Mouse Protein S100-A4(S100A4) ELISA kit

Instructions
Code CSB-EL020632MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name S100 calcium binding protein A4
Alternative Names S100a4 ELISA Kit; Capl ELISA Kit; Mts1 ELISA Kit; Protein S100-A4 ELISA Kit; Metastasin ELISA Kit; Metastatic cell protein ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein 18A2 ELISA Kit; Protein Mts1 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit
Abbreviation S100A4
Uniprot No. P07091
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %91-103
1:200Average %85
Range %80-90
1:400Average %103
Range %99-108
1:800Average %98
Range %94-105
Recovery
The recovery of mouse S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.418 2.524 2.471 2.277
20001.854 1.917 1.886 1.692
10001.360 1.294 1.327 1.133
5000.912 0.893 0.903 0.709
2500.659 0.627 0.643 0.449
1250.401 0.421 0.411 0.217
62.50.312 0.297 0.305 0.111
00.197 0.190 0.194
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

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