Rat Glutathione Peroxidase (GSH-PX) ELISA kit

Instructions
Code CSB-E12146r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name glutathione peroxidase 1
Alternative Names Gpx1Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit
Abbreviation GPX1
Uniprot No. P04041
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 mIU/mL-800 mIU/mL
Sensitivity 3.12 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:200Average %92
Range %85-100
1:400Average %97
Range %94-101
1:800Average %95
Range %90-99
1:1600Average %93
Range %87-98
Recovery
The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9486-100
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
8002.251 2.299 2.275 2.119
4001.845 1.834 1.840 1.684
2001.301 1.324 1.313 1.157
1000.892 0.876 0.884 0.728
500.553 0.590 0.572 0.416
250.395 0.381 0.388 0.232
12.50.251 0.234 0.243 0.087
00.155 0.157 0.156  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Protects the hemoglobin in erythrocytes from oxidative breakdown.
Gene References into Functions
  1. Knockdown of nNOS or GPx1 was specific and had broad effects on downstream genes, though it only modestly ameliorated traumatic brain injury-induced working memory deficits, despite widespread genomic changes. PMID: 29016640
  2. The glutathione peroxidase activity and p110-PI3K/Thr308-Akt are both modulated in the same way by oxygen. PMID: 24401635
  3. VPO1 plays an important role in senescence of endothelial cells and endothelial dysfunction by induction of oxidative stress and inflammatory reaction in type 2 diabetic rats. PMID: 26142204
  4. glutathione peroxidase 1 immunoreactivity was diffusely cytoplasmic in some of the hepatocytes PMID: 24867493
  5. Dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress. PMID: 24962481
  6. It represents the pivotal antioxidant enzyme in vascular endothelium and exerts a protective effect against the presence of coronary artery disease PMID: 24389817
  7. data collectively indicate that GDNF is capable of inducing anti-oxidant activities of intra-cellular GPX-1 and that growth-promoting potential of GDNF and anti-oxidant properties of GPX-1 can, in concert, maximize survival of dopaminergic neurons. PMID: 23472740
  8. Upon selenium fluctuation, the modulation of UGA recoding efficiency depends on the nature of the SECIS, with Gpx1 being more sensitive than Gpx4. PMID: 22614831
  9. GPx levels in the cortex and hippocampus were increased in OLETF rats compared with LETO rats, with an inverse correlation between GPx in the hippocampus and learning score. PMID: 22981416
  10. The glutathione peroxidase represents an important part of the antioxidant defense system. PMID: 20524817
  11. expression of glutathione peroxidase 1 and glutathione reductase via GSH did not appear to coordinate with development, tissue localization or under oxidative stress PMID: 12516874
  12. excess amounts of PLOOH are efficiently eliminated through the hydrolysis by PLA2, and the subsequent reduction of FA hydroperoxide by GPx is the critical step for complete detoxification of oxidized phospholipids in the stomach PMID: 12934674
  13. Over-expression gpx1 genes in primary hippocampal or cortical cultures resulted in increased enzymatic activity of the cognate protein. PMID: 14713308
  14. Gxp1 levels was increased indicating that a reduction in oxidative damage protection may be responsible for accelerated telomere shortening over time, resulting in increased cellular senescence, loss of renal function, and death in male rats. PMID: 16189290
  15. Novel sites of Gpx1 expression were observed in colon lymphatic tissue. PMID: 16229841
  16. Our results suggest that impairment of the GPx (including glutathione)-mediated antioxidant system contributed to epileptogenesis in GEPR-9s. PMID: 18226427
  17. docosahexaenoic acid sensitizes breast cancer cells to anthracyclines through loss of glutathione peroxidase (GPx1) response PMID: 18267129
  18. Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis. PMID: 19234057
  19. red blood cell glutathione peroxidase (Gpx1) activity in old female rats. PMID: 19398061
  20. Whole blood selenoprotein mRNA levels can be used as molecular biomarkers for assessing Selenium requirements. PMID: 19855070

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Subcellular Location Cytoplasm
Protein Families Glutathione peroxidase family
Tissue Specificity Expressed in liver and lung.
Database Links

KEGG: rno:24404

STRING: 10116.ENSRNOP00000066577

UniGene: Rn.11323

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