Mouse phosphoenolpyruvate carboxykinase,PCK ELISA Kit

Instructions
Code CSB-E13613m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name phosphoenolpyruvate carboxykinase 1 (soluble)
Alternative Names Pck1 ELISA Kit; Pepck ELISA Kit; Phosphoenolpyruvate carboxykinase ELISA Kit; cytosolic [GTP] ELISA Kit; PEPCK-C ELISA Kit; EC 4.1.1.32 ELISA Kit
Abbreviation PCK1
Uniprot No. Q9Z2V4
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 96    
Range % 93-99    
1:200 Average % 88    
Range % 84-93    
1:400 Average % 101    
Range % 97-106    
1:800 Average % 85    
Range % 80-91    
Recovery
The recovery of mouse PCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 103 100-105    
EDTA plasma (n=4) 89 85-94    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
100 2.036 2.101 2.069 1.875    
50 1.631 1.602 1.617 1.423    
25 1.108 1.201 1.155 0.961    
12.5 0.789 0.760 0.775 0.581    
6.25 0.485 0.491 0.488 0.294    
3.12 0.345 0.339 0.342 0.148    
1.56 0.275 0.281 0.278 0.084    
0 0.191 0.196 0.194      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Gene References into Functions
  1. Data (including data from studies in knockout mice) suggest that Pck1 in liver plays role in glucose homeostasis; here, Pck1 deficiency (in knockout mice) can be successfully treated by re-expression of Pck1 in liver, thus partially rescuing pups from fatal neonatal hypoglycemia. Pck1 appears to play roles in metabolism in lung and skeletal muscle. PMID: 27785616
  2. Silencing PCK1 or inhibiting its enzymatic activity slows the growth of tumor-repopulating cells in vitro and impedes tumorigenesis in vivo. PMID: 25712344
  3. PEPCK-M expression partially rescued defects in lipid metabolism, gluconeogenesis and TCA cycle function impaired by PEPCK-C deletion, while approximately 10% re-expression of PEPCK-C normalized most parameters. PMID: 23466304
  4. FAT/CD36 regulates PEPCK in adipose tissue and that this could be secondary to reductions in lipolysis. PMID: 23302781
  5. PCB 126 blunted the forskolin-stimulated increase in phosphoenolpyruvate carboxykinase (PEPCK) mRNA levels. PMID: 22615911
  6. there are multiple cytokine pathways by which inflammation inhibits PEPCK expression in adipose tissue which could contribute to the increased mobilization of fatty acids during inflammation PMID: 21450790
  7. Glucagon can also regulate Pck1 in the intestine, but not in the kidneys. PMID: 22013018
  8. Pck1 plays an important role in both the mammary gland adipocytes and epithelial cells during lactation PMID: 21504969
  9. Results suggest that HDAC3 mediates NF-kappaB activity to repress Pepck transcription. PMID: 21406501
  10. Results show the effect of miR-29a in counteracting insulin action on PEPCK gene expression by primarily targeting PI3K and abrogating downstream insulin signaling in HepG2 cells. PMID: 20943204
  11. PPARgamma binding site in the promoter of Pck1 is essential for maintenance of lipid metabolism and glucose homeostasis and disease prevention. PMID: 20124556
  12. hyperhomocysteinemia may be considered as a risk factor that contributes to the development of insulin resistance with respect to elevated glucose output and upregulation of PEPCK, probably via the PKA pathway PMID: 20011977
  13. Data show that gamma-linoleic acid positively modulates TR4 activity to promote the expression of downstream genes apolipoprotein E (ApoE) and phosphoenolpyruvate carboxykinase. PMID: 19800043
  14. ATF3 bound to the ATF/cAMP-responsvie element site derived from the promoter of the gene encoding the gluconeogenic enzyme phosphoenolpyruvate carboxykinase PMID: 11916968
  15. The binding of nuclear receptors to PEPCK RARE1, RARE2 and RARE3 indicates a complex pattern of retinoid receptor and orphan nuclear receptor binding. PMID: 12354671
  16. Liver-specific PEPCK knockout mice are able to maintain normal blood sugar while fasting by increasing gluconeogenesis and by glucose metabolism PMID: 12829628
  17. Reduction in RNA Pol II association is indicative of interruption in direct interactions of RNA Pol II with PEPCK promoter, with general transcription factors and/or with coregulator molecules contributeing to activation of PEPCK gene. PMID: 14652357
  18. PEPCK has a role in tricarboxylic acid cycle activity in mouse liver PMID: 15347677
  19. regulation of glyceroneogenesis and of PEPCK-C gene expression and activity by FAs, retinoic acids, glucocorticoids and the hypolipidemic class of drugs, thiazolidinediones. PMID: 15733733
  20. MKP-3 activates PEPCK gene transcription and increases gluconeogenesis PMID: 16126724
  21. ERRalpha acts as a transcriptional repressor of the PEPCK gene PMID: 16267049
  22. Nuclear receptors bind to retinoic acid response elements (RAREs) in the PEPCK promoter in liver. PMID: 16317119
  23. HNF-4alpha, RXR alpha, RAR alpha, PPAR alpha & chicken ovalbumin upstream promoter transcription factor II bind to the downstream retinoic acid response unit RARE1/RARE2, & PPARalpha & RXRalpha bind to the upstream RARE3 of the PEPCK gene. PMID: 16713227
  24. growth hormone corrects PEPCK expression in aging livers PMID: 17107955
  25. PEPCK expression is coordinate with hepatic energy metabolism to control gluconeogenesis. PMID: 17403375
  26. IFN-gamma exerts a tissue-specific action in rodents and humans, having glyceroneogenesis and the PEPCK-C gene as selective targets to intensify fatty acid release from adipocytes PMID: 17495004
  27. PEPCK-C(mus) mice had an extended life span relative to control animals PMID: 17716967
  28. Data show that knocking down hepatic PEPCK-C resulted in improved glycemia and insulinemia, lower triglyceride, and higher total and HDL cholesterol, and improved insulin signaling in the liver, muscle, and adipose tissue. PMID: 18443203
  29. Reduced insulin sensitivity in fat-fed H-AR(-/y) mice was associated with decreased phosphoinositide-3 kinase activity and increased phosphenolpyruvate carboxykinase expression and correlated with increased protein-tyrosine phosphatase 1B expression. PMID: 18449947
  30. The effects of several variants of retinoic acid on the expression and activity of PEPCK in human, mouse, and rat adipose tissue are reported. PMID: 18492826
  31. Reduced CREB phosphorylation (Ser-129) associated with inactivation of GSK3beta by Ser-9 phosphorylation may be the major mechanism underlying PEPCK-C gene suppression by AMPK-activating agents such as biguanide PMID: 18801732
  32. In vivo phosphoenolpyruvate carboxykinase promoter mapping identifies disrupted hormonal synergism as a target of inflammation during sepsis in mice. PMID: 19821526

Show More

Hide All

Subcellular Location Cytoplasm
Protein Families Phosphoenolpyruvate carboxykinase [GTP] family
Database Links

KEGG: mmu:18534

STRING: 10090.ENSMUSP00000029017

UniGene: Mm.266867

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved. 鄂ICP备15011166号-1