Mouse Protein Wnt-2(WNT2) ELISA kit

Code CSB-EL026133MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
wingless-type MMTV integration site family member 2
Alternative Names
Wnt2 ELISA Kit; Irp ELISA Kit; Wnt-2 ELISA Kit; Protein Wnt-2 ELISA Kit; INT-1-related protein ELISA Kit; IRP ELISA Kit
Abbreviation
WNT2
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %83-87
1:2Average %94
Range %89-98
1:4Average %103
Range %97-108
1:8Average %99
Range %95-104
Recovery
The recovery of mouse WNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-101
EDTA plasma (n=4)8983-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.315 2.501 2.408 2.247
5001.478 1.589 1.534 1.373
2500.928 0.984 0.956 0.795
1250.603 0.587 0.595 0.434
62.50.325 0.340 0.333 0.172
31.250.257 0.277 0.267 0.106
15.60.201 0.215 0.208 0.047
00.157 0.164 0.161
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse WNT2 ELISA Kit was designed for the quantitative measurement of Mouse WNT2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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 Q&A
Q:

a. The active ingredient of the stop solution and its concentration ?
b. The type, concentration, and state (liquid/lyophilized) of the preservatives used. Please name the components with preservatives ?
c. If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
d. Expression host of the standards ?
e. Which components have H202 ( including other forms of peroxide such as urea hydrogen peroxide) ?Please list with the concentration of H202/peroxide in each, if any?

A:
Thanks for your inquiry!
CSB-EL026133MO
Stop solution is 4N sulfuric acid.
Preservatives is liquid proclin 300(1:3000);
Standard is NS0-derived recombinant protein. It is lyophilized powder.
The concentration after reconstitution is 1000pg/mL(pls refer to Page 9 in the manual);
The kit doesn't contain H2O2.
Pls let me know if you have any further questions. Thank you.
Q:

Just to clarify, Preservatives (proclin 300) is included only in the lyophilized standard ?
Also, please confirm that the only preservative is proclin 300? If not, please let me know the names and concentrations

A:
All components contain preservatives (proclin 300) except wash buffer、TMB Substrate and Stop Solution.

Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.
Gene References into Functions
  1. Transient expression of WNT2 promotes somatic cell reprogramming by inducing beta-catenin nuclear accumulation. PMID: 27211212
  2. Study indicates essential roles for Wnt2 and Wnt3 in chronic restraint stress-induced depression-like behaviors and antidepressant. PMID: 27622936
  3. Wnt 2 levels were markedly down-regulated by retinoic acid in pancreatic stellar cells and chronic pancreatitis. PMID: 26556479
  4. We propose that the WNT2/CTNNB1 pathway regulates CX43 expression and gap junction intercellular communication in granulosa cells by modulating CTNNB1 stability and localization in adherens junctions PMID: 23843235
  5. Pdgf signaling potentiates Wnt2-Wnt7b signaling to promote high levels of Wnt activity in mesenchymal progenitors that is required for proper development of endoderm-derived organs, such as the lung PMID: 22949635
  6. Expression of WNT2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. PMID: 22763454
  7. Wnt2 plays strong positive stage-specific role in cardiogenesis through non-canonical Wnt pathway in murine ES cells. PMID: 22146296
  8. show that Wnt2 signaling is necessary and sufficient for activation of a transcriptional and signaling network critical for smooth muscle specification and differentiation including myocardin/Mrtf-B and the signaling factor Fgf10 PMID: 21704027
  9. Wnt2 expression is downregulated at lung bud stage in the nitrofen model. Optical projection tomography is potentially a useful approach to visualize both gene expression and morphology during very early stages of lung development. PMID: 21034933
  10. The canonical WNT signaling pathway is intact in mouse granulosa cells and that WNT2 can act through beta-catenin, likely downstream of FZD9, to regulate DNA synthesis. PMID: 20107203
  11. Results identify Wnt2 as a novel regulator of dopaminergic progenitors and dopaminergic neuron development. PMID: 20018874
  12. Wnt2 works in a feedforward transcriptional loop with Gata6 to regulate posterior cardiac development. PMID: 20159597
  13. Upregulation by GATA6 in differentiating embryonal carcinoma cells is mediated by the direct binding of GATA-factor(s) to the Wnt2 promoter, suggesting Wnt2 is an immediate downstream target of GATA-factor regulation during early cardiogenesis. PMID: 16621466
  14. Wnt2 coordinates the commitment of mesoderm to hematopoietic, endothelial, and cardiac lineages in embryoid bodies PMID: 17098737
  15. FMR1, MECP2, and WNT2 expression were drastically down regulated in the Fragile X mouse brain. PMID: 19138667
  16. Study revealed new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. PMID: 19185060
  17. Data reveal that canonical Wnt2/2b signaling is required for the specification of lung endoderm progenitors in the developing foregut. PMID: 19686689

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Tissue Specificity
In embryos in the developing allantois, pericardium heart, and ventral-lateral mesoderm; in adults in lung, brain, heart and placenta.
Database Links
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