. This study demonstrated that elimination of the gene expressing uncoupling protein-1 (UCP1), the enzyme responsible for thermogenesis, prevented musculoskeletal hyperalgesia in response to either a swim or BRL37344. PMID: 27437788
  17. Aortic UCP1 content was greater in females than males and its deletion improved ex vivo aortic vasomotor function in females only. Constitutive UCP1 content in BAT was similar between females and males and loss of UCP1 did not abolish sex differences in insulin sensitivity. Metabolic disruptions caused by UCP1 ablation did not appear to be contingent upon increased oxidative stress in mice under normal dietary conditions. PMID: 28655717
  18. Gelidium elegans stimulates the expression of PRDM16 and UCP-1 Protein in brown adipose tissue and suppresses hyperglycemia in high-fat diet mice. PMID: 28358328
  19. through interaction with Zfp516, LSD1 is recruited to UCP1 and other brown adipose tissue-enriched genes. PMID: 27264172
  20. Together the data show that both UCP1 and UCP3 play essential and complementary roles in thermogenic responses in the mouse and suggest that UCP3-dependent thermogenesis is an under-appreciated mode of thermogenic energy dissipation. PMID: 27647490
  21. in vitro experiments show that Arrdc3-null adipocytes responded to beta-adrenergic receptor agonist with decreased Ucp1 levels PMID: 28291835
  22. Despite the absence of UCP1 in the majority of epididymal beige adipocytes, these cells employ prominent creatine cycling as a UCP1-independent thermogenic mechanism. PMID: 28380374
  23. These novel findings uncover a previously unrecognized metabolic protective role of UCP1 that is independent of its already established role in energy homeostasis/ PMID: 27881400
  24. human and rodent Brown adipose tissue have similar UCP1 function per mitochondrion. PMID: 27508873
  25. Targeted mitochondrial uncoupling in adipose tissue and skeletal muscle of UCP1-transgenic mice increased substrate metabolism and ameliorates obesity. (Review) PMID: 27916644
  26. In the absence of regulators (fatty acids, retinoids), rodent UCP1 presents a high ohmic proton conductance that cannot be detected in human UCP1. PMID: 27750036
  27. The uncoupling of oxidative phosphorylation through a proton conductance pathway across the mitochondrial inner membrane is the mechanism for heat production in brown adipose tissue regulated by UCP1. (Review) PMID: 27621146
  28. Brown adipocytes trough their unique mitochondrial UCP1 protein burn glucose and lipids to perform thermogenesis in order to survive in cold environments. (Review) PMID: 27622583
  29. Brown and beige adipose tissues activate UCP1 protein in dissimilar fashion in response to cold temperature and depending on adipocytes differentiation state. PMID: 28109720
  30. ATGL activity is required for UCP1 activation in intact adipocytes. (Review) PMID: 27986537
  31. Metabolically inert perfluorinated fatty acids directly activate brown adipose tissue UCP1. PMID: 26041126
  32. Mice acclimatized to thermoneutrality revealed that Cpt2A-null interscapular brown adipose tissue failed to induce the expression of thermogenic genes such as Ucp1 and Pgc1a. PMID: 26854223
  33. Data show that fish oil intake increased oxygen consumption and rectal temperature, with concomitant upregulation of mitochondrial uncoupling protein 1 (UCP1) and beta3 adrenergic receptor (beta3AR). PMID: 26673120
  34. Skin temperature in the interscapular region of neonates was lower in uncoupling protein 1 knockout pups employed as a positive control, but not in Cox7a1 knockout pups PMID: 26635001
  35. PKA-dependent IRE1alpha-XBP1 activation is crucial for the transcriptional induction of Ucp1 in brown adipocytes. PMID: 26568450
  36. FGF21-mediated improvements in clearance of a glucose challenge require UCP1. PMID: 26586424
  37. antioxidant chemicals (such asN-acetylcysteine and Mn(III)tetrakis(4-benzoic acid)porphyrin chloride) and SB203580 (a known suppressor ofUcp1expression) decreasedUcp1and oxygen consumption inNrf2-deficient fibroblasts PMID: 26841864
  38. Data suggest beta-adrenergic activation of brown adipocytes leads to dissociation of Hdac1 from promoters of Ucp1/Pgc1a, to up-regulation of histone H3 acetylation, to dissociation of polycomb repressive complexes, and to up-regulation of thermogenesis. PMID: 26733201
  39. thermogenic mitochondrial reactive oxygen species alter the redox status of cysteine thiols in brown adipose tissue to drive increased respiration, and that Cys253 of UCP1 is a key target PMID: 27027295
  40. abundance decreased even more in cold-acclimated UCP1 knockout mice PMID: 26518386
  41. These data indicate that several important metabolic endpoints of FGF21 are UCP1 independent PMID: 25956583
  42. Data suggest that expression of Ucp1 in skeletal muscle can be regulated by dietary factors; the prebiotic epilactose prevents high-fat diet-induced obesity apparently via up-regulation of Ucp1 expression in skeletal muscles and brown adipose tissue. PMID: 26395755
  43. Data indicate that uncoupling protein 1 (UCP1) is required for the dietary methionine restriction (MR)-induced increase in energy expenditure (EE) but not insulin sensitivity. PMID: 25742717
  44. UCP1 and SLN are required to maintain optimal thermogenesis and loss of both systems compromises survival of mice under cold stress PMID: 25825499
  45. improved metabolism produced by oral administration of resveratrol is, at least in part, associated with increased thermogenesis followed by high expression of UCP1 and SIRT1 PMID: 24468941
  46. An experimental model of sleep apnea produced changes in uncoupling protein-1 expression and adiponectin levels. These results confirm previous findings on the response of brown adipose tissue to intermittent hypoxia. PMID: 24337908
  47. Data indicate that uncoupling protein 1 (UCP1) is not involved in control of reactive oxygen species production in brown-fat mitochondria. PMID: 24769119
  48. Zfp516 directly binds to the proximal region of the UCP1 promoter, not to the enhancer region where other transcription factors bind, and interacts with PRDM16 to activate the UCP1 promoter. PMID: 25578880
  49. We also found elevated mRNA levels of UCP-1 and Cidea after exposure to T3. PMID: 24771016
  50. Data indicate that the mobilization of free fatty acids (FFAs) was observed in interscapular brown adipose tissue (iBAT) of wild-type (WT) and uncoupling protein 1 (UCP1)-knockout mice. PMID: 24343897

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Subcellular Location Mitochondrion inner membrane, Multi-pass membrane protein
Protein Families Mitochondrial carrier (TC 2.A.29) family
Tissue Specificity Expressed in brown adipose tissue.
Database Links

KEGG: mmu:22227

STRING: 10090.ENSMUSP00000034146

UniGene: Mm.4177

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