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Rat α-Smooth muscle actin,α-SMA ELISA Kit

Citations (10) Protocol MSDS
Code CSB-E14027r
Size 96T,5×96T,10×96T
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Product Details

Target Name
actin, alpha 2, smooth muscle, aorta
Alternative Names
Acta2 ELISA kit; Actsa ELISA kit; ActvsActin ELISA kit; aortic smooth muscle ELISA kit; Alpha-actin-2) [Cleaved into: Actin ELISA kit; aortic smooth muscle ELISA kit; intermediate form] ELISA kit
Abbreviation
ACTA2
Uniprot No.
P62738
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-101  
1:2 Average % 90  
Range % 85-94  
1:4 Average % 87  
Range % 82-91  
1:8 Average % 102  
Range % 97-109  
Recovery
The recovery of rat α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 93 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.595 2.451 2.523 2.431  
25 1.689 1.601 1.645 1.553  
12.5 0.972 0.893 0.933 0.841  
6.25 0.493 0.512 0.503 0.411  
3.13 0.330 0.306 0.318 0.226  
1.56 0.206 0.210 0.208 0.116  
0.78 0.126 0.121 0.124 0.032  
0 0.094 0.089 0.092 　  
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The rat α-SMA ELISA Kit is engineered for accurate measurement of rat α-SMA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the α-SMA content in the sample. The color intensity is positively correlated with α-SMA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

α-SMA, also called ACTA2, is the actin isoform that predominates within vascular smooth muscle cells. The expression of α-SMA defines the phenotypic transition to myofibroblasts and is a major contributor to increased contractile force generation by these cells. α-SMA contributes to cell-generated mechanical tension and is normally restricted to cells of vascular smooth muscle. It is used as a marker for a subset of activated fibrogenic cells, myofibroblasts, which are important effector cells of tissue fibrogenesis. α-SMA is important dor fibrogenesis. Additionally, α-SMA can enhance the contractile activity of myofibroblasts.

Citations

Related Products

Acta2 Proteins

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Target Background

Function
(From Uniprot)
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Gene References into Functions
  1. considering the steroid hormone imbalance in late life period, as well as, the role of this imbalance in prostatic lesion development and progression, the aim of the study herein was to characterize and correlate prolactin, EGFR, alpha-actin and vimentin immunoreactivity in the prostatic epithelium and stroma of elderly rats, following steroid hormone imbalance. PMID: 27021728
  2. Data suggest that the expression of actin alpha 2 smooth muscle (alpha-SMA) plays certain roles in the neuroma-associated pain, either as a direct cause of pain or as an indirect marker of existence of local mechanical stimuli. PMID: 27021914
  3. Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis. PMID: 25681120
  4. Smooth muscle alpha-actin is present in the cervix of virgin, 11 day, and 20 day pregnant rats. PMID: 25108144
  5. Data suggest that the myosin-II mediated drebrin A-actin exodus might be an initial event in chemical long-term potentiation (LTP) induction, triggering actin polymerization and spine enlargement. PMID: 24465547
  6. Acta2 is important for myofibroblast cells motility, contraction and regulation of signaling processes during wound healing. PMID: 24204762
  7. Data from several animal models suggest that up-regulation of expression of Tgfb1 (transforming growth factor beta 1) and Acta2 in proximal tubular cells is key to development of renal tubular fibrosis in chronic kidney disease/renal hypertension. PMID: 24601883
  8. Smooth muscle actin expression increased during lung injury induced by hyperoxia, and could play an important role in pulmonary fibrosis remodeling. PMID: 23040780
  9. Smooth muscle alpha-actin was increased on day 9 of bleomycin induced pulmonary fibrosis in rats, and is involved in the mechanism of N-acetylcysteine alleviation of idiopathic pulmonary fibrosis. PMID: 23006535
  10. novel populations of alphaSMA+ precursors of pericytes are born on the subventricular zones then migrate into the peri-infarct region PMID: 22805872
  11. Tendon cells are capable of actively contracting lax tendons through an alpha smooth muscle actin-based mechanism. PMID: 22517354
  12. Hepatocyte growth factor can relieve chronic renal failure, and this protection is associated with the down-regulation of alpha-SMA expression in mesangial cells and renal interstitium. PMID: 22680062
  13. Leptin potentiates acetaldehyde-induced hepatic stellate cell activation and transdifferentiation as evidenced by increased alpha-smooth muscle actin expression. PMID: 21294755
  14. The results suggest that alpha-SMA containing stress fibers contribute to myofibroblast arrhythmogeneicity. After ablation of this cytoskeletal component, cells lose their arrhythmic effects on cardiomyocytes. PMID: 21921266
  15. alpha-SMA expression in renal interstitial fibroblasts, i.e., myofibroblast transdifferentiation, accelerates the contraction of the tubulointerstitial fibrosis tissue via the Ca(2)+-dependent pathway PMID: 21630215
  16. Reactivation of fetal structural genes of vascular smooth muscle alpha-actin and its ectopic expression is associated with the cardiac allograft vasculopathy and fibrosis. PMID: 19411749
  17. The expression of alpha -SMA is increased dramatically during the fibrosis, and is positively associated with the expression of alpha -AR, beta(1)-AR and beta(2)-AR. PMID: 19785950
  18. Transcriptional regulation of a contractile gene by mechanical forces applied through integrins in osteoblasts PMID: 11953441
  19. smooth muscle alpha-actin expression requires a transforming growth factor-beta control element PMID: 12970361
  20. Tacrolimua and sirolimus both increased kidney transplantation TGF-beta1 and alpha-SMA levels. PMID: 15316360
  21. There was a significant increase in smooth muscle alpha-actin located around regenerating hepatic nodules. PMID: 15814362
  22. results provide the first in vivo evidence for the importance of the intronic CArG and transforming growth factor-beta1 control element cis-elements in the regulation of alpha-SMA expression in myofibroblasts in granulation tissue PMID: 15855636
  23. Myometrial expression of alpha-SM-actin mRNA and protein was high throughout pregnancy. PMID: 15972885
  24. Results identify alpha-smooth muscle actin as a mechanosensitive protein that is recruited to stress fibers under high tension. PMID: 16401722
  25. Force-mediated actin filament assembly promotes nuclear translocation of MRTF and subsequent activation of the SMA promoter to enhance SMA expression. PMID: 17456553
  26. Olfactory ensheathing cells synthesize smooth muscle alpha-actin (and a variety of actin-binding proteins including caldesmon) providing compelling evidence that these cells are different from Schwann cells. PMID: 17492622
  27. These findings indicate the existence of alpha-smooth muscle actin-positive endothelial cells in adult aortic endothelium and the possible association with progression of atherosclerosis. PMID: 19285011

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Actin family
Database Links

KEGG: rno:81633

UniGene: Rn.195319

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