Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Instructions
Code CSB-E08594r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Cardiac Troponin Ⅰ(cTn-Ⅰ)
Alternative Names Tnni3 ELISA Kit; Ctni ELISA Kit; Tni ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
Abbreviation cTn-I
Uniprot No. P23693
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-98  
1:2 Average % 98  
Range % 91-104  
1:4 Average % 99  
Range % 94-105  
1:8 Average % 87  
Range % 82-93  
Recovery
The recovery of rat cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 82-92  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.321 2.220 2.271 2.118  
1000 1.340 1.441 1.391 1.238  
500 0.814 0.835 0.825 0.672  
250 0.529 0.540 0.535 0.382  
125 0.345 0.334 0.340 0.187  
62.5 0.261 0.250 0.256 0.103  
31.25 0.197 0.193 0.195 0.042  
0 0.153 0.152 0.153    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. Pim-1 is a novel kinase that phosphorylates cTnI primarily at Ser23/24 and Ser150 in cardiomyocytes, which in turn may modulate myofilament function under a variety of physiological and pathophysiological conditions. PMID: 29544221
  2. Cardiac troponin I mutation P83S present in hypertrophic cardiomyopathy changes the contractile myofibril properties and modulation by PKA-mediated phosphorylation. PMID: 27150586
  3. Troponin I interaction with calcium and Troponin C in cardiac muscle PMID: 28864299
  4. Compared to cTnI(WT), both truncations displayed greater Ca(2+)-sensitivity and faster cross-bridge attachment rates at both SLs. Furthermore, cTnI(1-167) slowed MgADP release rate and enhanced cross-bridge binding. Our findings imply that cTnI-MD truncations affect the blocked-to closed-state transition(s) and destabilize the closed-state position of tropomyosin. PMID: 28958680
  5. PKC phosphorylates cardiac troponin I (cTnI) S23/24, S43/45 and T144 to fine tune myocyte function. PMID: 28587770
  6. These findings demonstrate that S-nitrosylation and S-glutathionylation exert opposing effects on Ca(2+) sensitivity in mammalian FT muscle fibers, mediated by competitive actions on Cys134 of TnIf. PMID: 27974300
  7. the interaction between cTnC and cTnI in skinned papillary muscle strips is dependent on sarcomere length PMID: 26944554
  8. cTnIS43/45N is a functionally conservative substitution, and may be appropriate for use as a phospho-null in rodent models designed for studies on PKC modulation of cardiac performance PMID: 26869200
  9. This study suggests that cTnI point of care tests can accurately determine heat stroke (HS) severity and could serve as simple, portable, cost-effective HS field tests. PMID: 26290107
  10. The present study utilizes viral gene transfer of cTnI with phosphomimetic S43D and/or S45D substitutions to evaluate their individual and combined influences on function in intact adult cardiac myocytes. PMID: 25481661
  11. Results suggest that weakened troponin C interaction with cTnI, via PKA phosphorylation of cTnI, may slow thin filament activation and result in increased myofilament relaxation kinetics PMID: 25185555
  12. The findings elucidate the pathogenesis of MI, and the gradual increase in serum adropin could be a novel diagnostic marker and serve as an alternative to troponin-I measurement for diagnosing MI. PMID: 24932661
  13. molecular determinants of cardiac myocyte performance as conferred by isoform-specific TnI residues PMID: 24853739
  14. Arsenic induced ventricular hypertrophy occurs via MEF2A/CAMKK2/CALM3/TNNI3 signaling. PMID: 25089838
  15. Data indicate that the serum TnI level was significantly greater in the acute coronary syndrome (ACS) group compared to the control group. PMID: 23904327
  16. A cutoff value of 4.8ng/mL for cTnI could be used as early as 8h after MI to accurately identify infarct in this model, whereas echocardiographic images taken 48h after MI predicted the infarcted area 14days after MI. PMID: 23764111
  17. Mutation in the C helix of cTnC can reduce Ca2+ binding affinity and cTnC-cTnI interaction. PMID: 23454346
  18. Hypertrophic cardiomyopathy related mutations R146G/Q and R163W impact interactions between cTnI and cardiac troponin C or actin. PMID: 23246786
  19. Aan increase in calpain activity may enhance cTnI degradation in the myocardium of tail-suspended rats. PMID: 20945043
  20. Normal developing myocardium and skeletal muscle transiently share both sk-fMHC and cTn-I proteins. PMID: 22808244
  21. the functional impact of cardiac troponin I (cTnI) phosphorylation by protein kinase A PMID: 22684024
  22. cTnC, cTnI, cTnT and cTm are not only present in myofilaments of ventricular cardiomyocytes in culture but are also within their nuclei; significantly, these four proteins appear between days 3 and 5 in both myofilaments and nuclei PMID: 22364878
  23. differential histidine ionization may be necessary for cTnI A164H to act as a molecular sensor capable of modulating sarcomere performance in response to changes in the cytosolic milieu PMID: 22500757
  24. Structural dynamics of C-domain of cardiac troponin I protein in reconstituted thin filament. PMID: 22207765
  25. PKC phosphorylation of cTnI may be maladaptive and potentially associated with cardiac dysfunction PMID: 22052912
  26. Cardiac function and phosphorylation of PLB and cTnI were compared in pacing, isoproterenol treatment, and combined pacing and isoproterenol treatment in isolated working heart. PMID: 21876643
  27. Ulinastatin may protect myocardium from the damage resulted from sepsis in a rat model, probably by lowering expressions of cTnI, TNF-alpha and ET-1. PMID: 20594472
  28. Data show that dual expression of two CM mutants, tropomyosin mutant A63V and cardiac troponin mutant R146G, were shown to additively slow myocyte relaxation beyond either mutant studied in isolation. PMID: 20161772
  29. Time-dependent cTnI breakdown occurs during global ischemia independent of reperfusion. cTnI breakdown during ischemia is further increased in presence of antioxidants. ROS generated during ischemia may play cTnI protective role. PMID: 15142843
  30. cardiac Troponin I Thr144 plays an important role in the acute acceleration of relaxation, whereas Ser23/Ser24 contributes to relaxation during more prolonged activation of protein kinase C by endothelin PMID: 16236710
  31. GATA elements control repression of cTNI promoter activity in skeletal muscle cells. PMID: 17875210
  32. Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. PMID: 18397962
  33. cTnI-R145G expression influences the response to adrenergic stimulation dependent on the receptor subtype PMID: 18548271
  34. Metabolic inhibition of cardiomyocytes induces a parallel release of intact cTnI and its degradation products, starting only after onset of irreversible cardiomyocyte damage. PMID: 18721805
  35. the nNOS-PMCA4b complex regulates contractility via cAMP and phosphorylation of both PLB and cTnI. PMID: 19278978

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Protein Families Troponin I family
Database Links

KEGG: rno:29248

STRING: 10116.ENSRNOP00000024640

UniGene: Rn.64141

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