Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Instructions
Code CSB-E05129r
Size 96T,5×96T,10×96T

Product Details

Target Name bone gamma-carboxyglutamate (gla) protein
Alternative Names RP11-54H19.5, BGP, OC, PMF1, bone Gla protein|bone gamma-carboxyglutamate (gla) protein (osteocalcin)|gamma-carboxyglutamic acid-containing protein|osteocalcin
Abbreviation BGLAP
Uniprot No. P04640
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 0.312 pg/mL-20 pg/mL
Sensitivity 0.078 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 100  
Range % 95-106  
1:400 Average % 89  
Range % 84-95  
1:800 Average % 88  
Range % 84-92  
1:1600 Average % 90  
Range % 85-95  
Recovery
The recovery of rat OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 83-90  
EDTA plasma (n=4) 104 99-109  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
20 2.306 2.368 2.337 2.152  
10 1.954 1.876 1.915 1.730  
5 1.583 1.469 1.526 1.341  
2.5 1.071 1.096 1.084 0.899  
1.25 0.705 0.748 0.727 0.542  
0.625 0.528 0.567 0.548 0.363  
0.312 0.317 0.302 0.310 0.125  
0 0.186 0.183 0.185    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Q&A
Q:

I would like to know what wavelength this product needs to be read at to make sure we have the appropriate filters. Also, we have some mouse cells lines, will this product work for those cell lines as well?

A:
Thanks for your inquiry.
We use TMB substrate. The detection wavelength must be 450nm. The absorbance value will be very good at this wavelength. Considering there may be scratches at the bottom of the vial when you add samples, we suggest you use dual wavelength( detection wavelength and correction wavelength).
We don't suggest you use this kit to test mouse cells lines. If you want to test it, you can do a pretest first.
 Customer Reviews

Target Data

Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Subcellular Location Secreted
Protein Families Osteocalcin/matrix Gla protein family
Database Links

KEGG: rno:25295

STRING: 10116.ENSRNOP00000026530

UniGene: Rn.9722

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.