Rat connective tissue growth factor,CTGF ELISA Kit

Instructions
Code CSB-E07876r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name connective tissue growth factor
Alternative Names Ccn2 ELISA Kit; CtgfCCN family member 2 ELISA Kit; Cellular communication network factor 2 ELISA Kit; Connective tissue growth factor ELISA Kit; Connective tissue growth-related protein ELISA Kit
Abbreviation CTGF
Uniprot No. Q9R1E9
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-96  
1:2 Average % 94  
Range % 91-98  
1:4 Average % 85  
Range % 89-100  
1:8 Average % 93  
Range % 86-99  
Recovery
The recovery of rat CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-96  
EDTA plasma (n=4) 96 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.097 1.987 2.042 1.880  
5 1.568 1.488 1.528 1.366  
2.5 1.007 1.001 1.004 0.842  
1.25 0.637 0.627 0.632 0.470  
0.625 0.370 0.360 0.365 0.203  
0.312 0.301 0.292 0.297 0.135  
0.156 0.244 0.239 0.242 0.080  
0 0.162 0.161 0.162    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).
Gene References into Functions
  1. Findings suggested that hypoxia induced CTGF upregulation in hypoxic pulmonary hypertension (HPH) rat model. CTGF mediated hypoxia-induced regulation of miR-26b and SRF in proliferation of pulmonary arterial smooth muscle cells, which indicated that hypoxia-induced miR-26b inhibition contributed to the pathogenesis of HPH via CTGF. PMID: 28816418
  2. Ang II down-regulates KLF15 expression via the AT1 receptor, and KLF15 is likely to inhibit Ang II-induced CTGF expression by repressing the recruitment of the co-activator P/CAF to the CTGF promoter. PMID: 29179208
  3. our findings showed that miR-375 binds to 3'-UTR region of Ctgf mRNA, subsequently leading to the decreased expression of Ctgf in the I/R brain. Furthermore, we showed that miR-375/Ctgf-mediated protective effects are associated with p21/PI3K/Akt signaling pathways. PMID: 29187583
  4. The blocking of this pathway by its inhibitor LY294002 impaired the proliferative effect of CCN2 on chondrocytes. These results suggested a novel physiological role of CCN2 in the development of condylar cartilage. PMID: 29198711
  5. CTGF may be a relevant factor contributing to tubulointerstitial fibrosis in polycystic kidney disease. PMID: 29058957
  6. Connective tissue growth factor (CTGF) has been found to be an important downstream mediator of TGF-beta1, and controls pro-fibrotic activities in renal fibrosis. PMID: 27597128
  7. the administration of cp-asiCTGF in the rat skin excision wound model efficiently reduced the induction of CTGF and collagens during the wound-healing process. These results suggest that the cp-asiCTGF molecule could be developed into antifibrotic therapeutics such as antiscar drugs PMID: 27427487
  8. Overexpression of KLF15 can partially rescue aortic remodeling and aortic dissection formation in animal models by inhibiting CTGF expression. PMID: 26600407
  9. Sitagliptin is a better renoprotective than Pioglitazone, probably due to its suppressor effect on CTGF, a key factor mediating diabetic renal injury PMID: 27049870
  10. RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix. PMID: 27126736
  11. In cultured neonatal cardiac fibroblasts, p63RhoGEF regulated the angiotensin II (Ang II)-dependent RhoA activation, the activation of the serum response factor, and the expression and secretion of CTGF. PMID: 26392029
  12. Ctgf stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat-induced pulmonary fibrosis. PMID: 25815693
  13. Over-expression of CTGF from a lentiviral vector increased the expression of Scx and Tnmd as well as tendon proteins type I collagen (ColI) and tenascin-C (Tn-C) in TSCs compared to non-treated control cells. PMID: 25833297
  14. Data indicate that connective tissue growth factor (CTGF) small hairpin RNA (pCTGF-shRNA) mediated by polyamidoamine reduced myocardial CTGF expression, suggesting that this system can be used to improve myocardial fibrosis therapy. PMID: 25177951
  15. Both the overexpression of CTGF and the loss of podocalyxin reflect renal damage in spontaneously hypertensive rats. CTGF and PCX may be involved in the mechanisms of podocyte injury and apoptosis induced by hypertension PMID: 25003484
  16. Our observations suggest a pathological role of CTGF in traumatic brain injury PMID: 25012526
  17. These findings indicated that CTGF plays a key role in the pathogenesis of liver fibrosis and lentiviral-mediated CTGF siRNA has the potential to be an effective treatment for liver fibrosis. PMID: 23456538
  18. Connective tissue growth factor and beta-catenin constitute an autocrine loop for activation in rat sarcomatoid mesothelioma. PMID: 24839947
  19. CTGF is critical in rat pulmonary injury induced by bleomycin PMID: 24083993
  20. CTGF mRNA expression increased progressively with the prolongation of Pseudomonas aeruginosa induced COPD. PMID: 23848567
  21. Connective tissue growth factor (CTGF/CCN2) is negatively regulated during neuron-glioblastoma interaction. PMID: 23383241
  22. Silencing CTGF and TIMP-1 caused decreased secretion of procollagen type-I, procollagen type-I and laminin in rat hepatic stellate cells. PMID: 23207149
  23. PPARgamma ligand shows potent inhibitory effect on TGF-beta1-induced CTGF expression in rat hepatic stellate cells. PMID: 19620052
  24. this study provides the first evidence for multi-organ regulation of CCN1 and CCN2 in early stages of sepsis, and implies the eruption of inflammatory mediators as a potential mechanism behind the observed CCN1 regulation. PMID: 22334618
  25. CTGF is expressed in mast cells in lungs in the development of pulmonary fibrosis. PMID: 22097728
  26. Rosiglitazone directly blocks the up-regulation of CTGF in pulmonary arteries of rats suffering from lung fibrosis. PMID: 20684283
  27. Baicallin could prevent the up-regulation of CTGF expression in fibrotic lungs of rats receiving bleomycin instillation. PMID: 21170500
  28. CTGF and alpha-SMA could play an important role in the development of pulmonary fibrosis caused by paraquat poisoning. PMID: 21126421
  29. JNK mediates the expression of CTGF and corneal scarring in corneal wound healing. PMID: 22363806
  30. Retinal cellular apoptosis index had a significant positive correlation to the expression of CTGF in diabetic rats. PMID: 21914267
  31. When the damaging effects on podocyte structure and function persist chronically, continuous CTGF expression in podocytes is a critical factor that promotes progressive accumulation of glomerular extracellular matrix and glomerulosclerosis. PMID: 21814837
  32. CTGF antagonism may offer a novel therapeutic strategy to alleviate BPD and PH in neonates. PMID: 21659659
  33. Atrial fibrillation patients and animals exhibited a significantly increased expression of connective tissue growth factor. Angiotension II-induced CTGF expression might be involved in atrial substrate remodeling. PMID: 21576830
  34. might play essential role in maintaining periodontal ligament fiber integrity under mechanical stress PMID: 21762408
  35. Intraportal vein siRNA injection targeting CTGF could significantly inhibit CTGF gene expression in rats, and thereby attenuate liver fibrosis by decreasing the number of active hepatic stellate cells. PMID: 21138629
  36. Upregulation of CTGF pulmonary gene expression after prenatal RA treatment may promote lung growth by promoting alveologenesis in the nitrofen-induced CDH model. PMID: 21258935
  37. IL-13 induces a time- and dosage-dependent increase of Connective tissue growth factor PMID: 21804025
  38. Rad GTPase inhibits cardiac fibrosis through connective tissue growth factor in failing hearts. PMID: 21382976
  39. Recombinant expression, purification, and functional characterisation of connective tissue growth factor and nephroblastoma-overexpressed protein PMID: 21209863
  40. In cardiac fibroblasts, Rac1 GTPase mediates upregulation of fibronectin via LOX and CTGF. PMID: 21215756
  41. CTGF mRNA expression in NRK52E cells was significantly associated with alpha-SMA mRNA expression. PMID: 20369462
  42. plasmid-based shRNA against CTGF attenuated pulmonary vascular remodeling in cigarette smoke-exposed rats PMID: 21295007
  43. Simvastatin can decrease the expression levels of CTGF and alpha-SMA in renal tubulointerstitium of rats with diabetic nephropathy. PMID: 20936727
  44. Glomerulosclerosis, tubulointerstitial fibrosis, and arterial media hypertrophy lesions of hypertensive nephrosclerosis are all characterized by increased CTGF tissue expression, which is associated with a concomitant increase in CTGF in blood and urine. PMID: 20689330
  45. These results suggest that the diabetes-mediated increase in CTGF upregulates VEGF and TGF-beta(2) expression and induces apoptosis in the retina. PMID: 20039857
  46. CTGF plays a significant role in the pathological changes of chronic allograft nephropathy after kidney transplantation. PMID: 20979869
  47. Upregulation of CTGF is associated with lung metastasis in chondrosarcoma. PMID: 20809981
  48. Trimetazidine effectively inhibits myocardial fibrosis, perhaps through the NADPH oxidase-ROS-CTGF signalling pathway. PMID: 20534773
  49. Decreasing expression of CTGF in ovary after chorionic gonadotropin treatment was time-dependent. PMID: 18846344
  50. The up-regulation of CTGF gene expression may play an important role in the development of pulmonary vascular remodeling in pulmonary hypertension. PMID: 19099753

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families CCN family
Database Links

KEGG: rno:64032

STRING: 10116.ENSRNOP00000020528

UniGene: Rn.17145

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