Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Code CSB-E08594r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P23693
Lead Time 3-5 working days
Abbreviation cTn-Ⅰ
Target Name Cardiac Troponin Ⅰ(cTn-Ⅰ)
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-98  
1:2 Average % 98  
Range % 91-104  
1:4 Average % 99  
Range % 94-105  
1:8 Average % 87  
Range % 82-93  
Recovery
The recovery of rat cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 82-92  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.321 2.220 2.271 2.118  
1000 1.340 1.441 1.391 1.238  
500 0.814 0.835 0.825 0.672  
250 0.529 0.540 0.535 0.382  
125 0.345 0.334 0.340 0.187  
62.5 0.261 0.250 0.256 0.103  
31.25 0.197 0.193 0.195 0.042  
0 0.153 0.152 0.153    
References
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Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Families Troponin I family
Database Links

KEGG: rno:29248

STRING: 10116.ENSRNOP00000024640

UniGene: Rn.64141

Pathway cAMP signaling pathway
Cardiac muscle contraction

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