Rat inducible nitric oxide synthase,iNOS ELISA KIT

Code CSB-E08325r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name nitric oxide synthase 2, inducible
Alternative Names Nos2 ELISA Kit; Nitric oxide synthase ELISA Kit; inducible ELISA Kit; EC ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
Abbreviation NOS2
Uniprot No. Q06518
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 IU/mL-50 IU/mL
Sensitivity 0.195 IU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %96
Range %90-100
1:2Average %94
Range %89-99
1:4Average %100
Range %95-104
1:8Average %102
Range %98-105
The recovery of rat iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9189-95
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/ml. OD1OD2AverageCorrected
502.209 2.267 2.238 2.103
251.504 1.534 1.519 1.384
12.50.954 0.961 0.958 0.823
6.250.598 0.575 0.587 0.452
3.120.348 0.369 0.359 0.224
1.560.254 0.267 0.261 0.126
0.780.201 0.211 0.206 0.071
00.134 0.136 0.135  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Target Data

Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
Gene References into Functions
  1. Early changes in impedance in response to I/R is related to histopathological changes, the nuclear stabilization and translocation of HIF-1alpha as well as expression of iNOS. PMID: 29451295
  2. A modulatory influence of the iNOS on the functional activity of the KATP-channels in coronary vessels of rats adapted to the stress has been reported. PMID: 30193054
  3. The results suggest that GPR30 activation inhibits myocardial fibrosis in diabetic ovariectomy female rats by suppressing cardiac iNOS activity and consequently nitric oxide levels. PMID: 30227089
  4. Study in surgery induced uremia rats suggest that chronic kidney disease could induce intestinal inflammation and lead to intestinal dysmotility, which may be associated with iNOS activation in the intestine. PMID: 29393230
  5. Gla treatment downregulated inducible nitric oxide synthase (iNOS) gene expression. PMID: 28707183
  6. Elevated TNF-alpha, nitric oxide (NO) and cytokines-IL-6 and IL-1beta with upregulated NF-kappaB/TNF-alpha/iNOS signalling pathways were effectively down-regulated by tangeretin. PMID: 28871498
  7. Expression of iNOS mRNA in the ischemic myocardial region increased significantly in the process of myocardial ischemic reperfusion injury. PMID: 29308569
  8. By down-regulation of common peripheral and local NFkappaB-iNOS pathway. PMID: 28009008
  9. findings suggest that TNF-alpha decreases mesenteric lymphatic contractility by activating the NF-kappaB-iNOS signaling pathway. PMID: 28231612
  10. In the dorsal raphe nucleus, iNOS plays a role in the anxiety-like behavior induced by ethanol withdrawal. PMID: 27139232
  11. AMPK activation upregulates cytokine-stimulated hepatocyte iNOS expression. PMID: 29229115
  12. HIgh fat diet increases testicular iNOS expression and this is not altered by antioxidants. PMID: 29023053
  13. Central MAPKs/PI3K/sGC pathways variably contribute to the TNF-alpha/iNOS-dependent reductions in blood pressure and heart rate variability seen during endotoxic shock. PMID: 27110744
  14. unpredictable chronic mild stress decreases penile constitutive eNOS and iNOS expression via TNF-alpha, which may contribute to the development of erectile dysfunction PMID: 27904147
  15. Our findings suggest that treatment with EPO or IPC and their combination improve renal function and preserve tubular damage induced by IR injury. These advantageous effects were closely related to reducing oxidative stress, suppressing proinflammatory response and enhancing generation of NO. IPC was more powerful in enhancement of eNOS gene expression compared to EPO that was more effective in increasing inos. PMID: 28471344
  16. Dopamin neurons exposed to preformed alpha-synuclein fibrils displayed a marked increase in iNOS protein levels compared to vehicle-treated neurons PMID: 28534083
  17. The findings suggest that shear stress upregulated AMPK phosphorylation in vascular smooth muscle cells via NOS expression may be a beneficial route to prevent pathogenesis in the vascular system. PMID: 28694192
  18. Both NOS I and NOS II are present in the thoracic spinal cord and are tonically active under physiological conditions. NO derived from NOS II exerts a tonic inhibitory action on sympathetic outflow from the sympathetic preganglionic neurons that targets primarily the blood vessels. PMID: 27371683
  19. Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the iNOS and PKG-1 signaling pathway. PMID: 26527422
  20. An animal model of chronic psychosocial stress, protected against hyper-production of iNOS in different brain structures. PMID: 27649096
  21. Treatment with etaftorone decreased expression of inducible NOS and XOR in kidneys, whereas it increased the expression of endothelial NOS. PMID: 26663724
  22. R59949, a diacylglycerol kinase inhibitor, inhibits iNOS nitric oxide production by decreasing transplasmalemmal L-arginine uptake in vascular smooth muscle cells. PMID: 27909743
  23. The results thus demonstrate that XO mediates oxidative stress in MB+PQ-treated rat PMNs via iNOS-independent but cytokine (predominantly IFN-gamma)-dependent mechanism. PMID: 28025796
  24. Folic acid/vitamin B12 may be possible nutritional intervention against nicotine-induced pancreatic islet cell damage by inhibiting inos and the generation of inflammation mediators. PMID: 27832994
  25. Immunosuppressive agents reduced expression of INOS/ENOS by coronary vessels to prevent transplant vasculopathy of cardiac allografts. PMID: 26924260
  26. Data suggest iNos plays role in pathogenesis (i.e., effects on other organs) of acute kidney injury, here due to ischemia/reperfusion or unilateral nephrectomy; specifically, iNos appears to play role in loss of function that occurs in contralateral kidney (but does not occur in lungs). PMID: 26854976
  27. injection of 10 mug/kg/12h progesterone hormone reduced the TNF-alpha and iNOS gene expression significantly and confirmed the role of progesterone in the reduction of inflammation. PMID: 27306339
  28. Review: inhibition of iNOS expression may be useful in preventing drug-induced liver injury. PMID: 27026409
  29. iNOS, but not prostaglandin E2, may play a primary role in delayed functional closure of the Ductus Arteriosus. PMID: 26821579
  30. The neonatal malnutrition impaired the animals' body weight. There was a lower expression of the inducible nitric oxide enzyme (iNOS), nitric oxide production, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus. PMID: 25701093
  31. Antioxidant diet in both genders is associated with less PAS-positive staining and less mesangial expansion than the regular diet, an early increase in cortical iNOS in males, and sex-specific changes in cortical eNOS at 20 weeks. PMID: 26797190
  32. These findings indicated that honokiol protects against renal ischemia/reperfusion injury via the suppression of oxidative stress, iNOS, inflammation and STAT3 in the rat. PMID: 26647858
  33. Overactivation of the adenosine system may foster iNOS-mediated diabetic vascular dysfunction. PMID: 26879172
  34. Combined stress increased iNOS expression, primarily in the CA3 area of the ventral hippocampus. PMID: 26608376
  35. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NFkappaB activation in HF rats. PMID: 26600052
  36. Up-regulation of y(+)LAT2 transporter is coupled with increased inducible nitric oxide synthase (iNOS) expression PMID: 26448619
  37. Data show that curcumin can recover learning and memory function through down-regulating hippocampal tumor necrosis factor alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS) levels. PMID: 26927554
  38. Cromoglycate ameliorated the injurious effect of warm ischemia/reperfusion in rat liver by inhibiting iNOS activity. PMID: 26396497
  39. The hearts from hypothyroid rats were resistant to Ischemia-reperfusion injury, partly due to the lower expression of iNOS and subsequent reduction in apoptosis after Ischemia-reperfusion. PMID: 26774797
  40. CAPE (Caffeic Acid Phenethyl Ester) affects expression of iNOS and cystathionine gamma-lyase in the hepatic fibrosis rat model PMID: 26378818
  41. While myocardial TLR4, MPO and ADMA levels increased in the MIR group, these parameters were found to be decreased significantly in the group treated with apocynin. PMID: 25404055
  42. HIF-1alpha may regulate the expression of iNOS and be involved in the damage of intestinal mucosa in hypobaric hypoxia. PMID: 26191216
  43. Inhibition of NOS-Nitric oxide system prevents autoimmune orchitis development in rats. PMID: 26046347
  44. Study showed that the mRNA expression of iNOS significantly increased in the group of experimental spinal cord injury, as compared with the sham-operated and the healthy control groups PMID: 25644387
  45. Data suggest that, in polycystic ovary syndrome, heart exhibits up-regulation of iNOS (nitric oxide synthase 2) activity, but down-regulation of both eNOS (nitric oxide synthase 3) and Na+/K+-ATPase (Na+/K+ transporting ATPase alpha 1) activities. PMID: 25988879
  46. Vitamin K1 attenuates NF-kappaB and iNOS expression in rat pancreas. PMID: 25466668
  47. Results indicate that inducible nitric oxide synthase (iNOS) expression within the ventrolateral medulla is differentially regulated by iNOS blockade that may, in part, contribute to the modulation of cardiovascular responses during static exercise. PMID: 25234194
  48. Results show that iNOS expression is dispensable to the course of tri-nitrobenzene sulphonic acid colitis, but is crucial in causing neural damage at early time points PMID: 25562655
  49. Our results demonstrated that ventricular hypertrophy abrogated isoflurane-induced delayed cardioprotection by alteration of iNOS/COX-2 pathway PMID: 25400168
  50. Profound cerebral artery vasodilation after traumatic brain injury is due to a gain of function in vascular NO production by 60-fold over controls, by iNOS induction in the endothelium. PMID: 25527626
  51. Intracellular signaling pathways mediated by NO and ROS are linked to each other via mtROS and form an iNOS-mtROS feed-forward loop which aggravates liver failure on acute inflammation. PMID: 25365698
  52. polysaccharide components in pu-erh tea reduce NO levels in an animal model by inhibiting the iNOS expression via signaling through TLR4 PMID: 22837686
  53. iNOS upregulation contributes to liver microvascular dysfunction in endotoxemia. PMID: 25038487
  54. the photoperiod affects the diurnal expression of iNOS and activation of CREB in the hippocampus, which provide clues for the possible impact of circadian changes in hippocampal functions. PMID: 25687488
  55. Data show that palmitic acid significantly stimulated mRNA and protein expression of C-reactive protein, inducible nitric oxide synthase and tumor necrosis factor-alpha in vascular smooth muscle cells in time- and concentration-dependent manners. PMID: 25242580
  56. Regular exposure to low altered the level of iNOS expression in the myocardium, rendering the heart more sensitive to cardiac stress such as I/R. PMID: 25595132
  57. The inhibition of NOS, but not HO, accounts for the baroreflex depressant of chronic nicotine. PMID: 24870610
  58. The plantaris of heart failure rats displayed atrophy associated with increased Nox2 mRNA. PMID: 25023789
  59. Convention ischemic preconditiong, but not remote-IPC, reduces iNOS and IL-1 receptor transcription during early reperfusion, ameliorating ischemia reperfusion injury. PMID: 25071345
  60. Expression of inducible nitric oxide synthase (iNOS) was detected. PMID: 24768712
  61. Mangosteen pericarp ethanolic extract inhibits iNOS signaling to decrease vasa vasorum angiogenesis in hypercholesterolemic rats. PMID: 25187725
  62. Inducible nitric oxide synthase inhibition reverses pulmonary arterial dysfunction in lung transplantation PMID: 24760104
  63. Increased iNOS expression can lead to osteocyte apopotosis in idiopathic, non-traumatic osteonecrosis. PMID: 25191931
  64. Calcium-mediated signaling regulates hepatocyte iNOS expression and does so through a mechanism independent of calcineurin. PMID: 25150084
  65. Suggest that iNOS plays a dominant role in liver ischemia and reperfusion-induced transient increase in arterial stiffening. PMID: 24815141
  66. iNOS inhibitor 1,400W was effective in reducing myocardial ischemia/reperfusion injury-induced hemodynamic instability and kidney injury. PMID: 24815143
  67. In some cases it can be a source of a large number of NO (coupling state of iNOS), and in another contribute reduce its bioavailability (uncoupling state of iNOS). PMID: 25470921
  68. Data indicate that triptolide showed a good analgesic effect on adjuvant arthritis (AA) and could inhibit the inducible nitric oxide synthase (iNOS) and substance P (SP) expressions in spinal dorsal horn and dorsal root ganglion in AA. PMID: 25095383
  69. The special role of inducible NO-synthase in the mechanism of rat coronary vessels tone regulation during immobilization stress, combined with diabetes mellitus PMID: 25272454
  70. Regardless of age the level of iNOS mRNA in the retina of the senescence-accelerated OXYS rats was 7 times lower than that in Wistar rats, but the protein content of iNOS in OXYS rats (ELISA data) was increased. PMID: 25051763
  71. Epigallocatechin gallate attenuated hepatic changes including fibrosis with down-regulation in the expressions of key pathological oxidative and pro-inflammatory markers (e.g. iNOS, COX-2, and TNF-alpha). PMID: 23515587
  72. In conclusion, B. trimera extracts exert antioxidant properties by scavenging ROS and decrease the expression of genes responsible by reactive species production in neutrophils. PMID: 24372242
  73. HSP70 protects against apoptotic cell death induced by NF-kappaB activation and NOS II-peroxynitrite signaling cascade in the hippocampal CA3 PMID: 24141017
  74. baicalin could ameliorate LPS-induced iNOS and NO overproduction in mucosa of rat terminal ileum via inhibition of p38 signalling cascade and activation of PPARgamma pathway. PMID: 24312512
  75. The radioprotective effect may be directly related to effective attenuation of the radiation-induced elevation of NO production by L-NAME and of oxidative stress by ALC. PMID: 23869893
  76. Loss of transplanted bone marrow stem cells in the infarcted myocardium is due to increased apoptosis and iNOS overexpression. PMID: 24780316
  77. Dietary high-fructose corn syrup causes vascular insulin resistance and endothelial dysfunction through attenuating IRS-1 and eNOS expressions as well as increasing iNOS in rats. PMID: 23872130
  78. increased iNOS activity contributes to ischemia reperfusion injury and acute graft rejection. PMID: 23953569
  79. C-peptide suppresses iNOS expression in rat mesangial cells. PMID: 23973463
  80. RELA/p65 and STAT1 cooperate to control iNOS gene transcription in response to proinflammatory cytokines by a coactivator exchange mechanism. PMID: 24014650
  81. Increased levels of iNOS are present in the in the colonic surface epithelium inducing increased mucus thickness in a rat model of ulcerative colitis. PMID: 23977158
  82. After both CdCl2 and silica nanoparticles doped with cadmium treatments, pulmonary over-expression of SOD1, COX-2 and iNOS extensively spread out in bronchiolar and alveolar cells, as well as in the capillary component of lung tissue of rats. PMID: 22898625
  83. Data show that the CB1 and CB2 receptor agonists attenuate iNOS and ROS generation in microglia due to their reduction of phosphorylation of extracellular signal regulated kinase 1/2 (ERK1/2), cytosolic phospholipase A (cPLA) and activation of NF-kappaB. PMID: 23219970
  84. biliary drainage affects expression of inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats PMID: 23613625
  85. Lower activity and expression levels of iNOS and higher activity and expression levels of arginase-1 in rat alveolar macrophages were found to be linked to the susceptibility of T. gondii infection in these cells PMID: 23691079
  86. iNOS expression is highly modulated in alveolar epithelial cells by LPS via a Ca(2)/PKCalpha-beta1 pathway independent of ATP signaling. PMID: 23686852
  87. MSU-induced inflammatory cell infiltration, as well as the elevated expression of c-Fos and inducible nitric oxide synthase and cyclooxygenase-2 observed in synovial tissue, were significantly inhibited by treatment with lemnalol. PMID: 23306170
  88. The cardioprotection afforded by sabiporide is attributed in part to inhibition of ERK1/2 phosphorylation and suppression of iNOS expression. PMID: 23484128
  89. study demonstrates that miR-145 regulates reactive oxygen species (ROS)-induced Ca(2+) overload in cardiomyocytes. PMID: 23702479
  90. Report role of brain iNOS in regulating Il-1 beta activation of the hypothalamic-pituitary-adrenal axis. PMID: 23406756
  91. Our results suggest a novel signaling pathway in beta-cells in which p38 MAPK promotes translation elongation of Nos2 mRNA via regulation of eIF5A hypusination. PMID: 23250488
  92. iNOS plays an important part in burn injury-induced GSK-3beta activation in skeletal muscle. PMID: 22995863
  93. The presence of oxidized low-density lipoproteins, superoxide dismutase enzyme activity, malondialdehyde levels, and iNOS expression were evaluated, after ischemia reperfusion. PMID: 22226057
  94. Puerarin can decrease retinal pigment epithelial cell apoptosis in diabetic rats by reducing peroxynitrite level and iNOS expression. PMID: 22513471
  95. Quercetin/quercitrin reduced expression of iNOS and inhibited translocation of NF-kappaB in rat insulinoma cells. PMID: 23138875
  96. Sevoflurane preconditioning produces delayed cardioprotection through the up-regulation of iNOS expression. PMID: 23086649
  97. Comparison of NOS isoforms' levels in the hippocampi of "good" and "poor" learners between the two strains confirmed significantly higher level of iNOS. PMID: 22909987
  98. Icariside II stimulates osteogenic differentiation of stromal cells via enhancing the activity of iNOS and promoting the generation of NO. PMID: 21751490
  99. NOS2 in the spinal cord may mediate withdrawal syndrome in morphine-dependent rats. PMID: 22493895
  100. The hypoxia-sensitive transcription factor HIF-1alpha and iNOS appear to play a pivotal role in the delayed pharmacological myocardial preconditioning induced by cobalt. PMID: 21480981

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Protein Families NOS family
Tissue Specificity In normal kidney, expressed primarily in the medullary thick ascending limb, with minor amounts in the medullary collecting duct and vasa recta bundle.
Database Links

KEGG: rno:24599

STRING: 10116.ENSRNOP00000067662

UniGene: Rn.10400

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