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| Code | CSB-E08005r |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
This Rat Tissue Inhibitors of Metalloproteinase 1 (TIMP-1) ELISA Kit is an accurate and reliable solution for measuring TIMP-1 levels in serum, plasma, cell culture supernates, and tissue homogenates of Rattus norvegicus (Rat). This ELISA kit has been specifically designed to detect and quantify TIMP-1 levels, an important protein involved in regulating matrix metalloproteinase activity, which is a key process in cardiovascular research.Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades. With a detection range of 0.156 ng/mL to 10 ng/mL and a sensitivity of 0.224 ng/mL, this quantitative assay can provide precise and reproducible results in just 1 to 5 hours. The sample volume required is only 50-100ul, making it an economical and efficient option for researchers. The assay principle is based on a sandwich ELISA method, which utilizes specific antibodies to capture and detect TIMP-1 in the sample. The detection wavelength is 450 nm, ensuring accurate and reliable measurements. |
| Target Name | TIMP metallopeptidase inhibitor 1 |
| Alternative Names | Timp1 ELISA Kit; Timp-1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit |
| Abbreviation | TIMP1 |
| Uniprot No. | P30120 |
| Species | Rattus norvegicus (Rat) |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 0.156 ng/mL-10 ng/mL |
| Sensitivity | 0.224 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision |
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. |
| Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of Rat TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
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| Function (From Uniprot) |
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity.
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| Gene References into Functions |
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| Subcellular Location | Secreted. |
| Protein Families | Protease inhibitor I35 (TIMP) family |
| Database Links |
KEGG: rno:116510 STRING: 10116.ENSRNOP00000013745 UniGene: Rn.25754 |
