Rat α-Smooth muscle actin,α-SMA ELISA Kit

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Description

The rat α-SMA ELISA Kit is engineered for accurate measurement of rat α-SMA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the α-SMA content in the sample. The color intensity is positively correlated with α-SMA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

α-SMA, also called ACTA2, is the actin isoform that predominates within vascular smooth muscle cells. The expression of α-SMA defines the phenotypic transition to myofibroblasts and is a major contributor to increased contractile force generation by these cells. α-SMA contributes to cell-generated mechanical tension and is normally restricted to cells of vascular smooth muscle. It is used as a marker for a subset of activated fibrogenic cells, myofibroblasts, which are important effector cells of tissue fibrogenesis. α-SMA is important dor fibrogenesis. Additionally, α-SMA can enhance the contractile activity of myofibroblasts.

Target Name

actin, alpha 2, smooth muscle, aorta

Alternative Names

Acta2 ELISA kit; Actsa ELISA kit; ActvsActin ELISA kit; aortic smooth muscle ELISA kit; Alpha-actin-2) [Cleaved into: Actin ELISA kit; aortic smooth muscle ELISA kit; intermediate form] ELISA kit

Abbreviation

ACTA2

Uniprot No.

P62738

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	90
	Range %	85-94
1:4	Average %	87
	Range %	82-91
1:8	Average %	102
	Range %	97-109

Recovery

The recovery of rat α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.595	2.451	2.523	2.431
25	1.689	1.601	1.645	1.553
12.5	0.972	0.893	0.933	0.841
6.25	0.493	0.512	0.503	0.411
3.13	0.330	0.306	0.318	0.226
1.56	0.206	0.210	0.208	0.116
0.78	0.126	0.121	0.124	0.032
0	0.094	0.089	0.092

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Gene References into Functions	considering the steroid hormone imbalance in late life period, as well as, the role of this imbalance in prostatic lesion development and progression, the aim of the study herein was to characterize and correlate prolactin, EGFR, alpha-actin and vimentin immunoreactivity in the prostatic epithelium and stroma of elderly rats, following steroid hormone imbalance. PMID: 27021728 Data suggest that the expression of actin alpha 2 smooth muscle (alpha-SMA) plays certain roles in the neuroma-associated pain, either as a direct cause of pain or as an indirect marker of existence of local mechanical stimuli. PMID: 27021914 Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis. PMID: 25681120 Smooth muscle alpha-actin is present in the cervix of virgin, 11 day, and 20 day pregnant rats. PMID: 25108144 Data suggest that the myosin-II mediated drebrin A-actin exodus might be an initial event in chemical long-term potentiation (LTP) induction, triggering actin polymerization and spine enlargement. PMID: 24465547 Acta2 is important for myofibroblast cells motility, contraction and regulation of signaling processes during wound healing. PMID: 24204762 Data from several animal models suggest that up-regulation of expression of Tgfb1 (transforming growth factor beta 1) and Acta2 in proximal tubular cells is key to development of renal tubular fibrosis in chronic kidney disease/renal hypertension. PMID: 24601883 Smooth muscle actin expression increased during lung injury induced by hyperoxia, and could play an important role in pulmonary fibrosis remodeling. PMID: 23040780 Smooth muscle alpha-actin was increased on day 9 of bleomycin induced pulmonary fibrosis in rats, and is involved in the mechanism of N-acetylcysteine alleviation of idiopathic pulmonary fibrosis. PMID: 23006535 novel populations of alphaSMA+ precursors of pericytes are born on the subventricular zones then migrate into the peri-infarct region PMID: 22805872 Tendon cells are capable of actively contracting lax tendons through an alpha smooth muscle actin-based mechanism. PMID: 22517354 Hepatocyte growth factor can relieve chronic renal failure, and this protection is associated with the down-regulation of alpha-SMA expression in mesangial cells and renal interstitium. PMID: 22680062 Leptin potentiates acetaldehyde-induced hepatic stellate cell activation and transdifferentiation as evidenced by increased alpha-smooth muscle actin expression. PMID: 21294755 The results suggest that alpha-SMA containing stress fibers contribute to myofibroblast arrhythmogeneicity. After ablation of this cytoskeletal component, cells lose their arrhythmic effects on cardiomyocytes. PMID: 21921266 alpha-SMA expression in renal interstitial fibroblasts, i.e., myofibroblast transdifferentiation, accelerates the contraction of the tubulointerstitial fibrosis tissue via the Ca(2)+-dependent pathway PMID: 21630215 Reactivation of fetal structural genes of vascular smooth muscle alpha-actin and its ectopic expression is associated with the cardiac allograft vasculopathy and fibrosis. PMID: 19411749 The expression of alpha -SMA is increased dramatically during the fibrosis, and is positively associated with the expression of alpha -AR, beta(1)-AR and beta(2)-AR. PMID: 19785950 Transcriptional regulation of a contractile gene by mechanical forces applied through integrins in osteoblasts PMID: 11953441 smooth muscle alpha-actin expression requires a transforming growth factor-beta control element PMID: 12970361 Tacrolimua and sirolimus both increased kidney transplantation TGF-beta1 and alpha-SMA levels. PMID: 15316360 There was a significant increase in smooth muscle alpha-actin located around regenerating hepatic nodules. PMID: 15814362 results provide the first in vivo evidence for the importance of the intronic CArG and transforming growth factor-beta1 control element cis-elements in the regulation of alpha-SMA expression in myofibroblasts in granulation tissue PMID: 15855636 Myometrial expression of alpha-SM-actin mRNA and protein was high throughout pregnancy. PMID: 15972885 Results identify alpha-smooth muscle actin as a mechanosensitive protein that is recruited to stress fibers under high tension. PMID: 16401722 Force-mediated actin filament assembly promotes nuclear translocation of MRTF and subsequent activation of the SMA promoter to enhance SMA expression. PMID: 17456553 Olfactory ensheathing cells synthesize smooth muscle alpha-actin (and a variety of actin-binding proteins including caldesmon) providing compelling evidence that these cells are different from Schwann cells. PMID: 17492622 These findings indicate the existence of alpha-smooth muscle actin-positive endothelial cells in adult aortic endothelium and the possible association with progression of atherosclerosis. PMID: 19285011 Show More Hide All
Subcellular Location	Cytoplasm, cytoskeleton.
Protein Families	Actin family
Database Links	KEGG: rno:81633 UniGene: Rn.195319

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Check Code：

Code	CSB-E14027r
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.