Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

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Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Specification
Target

Code

CSB-E08594r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P23693

Lead Time

3-5 working days

Abbreviation

cTn-Ⅰ

Target Name

Cardiac Troponin Ⅰ(cTn-Ⅰ)

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	98
	Range %	91-104
1:4	Average %	99
	Range %	94-105
1:8	Average %	87
	Range %	82-93

Recovery

The recovery of rat cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-92
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.321	2.220	2.271	2.118
1000	1.340	1.441	1.391	1.238
500	0.814	0.835	0.825	0.672
250	0.529	0.540	0.535	0.382
125	0.345	0.334	0.340	0.187
62.5	0.261	0.250	0.256	0.103
31.25	0.197	0.193	0.195	0.042
0	0.153	0.152	0.153

References

Dihydromyricetin improves glucose and lipid metabolism and exerts anti-inflammatory effects in nonalcoholic fatty liver disease: A randomized controlled trial Chen S. et al,Pharmacol Res.,2015 Sep;99:74-81.
Regulation of signal transducer and activator of transcription 3 and apoptotic pathways by betaine attenuates isoproterenol-induced acute myocardial injury in rats. Zheng P.et al,Hum Exp Toxicol.,34(5):538-47
Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015 Sep;20(9):1164-75
Propofol Through Upregulating Caveolin-3 Attenuates Post-Hypoxic Mitochondrial Damage and Cell Death in H9C2 Cardiomyocytes During Hyperglycemia Deng F.et al,Cell Physiol Biochem,Nov 9
Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis Wen-Juan Fu.et al,J Ethnopharmacol. ,Jan 4;195:118-126
Involvement of brain natriuretic peptide signaling pathway in the cardioprotective action of sitagliptin Samah S.Abbas.et al,Pharmacological Reports,Feb 7
Study on Cardiotoxicity and Mechanism of “Fuzi” Extracts Based on Metabonomics Huang G, et al,International Journal of Molecular Sciences,November 7
Protective effects of resveratrol on biomarkers of oxidative stress, biochemical and histopathological changes induced by sub-chronic oral glyphosate-based herbicide in rats Ruhi Turkmen, et al,Toxicology Research,January 7

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Function	Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Families	Troponin I family
Database Links	KEGG: rno:29248 STRING: 10116.ENSRNOP00000024640 UniGene: Rn.64141
Pathway	cAMP signaling pathway Cardiac muscle contraction

Tnni3 Protein

Tnni3 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP340566RA CSB-EP340566RA CSB-BP340566RA CSB-MP340566RA	Recombinant Rat Troponin I, cardiac muscle(Tnni3)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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