Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit

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Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Name: Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit
Brand: CUSABIO
SKU: CSB-E05129r

^Instructions

Code	CSB-E05129r
Size	96T,5×96T,10×96T

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Target Data

Target Name

bone gamma-carboxyglutamate (gla) protein

Alternative Names

RP11-54H19.5, BGP, OC, PMF1, bone Gla protein|bone gamma-carboxyglutamate (gla) protein (osteocalcin)|gamma-carboxyglutamic acid-containing protein|osteocalcin

Abbreviation

BGLAP

Uniprot No.

P04640

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, urine

Detection Range

0.312 pg/mL-20 pg/mL

Sensitivity

0.078 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	100
	Range %	95-106
1:400	Average %	89
	Range %	84-95
1:800	Average %	88
	Range %	84-92
1:1600	Average %	90
	Range %	85-95

Recovery

The recovery of rat OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-90
EDTA plasma (n=4)	104	99-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
20	2.306	2.368	2.337	2.152
10	1.954	1.876	1.915	1.730
5	1.583	1.469	1.526	1.341
2.5	1.071	1.096	1.084	0.899
1.25	0.705	0.748	0.727	0.542
0.625	0.528	0.567	0.548	0.363
0.312	0.317	0.302	0.310	0.125
0	0.186	0.183	0.185

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

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Possible osteoprotective effects of myricetin in STZ induced diabetic osteoporosis in rats Xiaozhou Ying, et al,European Journal of Pharmacology,2019
Toll-like receptor 4 knockout protects against diabetic-induced imbalance of bone metabolism via autophagic suppression Yongze Zhang, et al,Molecular Immunology,2019
Exosomal hsa-miR-21-5p derived from growth hormone-secreting pituitary adenoma promotes abnormal bone formation in acromegaly Yuanyuan Xiong, et al,Translational Research,2019
Evaluation of the osteoprotective potential of whey derived-antioxidative (YVEEL) and angiotensin-converting enzyme inhibitory (YLLF) bioactive peptides in ovariectomised rats Masum Pandey.et al,FOOD & FUNCTION,2018
Soybean-Hop Alleviates Estrogen Deficiency-Related Bone Loss and Metabolic Dysfunction in Ovariectomized Rats Fed a High-Fat Diet Dasom Noh.et al,Molecules,2018
Animal model having hypoparathyroidism and method for producing the same Han Su.et al,/,2018
Advanced oxidation protein products accelerated the bone loss and weakened bone strength in ovariectomised rats Shuai Zheng.et al,Int J Clin Exp Pathol ,2017
Tetrahydroxystilbene glucoside isolated from Polygonum multiflorum Thunb. demonstrates osteoblast differentiation promoting activity Yayuan Zheng.et al,Experimental and Therapeutic Medicine,2017
Effects of Aqueous Extract of Phyllostachyos Caulis in Taeniam on Longitudinal Bone Growth in Adolescent Rats Chung YH. et al,Planta Med.,2016
Effects of Fructus Chaenomelis on longitudinal bone growth in adolescent rats hun Lee C.et al,International Journal of Phytomedicine,2015
Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone /,Acta Pharmacologica Sinica,2012
Effects of pro-inflammatory cytokines on mineralization potential of rat dental pulp stem cells Xuechao Yang et al,Journal of Tissue Engineering and Regenerative Medicine,2011
A role for suppressed bone formation favoring catch-up fat in the pathophysiology of catch-up growth after food restriction Xiangfei Guo et al,European Journal of Nutrition,2011

■ Q&A

I would like to know what wavelength this product needs to be read at to make sure we have the appropriate filters. Also, we have some mouse cells lines, will this product work for those cell lines as well?

Thanks for your inquiry.
We use TMB substrate. The detection wavelength must be 450nm. The absorbance value will be very good at this wavelength. Considering there may be scratches at the bottom of the vial when you add samples, we suggest you use dual wavelength( detection wavelength and correction wavelength).
We don't suggest you use this kit to test mouse cells lines. If you want to test it, you can do a pretest first.

■ Customer Reviews

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Function	Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Subcellular Location	Secreted
Protein Families	Osteocalcin/matrix Gla protein family
Database Links	KEGG: rno:25295 STRING: 10116.ENSRNOP00000026530 UniGene: Rn.9722

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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