Mouse Protein S100-A4(S100A4) ELISA kit

Code CSB-EL020632MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse S100A4 ELISA Kit was designed for the quantitative measurement of Mouse S100A4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name S100 calcium binding protein A4
Alternative Names S100a4 ELISA Kit; Capl ELISA Kit; Mts1 ELISA Kit; Protein S100-A4 ELISA Kit; Metastasin ELISA Kit; Metastatic cell protein ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein 18A2 ELISA Kit; Protein Mts1 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit
Abbreviation S100A4
Uniprot No. P07091
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %91-103
1:200Average %85
Range %80-90
1:400Average %103
Range %99-108
1:800Average %98
Range %94-105
Recovery
The recovery of mouse S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.418 2.524 2.471 2.277
20001.854 1.917 1.886 1.692
10001.360 1.294 1.327 1.133
5000.912 0.893 0.903 0.709
2500.659 0.627 0.643 0.449
1250.401 0.421 0.411 0.217
62.50.312 0.297 0.305 0.111
00.197 0.190 0.194
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9. Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis. Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels. Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes. In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors.
Gene References into Functions
  1. S100A4 knockout alone inhibits ATC cells (rich endogenous S100A4) survival and invasion, regardless of the BRAFV600E status, and potentiates the effect of vemurafenib on tumor regression in vitro and in vivo. PMID: 30196299
  2. Knockdown of S100A4 significantly reduced cardiac fibrosis and beta-catenin levels. PMID: 29758552
  3. High S100A4 expression is associated with Mesenchymal Transition in Glioblastoma. PMID: 28807938
  4. findings indicate that Idiopathic pulmonary fibrosis mesenchymal progenitor cells are intrinsically fibrogenic and that S100A4 confers mesenchymal progenitor cells with fibrogenicity. PMID: 28530639
  5. Data provide the first report on the in vivo role of S100a4 in intestinal tumourigenesis and describe a new role for S100a4 in the aetiology of desmoids formation. PMID: 27750112
  6. S100A4 expression protects cardiac myocytes against myocardial ischemia and is required for stabilization of cardiac function after MI. PMID: 27721024
  7. These findings provide further evidence on extracellular S100A4 as paracrine mediator of prometastatic endothelial dysfunction involving its interaction with RAGE. PMID: 27387233
  8. this study has demonstrated a crucial role for retinoic acid in promoting IL-22 production and tempering dendritic cell function through downregulating S100A4 protein during viral hepatitis PMID: 28363907
  9. Extracellular S100A4 is important for the regulation of bone formation by activating the NF-kappaB signaling pathway in osteoblasts. PMID: 27998393
  10. Interaction of extracellular S100A4 with RAGE prompts prometastatic activation of melanoma cells. PMID: 26928771
  11. Our results indicated that RNV was ameliorated by Ad-S100A4-RNAi transfer in a mouse model of OIR through mediation of the anti-apoptotic effect of Bcl-2 by reducing the expression of CREB. PMID: 26358273
  12. The thymic CD45-FSP1+ cells are a subpopulation of fibroblasts, which is crucial for the maintenance and regeneration of thymic epithelial cells. PMID: 26445893
  13. Enhanced S100A4 expression was observed in remodeled intrapulmonary arteries of mice with smoke-induced emphysema. PMID: 26483185
  14. Downregulation of AKT3 increases migration and metastasis in triple negative breast cancer cells by upregulating S100A4. PMID: 26741489
  15. S100A4 promotes liver fibrosis by activating HSCs, which may represent a potential target for anti-fibrotic therapies. PMID: 25111176
  16. The S100A4 blocking antibody (6B12) reduces tumor growth and metastasis in a model of spontaneous breast cancer. PMID: 25884510
  17. Functional studies of human TH2 cells as well as mouse models of allergy showed that deletion of one of the genes, S100A4, resulted in decreased signs of allergy. PMID: 24401939
  18. S100A4 in endothelial cells is involved in tube formation, and suggest its potential as a molecular target for inhibiting tumor angiogenesis PMID: 23929008
  19. S100A4 is overexpressed in an experimental head and neck squamous cell carcinoma, and its inhibition reduces tumor burden. PMID: 24037664
  20. interaction of macrophage-derived S100A4 with B16-F10 melanoma results in increased metastatic lung colonization in vivo PMID: 24613382
  21. S100A4 is a regulator of bone formation, which inhibits bone excess in the estrogen-sufficient mice and prevents the cortical bone loss in the estrogen-deprived mice. PMID: 23798573
  22. the role of S100A4 in bone homeostasis, was analysed. PMID: 23830916
  23. FSP1 is not a specific marker for fibroblasts in cardiac remodeling and fibrosis. PMID: 23997102
  24. The S100A4 protein, mostly studied in cancer, is overexpressed in the damaged human and rodent brain and released from stressed astrocytes. PMID: 23149742
  25. S100A4/Mts1 over-expression combined with female gender is permissive to the development of experimental pulmonary arterial hypertension in mice PMID: 22185646
  26. Bone marrow-derived FSP-1(+) cells enhance neointima formation through an increase in transendothelial invasion with stimulation of smooth muscle cell proliferation. FSP-1 silencing in bone marrow cells suppresses neointima formation in vein graft. PMID: 22116816
  27. S100A4 in the peripheral circulation may play a role in the induction of blood-brain barrier disruption under the inflammation associated with rheumatoid arthritis. PMID: 21627981
  28. Data show that reduction in metastasis due to the loss of S100A4(+) fibroblasts correlated with a concomitant decrease in the expression of several ECM molecules and growth factors, particularly Tenascin-C and VEGF-A. PMID: 21911392
  29. A lack of S100A4 results in mobilization of systemic inflammation, more efficient bacterial clearance, and increased protection against joint destruction caused by staphylococcal arthritis. PMID: 21844298
  30. causative role of S100A4 in osteosarcoma lung metastasis shown in the murine xenograft model PMID: 21360024
  31. demonstrate pro-metastatic function of S100A4 in the tumor microenvironment and define how the tumor cell-derived cytokine RANTES acts as a critical regulator of S100A4-dependent tumor cell dissemination. PMID: 20442771
  32. role of S100A4 in normal mammary gland, its spatial and temporal expression patterns and possible function in branching morphogenesis PMID: 21195708
  33. FSP1 is a marker of a specific subset of inflammatory macrophages in liver injury, fibrosis, and cancer. PMID: 21173249
  34. These studies establish S100A4 as a regulator of physiological macrophage motility and demonstrate that S100A4 mediates macrophage recruitment and chemotaxis in vivo. PMID: 20519440
  35. S100A4 activates NF-kappaB by inducing phosphorylation of IKKalpha/beta, leading to increased IkappaBalpha phosphorylation in osteosarcoma cells. PMID: 20507646
  36. Data show that in S100A4(-/-) PyMT tumors, a significant suppression of T-cell infiltration was found at the transition period. PMID: 20103644
  37. Calcium coordination studies of the metastatic Mts1 protein PMID: 11914069
  38. the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
  39. Sequence-specific chemical shift assignment of calcium-loaded murine S100A4 PMID: 12153041
  40. role as negative regulator of mineralization and osteoblast differentiation PMID: 12619934
  41. metastasis-inducing S100A4 protein plays a pivotal role in the tumor-stroma environment PMID: 15047714
  42. These data indicate that S100A4 reduces the migratory capacity of reactive white matter astrocytes in the injured CNS and is involved in glial scar formation after injury. PMID: 16463066
  43. S100A4 expression was many -fold greater in the papilla as compared with the cortex and increased further in the papilla upon 36 h of thirsting in kidney in response to hypertonic stress PMID: 17200116
  44. Overexpression of S100A4 protein is associated with liver metastasis in pancreatic cancer PMID: 17786304
  45. Enhanced generation of elastin peptides in S100A4/Mts1 mice may promote increased viral entry in the vessel wall. PMID: 18083765
  46. comparison of secretion of S100A4 from tumor cells & lymphocytes; injection of antibodies to S100A4 to mice with transplanted M3 melanoma inhibited tumor growth suggesting S100A4 production protects tumor cells from cytotoxicity of immunocompetent cells PMID: 19024009

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Subcellular Location Secreted. Nucleus. Cytoplasm.
Protein Families S-100 family
Tissue Specificity Specifically expressed in different metastatic cells.
Database Links

KEGG: mmu:20198

STRING: 10090.ENSMUSP00000001046

UniGene: Mm.3925

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