Name: Rat tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E07386r

Product Details

Description

This Rat TIMP2 ELISA Kit was designed for the quantitative measurement of Rat TIMP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.

Target Name

TIMP metallopeptidase inhibitor 2

Alternative Names

Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit

Abbreviation

TIMP2

Uniprot No.

P30121

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 ng/mL-80 ng/mL

Sensitivity

0.31 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	97
	Range %	94-103
1:8	Average %	101
	Range %	96-105

Recovery

The recovery of rat TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	94-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.184	2.189	2.187	2.009
40	1.486	1.456	1.471	1.293
20	0.925	0.994	0.960	0.782
10	0.545	0.588	0.567	0.389
5	0.431	0.443	0.437	0.259
2.5	0.308	0.292	0.300	0.122
1.25	0.251	0.233	0.242	0.064
0	0.179	0.177	0.178

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Customer Reviews and Q&A

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■ Q&A

Can you please provide the following information about this kit:
a. Capture antibody or capture antigen.
b. Detection antibody (if applicable).
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
4) If you have data on the normal value and the value in case of renal failure of TIMP-2 in rats with the kit, please send me too.
5) Please send the current manual.

Thanks for your inquiry.
CSB-E07386r
This kit is designed against full length protein. Pls refer to: https://www.uniprot.org/uniprot/P30121.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is NS0-derived recombinant protein.
We tested undiluted serum and plasma samples before and the test value is 2.5--10ng/ml.
I will send you the manual by email.

Target Background

Function (From Uniprot)	Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Gene References into Functions	Dynamics of distribution of capillaries with MMP2 and TIMP2 expressed in rat brain during development of experimental hypertension has been reported. PMID: 29308554 During the processes of conjunctival filtering bleb and scar formation in rats, the changes in MMP-2 and TIMP-2 levels in the filtering area, together with a corresponding proliferation of fibroblasts and the accumulation of collagen fibres, resulted in scarring of filtering blebs. PMID: 29075637 These findings suggested that artesunate attenuates alveolitis and pulmonary fibrosis by regulating expression of collagen-IV, TIMP-1 and 2, as well as MMP-2 and -9, to reduce ECM accumulation. PMID: 27323108 Data suggest that amlodipine and Atorvastatin could improve ventricular remodeling in SHR rats through intervention with the imbalance of MMP-2/TIMP-2 and MMP-9/TIMP-1 system. PMID: 27322513 decreased in diabetic skin PMID: 26047379 Lung tissue from burn animals showed increased matrix metalloproteinase activity, decreased levels of TIMP-2, and no difference in levels of active MMP-9 in burn vs. control groups. PMID: 26601648 MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression were significantly reduced in the lateral prostatic lobe of the ethanol drinking animals. PMID: 26258010 The present study suggests that EPO can exert a cardioprotective effect on DOX-induced cardiotoxicity which may be associated with improving MMP-2/TIMP-2 imbalance. PMID: 24685074 Enhancement of TIMP-2 expression may be a potential therapeutic target for neuroinflammatory disorders. PMID: 24970341 Bone marrow mononuclear cell transplantation significantly increased MMP-9 and MMP-13, and decreased TIMP expression. PMID: 23886643 the expression of TIMP-2 increased only in the neutrophils, endotheliocytes, and fibroblasts, remaining to be equal over all stages. PMID: 23383440 Membrane type 1 MMP, MMP2, and TIMP2 were present in testis during gonocyte migration and transformation into spermatogenic stem cells. PMID: 22974613 Inulicin inhibits hyperplasia of neointima by modulating the balance of MMP-2 and TIMP-2. PMID: 21141515 Data show that MMPs 2 and 9 and their inhibitors, TIMPs 1 and 2, are upregulated in response to oxidative stress produced by HIV-1 gp120 in a rat model of HIV encephalopathy. PMID: 22092673 NDRG2 contributed to an increase in the ratio of matrix metalloproteinase 2 (MMP2) to tissue inhibitor of matrix metalloproteinase 2 PMID: 22110735 Trained spontaneously hypertensive rats showed higher metalloproteinase-2/tissue inhibitor metalloproteinase-2 (MMP-2/TIMP-2) ratio and lower levels of alpha-smooth muscle actin. PMID: 22045123 A dramatic increase of TIMP1 mRNA and a decrease of TIMP2 in DRG after sciatic nerve transection. PMID: 21782897 the present study showed for the first time the mode of TIMP-1 and -2 expression and activity in a rat liver fibrosis model. PMID: 20226641 After transfection with FRNK, MMP-2 was up-regulated and TIMP-2 was down-regulated in hepatic stellate cells. PMID: 18983773 The TIMP-2 gene mediated by adenovirus can renew the balance of degradation of the extracellular matrix caused by elastase and inhibit the formation of an abdominal aortic aneurysm. PMID: 20103929 Addition of follicle stimulating hormone to Sertoli cells increased the TIMP-2 mRNA level three-fold, implicating a role in spermatogenesis. PMID: 11928819 Data show that matrix metalloproteinase-2 and TIMP-2 are likely involved in the FSH-induced changes in Sertoli cells. PMID: 12039062 tissue inhibitor of metalloproteinase-2 mRNA was observed surrounding the developing corpus luteum and was present in granulosa-lutein cells, with high levels near the center of the corpus luteum PMID: 12444073 results suggest that the migration and invasion of cardiac fibroblasts is furin-dependent and that the active species of MT1-MMP and MMP-2 may be involved in both events PMID: 12970340 In the process of hepatic fibrosis, fibroblasts and myofibroblasts are the major cells that express TIMPs and the more serious the hepatic fibrosis is in the injured liver, the higher the level of TIMP-1 and TIMP-2 gene expression. PMID: 14695775 Blood leukocytes and loss of flow are major factors in the activation of MMP-2, and cytokine-mediated differential regulation of TIMP-1 and TIMP-2 may contribute significantly to blood brain barrier failure. PMID: 16707552 MMP2 plays a role in the development of BC metastases. Furthermore, our results suggest that astrocyte factors and the ERK1/2 signaling pathway may be associated with BC brain metastasis development. PMID: 17505812 matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-1 and osteopontin have roles in heart failure in DOCA-salt hypertensive rat PMID: 18415800 TIMP-2 is an osteoanabolic factor in vitro and in vivo PMID: 18558556 The induction of MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNAs post-castration could be at least in part due to post-transcriptional stabilization. PMID: 18675881 investigated the involvement of matrix metalloproteinases (MMPs), tissue inhibitor (TIMP) and endothelin-1 (ET-1) in the renal damage in spontaneously hypertensive rats following nitric oxide deprivation. PMID: 18935914 over-expressed ZAK may contribute to the progression of myocardial remodeling by disrupting the balance in the MMP-2/9 and TIMP-1/2 expression level, respectively PMID: 19184368 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Protease inhibitor I35 (TIMP) family
Database Links	KEGG: rno:29543 STRING: 10116.ENSRNOP00000004290 UniGene: Rn.10161

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Code	CSB-E07386r
Size	96T,5×96T,10×96T
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Rat tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit

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