Rat inducible nitric oxide synthase,iNOS ELISA KIT

Instructions
Code CSB-E08325r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name nitric oxide synthase 2, inducible
Alternative Names Nos2 ELISA Kit; Nitric oxide synthase ELISA Kit; inducible ELISA Kit; EC 1.14.13.39 ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
Abbreviation NOS2
Uniprot No. Q06518
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 IU/mL-50 IU/mL
Sensitivity 0.195 IU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-100
1:2Average %94
Range %89-99
1:4Average %100
Range %95-104
1:8Average %102
Range %98-105
Recovery
The recovery of rat iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9189-95
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/ml. OD1OD2AverageCorrected
502.209 2.267 2.238 2.103
251.504 1.534 1.519 1.384
12.50.954 0.961 0.958 0.823
6.250.598 0.575 0.587 0.452
3.120.348 0.369 0.359 0.224
1.560.254 0.267 0.261 0.126
0.780.201 0.211 0.206 0.071
00.134 0.136 0.135  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
Gene References into Functions
  1. Early changes in impedance in response to I/R is related to histopathological changes, the nuclear stabilization and translocation of HIF-1alpha as well as expression of iNOS. PMID: 29451295
  2. A modulatory influence of the iNOS on the functional activity of the KATP-channels in coronary vessels of rats adapted to the stress has been reported. PMID: 30193054
  3. The results suggest that GPR30 activation inhibits myocardial fibrosis in diabetic ovariectomy female rats by suppressing cardiac iNOS activity and consequently nitric oxide levels. PMID: 30227089
  4. Study in surgery induced uremia rats suggest that chronic kidney disease could induce intestinal inflammation and lead to intestinal dysmotility, which may be associated with iNOS activation in the intestine. PMID: 29393230
  5. Gla treatment downregulated inducible nitric oxide synthase (iNOS) gene expression. PMID: 28707183
  6. Elevated TNF-alpha, nitric oxide (NO) and cytokines-IL-6 and IL-1beta with upregulated NF-kappaB/TNF-alpha/iNOS signalling pathways were effectively down-regulated by tangeretin. PMID: 28871498
  7. Expression of iNOS mRNA in the ischemic myocardial region increased significantly in the process of myocardial ischemic reperfusion injury. PMID: 29308569
  8. By down-regulation of common peripheral and local NFkappaB-iNOS pathway. PMID: 28009008
  9. findings suggest that TNF-alpha decreases mesenteric lymphatic contractility by activating the NF-kappaB-iNOS signaling pathway. PMID: 28231612
  10. In the dorsal raphe nucleus, iNOS plays a role in the anxiety-like behavior induced by ethanol withdrawal. PMID: 27139232
  11. AMPK activation upregulates cytokine-stimulated hepatocyte iNOS expression. PMID: 29229115
  12. HIgh fat diet increases testicular iNOS expression and this is not altered by antioxidants. PMID: 29023053
  13. Central MAPKs/PI3K/sGC pathways variably contribute to the TNF-alpha/iNOS-dependent reductions in blood pressure and heart rate variability seen during endotoxic shock. PMID: 27110744
  14. unpredictable chronic mild stress decreases penile constitutive eNOS and iNOS expression via TNF-alpha, which may contribute to the development of erectile dysfunction PMID: 27904147
  15. Our findings suggest that treatment with EPO or IPC and their combination improve renal function and preserve tubular damage induced by IR injury. These advantageous effects were closely related to reducing oxidative stress, suppressing proinflammatory response and enhancing generation of NO. IPC was more powerful in enhancement of eNOS gene expression compared to EPO that was more effective in increasing inos. PMID: 28471344
  16. Dopamin neurons exposed to preformed alpha-synuclein fibrils displayed a marked increase in iNOS protein levels compared to vehicle-treated neurons PMID: 28534083
  17. The findings suggest that shear stress upregulated AMPK phosphorylation in vascular smooth muscle cells via NOS expression may be a beneficial route to prevent pathogenesis in the vascular system. PMID: 28694192
  18. Both NOS I and NOS II are present in the thoracic spinal cord and are tonically active under physiological conditions. NO derived from NOS II exerts a tonic inhibitory action on sympathetic outflow from the sympathetic preganglionic neurons that targets primarily the blood vessels. PMID: 27371683
  19. Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the iNOS and PKG-1 signaling pathway. PMID: 26527422
  20. An animal model of chronic psychosocial stress, protected against hyper-production of iNOS in different brain structures. PMID: 27649096
  21. Treatment with etaftorone decreased expression of inducible NOS and XOR in kidneys, whereas it increased the expression of endothelial NOS. PMID: 26663724
  22. R59949, a diacylglycerol kinase inhibitor, inhibits iNOS nitric oxide production by decreasing transplasmalemmal L-arginine uptake in vascular smooth muscle cells. PMID: 27909743
  23. The results thus demonstrate that XO mediates oxidative stress in MB+PQ-treated rat PMNs via iNOS-independent but cytokine (predominantly IFN-gamma)-dependent mechanism. PMID: 28025796
  24. Folic acid/vitamin B12 may be possible nutritional intervention against nicotine-induced pancreatic islet cell damage by inhibiting inos and the generation of inflammation mediators. PMID: 27832994
  25. Immunosuppressive agents reduced expression of INOS/ENOS by coronary vessels to prevent transplant vasculopathy of cardiac allografts. PMID: 26924260
  26. Data suggest iNos plays role in pathogenesis (i.e., effects on other organs) of acute kidney injury, here due to ischemia/reperfusion or unilateral nephrectomy; specifically, iNos appears to play role in loss of function that occurs in contralateral kidney (but does not occur in lungs). PMID: 26854976
  27. injection of 10 mug/kg/12h progesterone hormone reduced the TNF-alpha and iNOS gene expression significantly and confirmed the role of progesterone in the reduction of inflammation. PMID: 27306339
  28. Review: inhibition of iNOS expression may be useful in preventing drug-induced liver injury. PMID: 27026409
  29. iNOS, but not prostaglandin E2, may play a primary role in delayed functional closure of the Ductus Arteriosus. PMID: 26821579
  30. The neonatal malnutrition impaired the animals' body weight. There was a lower expression of the inducible nitric oxide enzyme (iNOS), nitric oxide production, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus. PMID: 25701093
  31. Antioxidant diet in both genders is associated with less PAS-positive staining and less mesangial expansion than the regular diet, an early increase in cortical iNOS in males, and sex-specific changes in cortical eNOS at 20 weeks. PMID: 26797190
  32. These findings indicated that honokiol protects against renal ischemia/reperfusion injury via the suppression of oxidative stress, iNOS, inflammation and STAT3 in the rat. PMID: 26647858
  33. Overactivation of the adenosine system may foster iNOS-mediated diabetic vascular dysfunction. PMID: 26879172
  34. Combined stress increased iNOS expression, primarily in the CA3 area of the ventral hippocampus. PMID: 26608376
  35. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NFkappaB activation in HF rats. PMID: 26600052
  36. Up-regulation of y(+)LAT2 transporter is coupled with increased inducible nitric oxide synthase (iNOS) expression PMID: 26448619
  37. Data show that curcumin can recover learning and memory function through down-regulating hippocampal tumor necrosis factor alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS) levels. PMID: 26927554
  38. Cromoglycate ameliorated the injurious effect of warm ischemia/reperfusion in rat liver by inhibiting iNOS activity. PMID: 26396497
  39. The hearts from hypothyroid rats were resistant to Ischemia-reperfusion injury, partly due to the lower expression of iNOS and subsequent reduction in apoptosis after Ischemia-reperfusion. PMID: 26774797
  40. CAPE (Caffeic Acid Phenethyl Ester) affects expression of iNOS and cystathionine gamma-lyase in the hepatic fibrosis rat model PMID: 26378818
  41. While myocardial TLR4, MPO and ADMA levels increased in the MIR group, these parameters were found to be decreased significantly in the group treated with apocynin. PMID: 25404055
  42. HIF-1alpha may regulate the expression of iNOS and be involved in the damage of intestinal mucosa in hypobaric hypoxia. PMID: 26191216
  43. Inhibition of NOS-Nitric oxide system prevents autoimmune orchitis development in rats. PMID: 26046347
  44. Study showed that the mRNA expression of iNOS significantly increased in the group of experimental spinal cord injury, as compared with the sham-operated and the healthy control groups PMID: 25644387
  45. Data suggest that, in polycystic ovary syndrome, heart exhibits up-regulation of iNOS (nitric oxide synthase 2) activity, but down-regulation of both eNOS (nitric oxide synthase 3) and Na+/K+-ATPase (Na+/K+ transporting ATPase alpha 1) activities. PMID: 25988879
  46. Vitamin K1 attenuates NF-kappaB and iNOS expression in rat pancreas. PMID: 25466668
  47. Results indicate that inducible nitric oxide synthase (iNOS) expression within the ventrolateral medulla is differentially regulated by iNOS blockade that may, in part, contribute to the modulation of cardiovascular responses during static exercise. PMID: 25234194
  48. Results show that iNOS expression is dispensable to the course of tri-nitrobenzene sulphonic acid colitis, but is crucial in causing neural damage at early time points PMID: 25562655
  49. Our results demonstrated that ventricular hypertrophy abrogated isoflurane-induced delayed cardioprotection by alteration of iNOS/COX-2 pathway PMID: 25400168
  50. Profound cerebral artery vasodilation after traumatic brain injury is due to a gain of function in vascular NO production by 60-fold over controls, by iNOS induction in the endothelium. PMID: 25527626

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Protein Families NOS family
Tissue Specificity In normal kidney, expressed primarily in the medullary thick ascending limb, with minor amounts in the medullary collecting duct and vasa recta bundle.
Database Links

KEGG: rno:24599

STRING: 10116.ENSRNOP00000067662

UniGene: Rn.10400

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