| Code |
CSB-E08594r |
| See More Details |
 |
| Product Type |
ELISA Kit |
| Size |
96T,5×96T,10×96T |
| Uniprot No. |
P23693
|
| Lead Time |
3-5 working days |
| Abbreviation |
cTn-Ⅰ |
| Target Name |
Cardiac Troponin Ⅰ(cTn-Ⅰ) |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
92 |
|
| Range % |
88-98 |
|
| 1:2 |
Average % |
98 |
|
| Range % |
91-104 |
|
| 1:4 |
Average % |
99 |
|
| Range % |
94-105 |
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| 1:8 |
Average % |
87 |
|
| Range % |
82-93 |
|
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| Recovery |
| The recovery of rat cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
88 |
82-92 |
|
| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 2000 |
2.321 |
2.220 |
2.271 |
2.118 |
|
| 1000 |
1.340 |
1.441 |
1.391 |
1.238 |
|
| 500 |
0.814 |
0.835 |
0.825 |
0.672 |
|
| 250 |
0.529 |
0.540 |
0.535 |
0.382 |
|
| 125 |
0.345 |
0.334 |
0.340 |
0.187 |
|
| 62.5 |
0.261 |
0.250 |
0.256 |
0.103 |
|
| 31.25 |
0.197 |
0.193 |
0.195 |
0.042 |
|
| 0 |
0.153 |
0.152 |
0.153 |
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| References |
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Preparation and appraisal of self assembled valsartan-loaded amalgamated Pluronic F127/Tween 80 polymeric micelles: Boosted cardioprotection via regulation of Mhrt/Nrf2 and Trx1 pathways in cisplatin-induced cardiotoxicity Aboud HM, et al,Journal of Drug Targeting,2019
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Protection of Myocardial Ischemia-Reperfusion by Therapeutic Hypercapnia: a Mechanism Involving Improvements in Mitochondrial Biogenesis and Function Chi L, et al,Journal of Cardiovascular Translational Research,2019
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The protective effect of kaempferol on heart via the regulation of Nrf2, NF-κβ, and PI3K/Akt/GSK-3β signaling pathways in isoproterenol-induced heart failure in diabetic rats Zhang L, et al,Drug development research,2019
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Antioxidant and cytoprotective effects of N-acetylcysteine against subchronic oral glyphosate-based herbicide-induced oxidative stress in rats Turkmen R, et al,Environmental science,2019
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Dexmedetomidine Preconditioning Protects Cardiomyocytes Against Hypoxia/Reoxygenation-Induced Necroptosis by Inhibiting HMGB1-Mediated Inflammation Chen J, et al,Cardiovascular Drugs And Therapy,2019
-
Protective effects of resveratrol on biomarkers of oxidative stress, biochemical and histopathological changes induced by sub-chronic oral glyphosate-based herbicide in rats Ruhi Turkmen, et al,Toxicology Research,2019
-
Study on Cardiotoxicity and Mechanism of “Fuzi” Extracts Based on Metabonomics Huang G, et al,International Journal of Molecular Sciences,2018
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Involvement of brain natriuretic peptide signaling pathway in the cardioprotective action of sitagliptin Samah S.Abbas.et al,Pharmacological Reports,2018
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Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis Wen-Juan Fu.et al,J Ethnopharmacol. ,2017
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Propofol Through Upregulating Caveolin-3 Attenuates Post-Hypoxic Mitochondrial Damage and Cell Death in H9C2 Cardiomyocytes During Hyperglycemia Deng F.et al,Cell Physiol Biochem,2017
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Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015
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Regulation of signal transducer and activator of transcription 3 and apoptotic pathways by betaine attenuates isoproterenol-induced acute myocardial injury in rats. Zheng P.et al,Hum Exp Toxicol.,2015
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Dihydromyricetin improves glucose and lipid metabolism and exerts anti-inflammatory effects in nonalcoholic fatty liver disease: A randomized controlled trial Chen S. et al,Pharmacol Res.,2015
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