Mouse Protein S100-A4(S100A4) ELISA kit

Code CSB-EL020632MO
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07091
Lead Time 7-14 working days
Abbreviation S100A4
Target Name S100 calcium binding protein A4
Alias 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, OTTHUMP00000015469|OTTHUMP00000032895|S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)|S100 calcium-bind
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %91-103
1:200Average %85
Range %80-90
1:400Average %103
Range %99-108
1:800Average %98
Range %94-105
Recovery
The recovery of mouse S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.418 2.524 2.471 2.277
20001.854 1.917 1.886 1.692
10001.360 1.294 1.327 1.133
5000.912 0.893 0.903 0.709
2500.659 0.627 0.643 0.449
1250.401 0.421 0.411 0.217
62.50.312 0.297 0.305 0.111
00.197 0.190 0.194
References
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