Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Instructions
Code CSB-E05129r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name bone gamma-carboxyglutamate (gla) protein
Alternative Names Bglap ELISA Kit; Bglap2 ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
Abbreviation BGLAP
Uniprot No. P04640
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 0.312 pg/mL-20 pg/mL
Sensitivity 0.078 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 100  
Range % 95-106  
1:400 Average % 89  
Range % 84-95  
1:800 Average % 88  
Range % 84-92  
1:1600 Average % 90  
Range % 85-95  
Recovery
The recovery of rat OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 83-90  
EDTA plasma (n=4) 104 99-109  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
20 2.306 2.368 2.337 2.152  
10 1.954 1.876 1.915 1.730  
5 1.583 1.469 1.526 1.341  
2.5 1.071 1.096 1.084 0.899  
1.25 0.705 0.748 0.727 0.542  
0.625 0.528 0.567 0.548 0.363  
0.312 0.317 0.302 0.310 0.125  
0 0.186 0.183 0.185    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

I would like to know what wavelength this product needs to be read at to make sure we have the appropriate filters. Also, we have some mouse cells lines, will this product work for those cell lines as well?

A:
Thanks for your inquiry.
We use TMB substrate. The detection wavelength must be 450nm. The absorbance value will be very good at this wavelength. Considering there may be scratches at the bottom of the vial when you add samples, we suggest you use dual wavelength( detection wavelength and correction wavelength).
We don't suggest you use this kit to test mouse cells lines. If you want to test it, you can do a pretest first.
 Customer Reviews
  • Sample type: Serum
    Sample species: Rat
    Review: 1.Ctrl; 2.Sham(no ovariectomy); 3.Ovariectomy; 4.Ovariectomy+E2; 5.Ovariectomy+sample1; 6.Ovariectomy+sample2; 7.Ovariectomy+sample3; 8.Ovariectomy+sample4

Target Data

Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Gene References into Functions
  1. These data suggest that Undercarboxylated osteocalcin may have other effects on skeletal muscle in addition to its insulin sensitizing effect. PMID: 27291707
  2. Uncarboxylated osteocalcin potentiates insulin secretion, inhibits Kv channels and increases [Ca(2+)]i. PMID: 27746193
  3. This rat model of complete loss of osteocalcin provides a platform for further understanding the role of osteocalcin in disease. PMID: 27483347
  4. gamma-Carboxylase inhibition prevents osteocalcin carboxylation and protects against diabetic cardiomyopathy, possibly through direct action on upregulated G protein-coupled receptor family C group 6 member A and indirectly via adiponectin. PMID: 27488359
  5. Bone Gla Protein PMID: 25185647
  6. In primary adipocytes, both carboxylated and uncarboxylated osteocalcin increased basal and insulin-stimulated glucose transport. PMID: 24554534
  7. The physiological changes associated with estrous cycle stage were not sufficient to cause a detectable difference in carboxylated or undercarboxylated osteocalcin concentrations in this study. PMID: 23817840
  8. Osteocalcin (OC)-containing neurons are distributed throughout the cerebellar nuclei; OC may play a role in modulating the neuroprotective function of osteopontin. PMID: 22552891
  9. warfarin impaired the carboxylation of osteocalcin in rats. Factor Xa inhibitor edoxaban at or higher doses than needed for an antithrombotic effect sustained the circulating Gla-osteocalcin level. PMID: 22999414
  10. results suggest that under some physiological conditions the oncoprotein MDM2 may cooperate with p53 to regulate the osteocalcin gene during osteoblastic differentiation. PMID: 22405968
  11. These data are strongly in support of Sp1 as an essential transcription factor required for Osx recruitment and transactivation of the OCN promoter. PMID: 21820092
  12. purified Bone Gla Protein (BGP) resulted in stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) in chondrocytes and vascular smooth muscle cells PMID: 21757657
  13. The mechanism of high glucose induced calcification in vascular smooth muscle cells may be due to the increased expression of cbfalpha-1 and OC. PMID: 21302441
  14. functional link between vitamin D-dependent bone tissue-specific transcription and histone acetylation at osteocalcin locus PMID: 11893738
  15. the VDRE in the distal region of the OC gene promoter is refractory to binding of the VDR-RXR complex when organized in a nucleosomal context PMID: 11964167
  16. deltaEF1 suppresses expression of the endogenous osteocalcin gene PMID: 12193549
  17. acetylation of histones H3 and H4 is functionally coupled to the chromatin remodeling events that mediate the developmental induction of osteocalcin gene transcription in bone cells PMID: 12554783
  18. Type I collagen promotes the activity of dentin formation by dental pulp cells by down-regulating Dmp-1 gene expression and up-regulating osteocalcin gene expression. PMID: 12647294
  19. A human vitamin D receptor-glucocorticoid receptor DNA-binding domain chimera is functional on rat osteocalcin vitamin D-responsive element with only conservative change of lys-49 to arg, and of negatively charged glu-53 to basic amino acid (lys or arg). PMID: 12960019
  20. Data show that in osteoblastic cells, the vitamin D receptor interacts directly with Runx2, and that this interaction contributes significantly to vitamin D3-dependent enhancement of the osteocalcin promoter. PMID: 15456860
  21. Results describe gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation. PMID: 15456894
  22. Ocn is a Nurr1 target gene, which positions Nurr1 in the core of transcriptional factors regulating osteoblastic gene expression PMID: 15485875
  23. OC-IR nerve fibers in the circumvallate papilla mainly originated from the petrosal ganglion. PMID: 15621021
  24. Osteonectin (ON) and osteocalcin (OC) was used as osteoblast markersand assessed using an indirect immunoperoxidase. technique. PMID: 16443258
  25. CEBP-beta-dependent recruitment of SWI/SNF activity is required for chromatin remodeling and transcriptional activity of the bone-specific osteocalcin gene PMID: 16772287
  26. specific protein-DNA and protein-protein interactions that occur within the context of the OC gene promoter in osteoblastic cells stabilize the preferential association of the VDR-SRC-1 complex PMID: 17218095
  27. These results support a model where VDR preferentially recruits SRC-1 to enhance bone-specific OC gene transcription. PMID: 17786964
  28. These data suggest that long-term reduction in bone OC levels may induce the formation of immature bone, which is easily resorbed with changes in bone metabolism such as ovariectomy, and that OC may be one of the factors affecting bone turnover. PMID: 17968486
  29. TFIIA gamma together with ATF4 and Runx2 stimulates osteocalcin promoter activity and endogenous mRNA expression. PMID: 18171674
  30. Biomineralization followed secretion of osteocalcin, which may reflect early osteoblastic differentiation of cultured mesenchymal stem cells under osteoinductive conditions. PMID: 19191495
  31. The Notch-responsive Hes-1 protein is capable of repressing osteocalcin gene transcription in osteoblastic cells through an E-box in the proximal promoter. PMID: 19670267

Show More

Hide All

Subcellular Location Secreted
Protein Families Osteocalcin/matrix Gla protein family
Database Links

KEGG: rno:25295

STRING: 10116.ENSRNOP00000026530

UniGene: Rn.9722

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1