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| Code | CSB-E12146r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 94-101 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 86-100 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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CUSABIO's rat GPX1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of GPX1 concentrations in serum, plasma, and tissue homogenates. This assay exclusively recognizes rat GPX1. The quantitative sandwich ELISA technique of this kit is based on GPX1 antibody-GPX1 antigen interactions and an HRP colorimetric detection system to detect the levels of GPX1 in samples. The intensity of the color is positively proportional to the amount of bound GPX1 in the initial step.
GPX1 is a selenocysteine-containing intracellular antioxidant enzyme that enzymatically reduces hydrogen peroxide to water to limit its harmful effects. In mitochondria, GPX1 is thought to protect mitochondrial DNA from oxidative damage. GPX1 overexpression is linked to increased protection against oxidative stress in cell culture models as well ad in genetic mouse models. GPX1 is also involved in the regulation of the cell cycle by controlling cellular levels of hydrogen peroxide. Okubo et al. showed that GPX1 supports cancer cell survival and increases the incidence of metastasis.
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