Mouse stromal cell derived factor 1α(SDF-1α/SDF1A)ELISA kit

Instructions
Code CSB-EQ027494MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name stromal cell derived factor 1α(SDF-1α/SDF1A)
Alternative Names Cxcl12 ELISA Kit; Sdf1 ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; SDF-1 ELISA Kit; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; TPAR1 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; PBSF ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; TLSF ELISA Kit
Abbreviation SDF1A/CXCL12
Uniprot No. P40224
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %101
Range %99-105
1:2Average %96
Range %91-98
1:4Average %89
Range %84-94
1:8Average %98
Range %95-104
Recovery
The recovery of mouse SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9895-101
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.358 2.467 2.413 2.258
52.002 1.977 1.990 1.835
2.51.355 1.425 1.390 1.235
1.250.815 0.858 0.837 0.682
0.6250.510 0.525 0.518 0.363
0.3120.345 0.373 0.359 0.204
0.1560.271 0.280 0.276 0.121
00.156 0.153 0.155
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells (By similarity). Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Gene References into Functions
  1. superparamagnetic iron oxide nanoparticles also stimulated CXCR4 (C-X-C chemokine receptor type 4) expression and CXCR4-SDF-1 (Stromal cell-derived factor 1) signaling in mesenchymal stem cells. PMID: 29734748
  2. This study regarding high SDF-1 levels in our mouse model of ototoxicity would play a major role in the development of therapeutic agents using MSC homing. PMID: 29430461
  3. CXCL12-CXCR4 signalling is essential for the correct patterning of aortic arches and pulmonary arteries during development. PMID: 29016745
  4. Data (including data from studies in knockout mice) suggest that adipocyte autocrine function involving Sdf1 regulates insulin resistance; Sdf1 gene expression correlates with insulin-desensitized conditions in adipocytes but not other tissues (liver, skeletal muscle); adipocyte-specific ablation of Sdf1 enhances insulin sensitivity in adipose tissues and in whole body. PMID: 29581126
  5. postnatal CXCL12 signaling promotes a specific interneuron circuit that inhibits medial prefrontal cortex activity PMID: 27497284
  6. Study reports that stromal cell-derived factor-1alpha elevated or therapeutically administered in ischemic wounded tissue can stimulate both local endothelial cells (EC) and bone marrow-derived endothelial progenitor cells (EPC) to express reciprocally E-selectin/ligand pairs and thereby enhance EPC-EC interactions. PMID: 27713493
  7. suggest that miR-155 modulates SHIP-1 expression that subsequently affects CXCL12-CXCR4 signaling axis via Akt activation PMID: 28174416
  8. Authors produced recombinant CXCL12 and CXCL12(5-67) and evaluated their effect in murine adult NSCs migration and survival in vitro. We showed CXCL12(5-67) does not promote NSCs migration, but does induce cell death. PMID: 28623786
  9. Study demonstrates that CXCR4/CXCL12 axis can limit oxidative stress injury in hematopoietic stem cells (HSCs) by reducing mitochondrial oxidative stress. CXCL12 has a direct rescue effect on oxidative stress-induced HSC damage at the mitochondrial level. PMID: 27886253
  10. a defect of CXCL12 promoter histone acetylation may represent an additional process participating in CXCL12 expression extinction in colon cancer PMID: 28418886
  11. The structure of murine germinal centers (GC) and the localization of GC B cells are impaired when CXCL12 is unable to bind to cellular or extracellular surfaces. PMID: 28193885
  12. These findings indicate that the CXCL12alpha-CXCR4 axis plays an important role in the regeneration of the neuromuscular junction after motor axon injury. PMID: 28559442
  13. Here, we show that cabozantinib rapidly eradicates invasive, poorly differentiated PTEN/p53-deficient murine prostate cancer. This was associated with enhanced release of neutrophil chemotactic factors from tumor cells, including CXCL12 and HMGB1, resulting in robust infiltration of neutrophils into the tumor. PMID: 28274958
  14. nitration on Tyr7 under inflammatory conditions is a novel natural posttranslational regulatory mechanism of CXCL12 which may downregulate the CXCR4-mediated inflammatory and tumor-promoting activities of CXCL12 PMID: 27566567
  15. SDF-1 is secreted shortly after UPEC infection initiating immune cell accumulation. PMID: 28683322
  16. The data suggest that SDF-1beta provides synergistic effects supporting BMP-2-induced, BMSC-mediated bone formation and appears suitable for optimization of bone augmentation in combination therapy protocols. PMID: 26227988
  17. Hyaluronic acid-laminin hydrogels increase neural stem cell transplant retention and migratory response to SDF-1alpha in a manner critically dependent on signaling via the SDF-1alpha-CXCR4 axis. PMID: 27645115
  18. The Function of SDF-1-CXCR4 Axis in SP Cells-Mediated Protective Role for Renal Ischemia/Reperfusion Injury by SHH/GLI1-ABCG2 Pathway PMID: 27454381
  19. Adora2B stimulation promotes FGF2 and CXCL12 expression in FAP-positive melanoma-associated fibroblasts, contributing to the creation of a tumor-promoting microenvironment. PMID: 27590504
  20. CXCL12 in cardiomyocytes is not involved in cardiac development. * CXCL12 deficiency in cardiomyocytes improves outcome of myocardial infarction. * CXCL12 overexpression in cardiomyocytes worsens outcome of myocardial infarction. * CXCL12 increases fibrosis and invasion of Th1 cells in the heart after infarction. PMID: 27251706
  21. findings suggest that PECAM-1 enhances SDF-1-induced chemotaxis by augmenting and prolonging activation of the PI3K/Akt/mTORC1 pathway and Rap1 and that PECAM-1, at least partly, exerts its activity by inhibiting SDF-1-induced internalization of CXCR4 PMID: 28974577
  22. endothelial CXCR7+ cells regulate CXCL12 gradient direction by controlling concentrations near but not far from the vasculature. PMID: 29117251
  23. This study showed that release of BMP-2 and SDF-1alpha from heparinized MCM scaffolds allows for the reduction of the applied BMP-2 concentration since SDF-1alpha seems to enhance the osteoinductive potential of BMP-2. PMID: 27060915
  24. The results of this study suggested that enhanced interaction between STAT3 and p300 mediated the epigenetic upregulation of CXCL12 in dorsal horn neurons, which contributed to the antitubulin chemotherapeutics-induced persistent pain PMID: 28072604
  25. Dipeptidyl peptidase-4 inhibition, independent of glucagon-like peptide-1 receptor signaling, contributes to protection of the diabetic kidney through SDF-1-dependent antioxidative and antifibrotic effects and amelioration of adverse renal hemodynamics. PMID: 27475229
  26. High Cxcl12 expression is associated with Prostate Cancer. PMID: 28687617
  27. Authors demonstrate that targeting the SDF-1/CXCR4 pathway in the context of KLF10 deletion substantially suppresses PDAC progression PMID: 28581520
  28. Adipocytes promoted osteoclast differentiation, function and expression of adhesion-related molecules through the CXCL12/CXCR4 signalling pathway. PMID: 27868262
  29. these findings demonstrate that expression of Hmga2 cooperates with Jak2(V617F) in the pathogenesis of Mmyelofibrosis. PMID: 28637665
  30. Data demonstrated that sustained expression of CXCL12 by MSCs in the primary tumour site inhibits metastasis through reduction of CXCR7, while, in the presence of TGFbeta, this CXCL12 effect of MSCs on tumour cells is relieved. PMID: 27669436
  31. CXCL12 upregulation prior to stroke onset, and its actions following stroke, contribute to the endogenous, anti-inflammatory phenotype induced by repetitive hypoxic preconditioning PMID: 27006446
  32. Results suggest that SDF-1/CXCR4 signaling plays an important role in the dynamics associated with adult sub-ventricular zone lineage cell proliferation and differentiation. PMID: 27288704
  33. TNF plays an inhibitory role in modulating myocardial SDF-1 production and blockade of TNF signaling by ablation of TNFR1 and TNFR2 genes increased SDF-1 expression in the heart. These data expand on TNF signaling-initiated mechanisms in myocardium, which may lend a more complete understanding of SDF-1 and TNFR-derived actions in hopes of advancing ischemic heart injury treatments. PMID: 27979472
  34. CD26-cleavage skews CXCL12 towards beta-arrestin dependent recruitment through ACKR3 and destroys the CXCR4-mediated lymphocyte chemoattractant properties of CXCL12 in vivo PMID: 28322746
  35. these data highlight AnxA1 as a novel determinant of neutrophil maturation and the mechanisms behind blood neutrophil homing to BM via the CXCL12/CXCR4 pathway. PMID: 26892496
  36. CXCL12/CXCR4 regulates HA and LG following corneal suture placement, and provides a novel strategy to inhibit LG. Notably, the present study is the first to demonstrate evidence that CXCL12/CXCR4 modulates LG in a corneal suture-induced mouse model. PMID: 27121088
  37. the present study indicates that the CXCL12/CXCR4 signaling pathway is important during the development of cochleae in neonatal mice. PMID: 27052602
  38. crosstalk between astrocytic CXCL12 and microglial CXCR4 in the pathogenesis of neuropathic pain. PMID: 27030717
  39. Overexpression of SDF-1alpha could chemotaxize endothelial progenitor cells to reach local wounds, thus further accelerating angiogenesis in the transplant site PMID: 25853481
  40. A model of SDF-1 regulation in the hypoxia pathway was constructed; the underlying mechanisms of SDF-1 kinetics and a novel incoherent feed forward loop regulating SDF-1 expression were proposed. PMID: 26701884
  41. We showed that CXCL12, a potent chemoattractant for CXCR4-expressing NSPCs, was upregulated in the ischemic lesion of N-PRbeta-KO mice. PMID: 26435273
  42. DPP-4 inhibition may have direct protective effects on the post-myocardial infarction heart by inducing an antiapoptotic effect and inhibiting a decrease in vessel number through the SDF-1a/CXCR4-mediated STAT3 signaling pathway. PMID: 26739213
  43. findings demonstrate that Twist-1, which maintains BMSC at an immature state, endows them with an increased capacity for supporting hematopoiesis via direct activation of CXCL12 gene expression. PMID: 26718114
  44. confirm a pivotal role of the SDF-1/CXCR4/CXCR7 axis for chronic allograft vasculopathy development PMID: 26265085
  45. Time-dependent changes in endometrial hypoxia during menstruation correlated with the regulation of mRNAs encoding for the angiogenic genes Vegfa and Cxcl12. PMID: 26780953
  46. The results of this study findings the post-CNS-inflammation role of CXCL12 in augmenting the endogenous myelin/neuronal repair capacity in MS-like disease, likely via CXCL12/CXCR4 autocrine signaling. PMID: 26747276
  47. CXCR4/CXCL12 signaling may control movement of epithelial progenitors from the dental stem cell niche. PMID: 26246398
  48. our present study provided evidence that SDF-1 mediated CSCs migration through CXCR4 and CXCR7 via MEK/ERK and PI3K/Akt pathway PMID: 26578388
  49. Following fracture, a CXCL12(+)-BMP2(+) perivascular cell population is recruited along the endosteum. PMID: 25967044
  50. Data suggest that the conditional chemokine CXCL12 line can be used as a powerful tool to manipulate CXCL12 signaling and function in vivo. PMID: 26505253
  51. SDF-1 expressed by liver sinusoidal endothelial cells can be a major player in the recruitment of hematopoietic stem and progenitor cells to the liver during extramedullary hematopoiesis induced by hematopoietic mobilizers PMID: 26093947
  52. EMMPRIN promotes tumor growth and metastasis by recruitment of bone marrow-derived cells through controlling secretion and paracrine signaling of SDF-1 and VEGF. PMID: 26416452
  53. Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation PMID: 26214286
  54. Independent of the reduction of blood pressure, aliskiren enhanced ischemia-induced neovasculogenesis in a dose-dependent manner via VEGF/SDF-1alpha related mechanisms in diabetic mice. PMID: 26305217
  55. The estradiol-CXCL12/CXCR4 signaling pathway may be useful in determining treatments for endometrial disorders, and may be antagonized to block stem cell migration to endometriosis. PMID: 25957946
  56. The SDF-1/CXCR4 system was implicated as contributing to the changes in these stem cell populations upon bone injury. PMID: 26530150
  57. Both CXCR7 and Rac1 are required for extracellular signal-regulated kinases (ERK) 1/2 activation and subsequent NPC migration, indicating that CXCR7 could serve as a functional receptor in CXCL12-mediated NPC migration independent of CXCR4. PMID: 25833331
  58. hepatic expression of the inflammatory CXC chemokine ligands (CXCL)9 and CXCL10 strongly increased whereas homeostatic CXCL12 significantly decreased. PMID: 26052942
  59. Src family kinases are activated by SDF-1/CXCR4 signaling and play an essential role in SDF-1/CXCR4-mediated bone marrow progenitor cells chemotactic response and ischemic cardiac recruitment. PMID: 25655934
  60. findings establish CRCs as a major stromal cell type in the GC DZ and suggest that CRCs support critical activities of GC B cells in the DZ niche through Cxcl12 expression and direct cell-cell interactions. PMID: 26453751
  61. These findings imply that sequence-controlled application of SDF-1 and BMP-2 must be further investigated for the enhancement of robust osteogenesis in bone defects. PMID: 25781922
  62. Cultured principal cells exhibited an HIF1alpha-dependent increase of Sdf1 transcription in response to media acidification. PMID: 26517693
  63. This study demonstrate that intermediate progenitors release the chemotactic cytokine CXCL12 to promote intracortical interneuron migration and growth of thalamic axons via the cognate receptor CXCR4. PMID: 26400936
  64. These results suggest that reconstitution of renal endothelium post-ischemia partially depends on a paracrine loop of SDF1-CXCR4 between resident endothelium and bone marrow-derived cells. PMID: 25833353
  65. SDF-1 upregulation may be an important macrophage effector mechanism during I/R injury. PMID: 25478952
  66. CXCL12 promotes alveolar epithelial cell migration by binding to its receptor CXCR4 and may have a role in lung healing. PMID: 26212341
  67. B cells from Sdc4(-/-) mice showed reduced chemotactic migration toward stromal cell-derived factor 1 (SDF-1) and reduced SDF-1-mediated Akt phosphorylation. PMID: 25989265
  68. increased number of mesenchymal stem cells (MSCs) was found in peripheral blood after SDF-1alpha treatment PMID: 26145601
  69. demonstration of the importance of the CXCR4/SDF-1 axis for the pectoral girdle muscle formation in avians and mammals PMID: 24972797
  70. The tumor microenvironment interaction between ANXA2-CXCL12 is critical for metastatic phenotypes and may impact chemotherapeutic potential. PMID: 25139998
  71. T-ALL cells are in direct, stable contact with CXCL12-producing bone marrow stroma. Cxcl12 deletion from vascular endothelial, but not perivascular, cells impeded tumor growth, suggesting a vascular niche for T-ALL. PMID: 26058075
  72. describe SMAD-dependent BMP signaling as a novel regulator of CXCL12 production in the bone marrow niche, influencing hematopoietic stem/progenitor cell homing, engraftment, and mobilization PMID: 25069965
  73. The results indicate that, through Gi and JAK1 and 2 kinases activation, CXCL12 signaling cooperates to build the immune synapse and to maintain adhesive contacts between Antigen-Presenting Cells and T cells, required for continuous TCR signaling. PMID: 25917087
  74. the spermatogonial population may not only be simply controlled by interaction of Cxcl12 with Cxcr4 but may also involve Cxcr7 as an important player PMID: 25460567
  75. Loss of Cxcl12 is associated with severe defect in Coronary Artery Development. PMID: 26017770
  76. Loss of Cxcl12 is associated with selective failure in arterial maturation. PMID: 26017771
  77. an EPC SDF-1/CXCR4 axis plays an important role in bone fracture healing using Tie2-Cre(ER) CXCR4 conditional knockout mice. PMID: 25130304
  78. C/EBPbeta mediates osteoclast recruitment by regulating endothelial progenitor cell expression of SDF-1alpha. PMID: 24618682
  79. platelets trigger lung regeneration by supplying stromal-cell-derived factor-1. PMID: 25621952
  80. SDF-1-dependent neutrophil recruitment to the liver is crucial for TIMP-1-induced premetastatic niche formation. PMID: 25131778
  81. our findings support a model in which MMP-10 activity modulates CXCR4/SDF1 signaling, which is essential for efficient skeletal muscle regeneration. PMID: 24548137
  82. effects of pre-irradiation and SDF-1 suppression on the progression of murine astrocytoma cells grown in different stromal beds PMID: 24937369
  83. results reveal an important role of the SDF-1/CXCR4 axis in skin inflammation and inflammatory angiogenesis PMID: 24695674
  84. In this study we found that CXCL12 is an adipocyte-derived chemotactic factor that recruits macrophages, and that it is a required factor for the establishment of obesity-induced adipose tissue inflammation and systemic insulin resistance. PMID: 24744121
  85. The results represent the first evidence that SDF-1/CXCR4 signaling mediates acute cardioprotection through modulating beta-adrenergic receptor signaling in vivo. PMID: 24646609
  86. Activated macrophages inactivate CXCL12, the major chemokine for endothelial progenitor cell recruitment during wound healing. PMID: 25211042
  87. hyaluronan exacerbates CNS autoimmunity, enhances encephalitogenic T-cell responses, and suppresses the protective chemokine CXCL12 in CNS tissue. PMID: 24973214
  88. differential and hierarchically ordered roles for CXCR4/CXCL12- vs. CXCR7/CXCL12-dependent effects during experimental autoimmune neuritis PMID: 23452257
  89. findings indicate CXCL12 secreted from glioma stem cells (autocrine/paracrine CXCL12) regulates their proliferation, but probably not angiogenesis PMID: 24442483
  90. demonstrate that IBE-elicited signals increase SDF-1 expression through the CXCR2/miR-223/miR-27b pathway in C6 astrocytoma cells and primary astrocytes PMID: 24999035
  91. results demonstrated that SDF-1alpha and laminin-based ECM (Matrigel and laminin) significantly and synergistically enhanced NPSC migration and acute neuronal differentiation PMID: 24438907
  92. SDF-1 regulates the spatial distribution of Megakaryocytes in the marrow and consequently circulating platelet numbers. PMID: 24735964
  93. IKKbeta and IKKalpha are simultaneously essential for cell migration in response to CXCL12 alone. PMID: 24747690
  94. SDF-1alpha signalling, via CXCR4 activation, up-regulated c-kit expression by inhibiting DNMT1 and DNMT3beta expression and global DNMT activity, and by subsequent demethylation of the c-kit gene. PMID: 23891309
  95. to egress from bone marrow , HSPCs first have to be released from bone marrow niches by blocking the SDF-1-CXCR4 retention signal. PMID: 24490172
  96. The unique and non-overlapping patterns of CXCL12 and CXCL14 expression in ocular tissues suggest that these two chemokines may interact and have important functions in cell proliferation, differentiation and migration during eye development. PMID: 23727298
  97. results revealed iron deficiency induced expression changes in two genes which have never before been implicated in an iron-dopamine pathway, Cxcl12 and hemoglobin, beta adult chain PMID: 23911809
  98. Interfering with the CXCL12-scavenging activity of CXCR7 causes loss of CXCR4 function as a consequence of excessive CXCL12-mediated CXCR4 activation and degradation. PMID: 24718993
  99. identified a novel CD45 phosphorylation event in hematopoietic progenitor cells that correlated with motile response and governed by the chemotactic factor CXCL12 PMID: 23997015
  100. IL-17 drives the differentiation of lung stroma toward podoplanin-positive CXCL12-expressing cells that allow follicle formation even in the absence of follicular dendritic cells. PMID: 24663215

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity Highest expression levels detected in kidney, liver, spleen and muscle. Isoform Alpha is expressed ubiquitously but at varying levels, while isoform Beta displays tissue-specific expression, with expression detected in kidney, liver, heart, spleen and mus
Database Links

KEGG: mmu:20315

STRING: 10090.ENSMUSP00000072800

UniGene: Mm.303231

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