Rat α-Smooth muscle actin,α-SMA ELISA Kit

Code CSB-E14027r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P62738
Lead Time 3-5 working days
Abbreviation ACTA2
Target Name actin, alpha 2, smooth muscle, aorta
Alias AAT6, ACTSA, alpha 2 actin|alpha-cardiac actin|growth-inhibiting gene 46
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-101  
1:2 Average % 90  
Range % 85-94  
1:4 Average % 87  
Range % 82-91  
1:8 Average % 102  
Range % 97-109  
Recovery
The recovery of rat α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 93 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.595 2.451 2.523 2.431  
25 1.689 1.601 1.645 1.553  
12.5 0.972 0.893 0.933 0.841  
6.25 0.493 0.512 0.503 0.411  
3.13 0.330 0.306 0.318 0.226  
1.56 0.206 0.210 0.208 0.116  
0.78 0.126 0.121 0.124 0.032  
0 0.094 0.089 0.092    
References
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Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Location Cytoplasm, cytoskeleton
Protein Families Actin family
Database Links

KEGG: rno:81633

UniGene: Rn.195319

Pathway Vascular smooth muscle contraction
Apelin signaling pathway

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