Human versican/PG-M/PG-350 ELISA kit

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Target Background

Name: Human versican/PG-M/PG-350 ELISA kit
Brand: CUSABIO
SKU: CSB-E11884h

Product Details

Alternative Names

Chondroitin sulfate proteoglycan 2 ELISA Kit; Chondroitin sulfate proteoglycan core protein 2 ELISA Kit; Chondroitin sulfate proteoglycan core protein; cartilage ELISA Kit; CSPG2 ELISA Kit; CSPG2_HUMAN ELISA Kit; ERVR ELISA Kit; GHAP ELISA Kit; Glial hyaluronate binding protein ELISA Kit; Glial hyaluronate-binding protein ELISA Kit; Large fibroblast proteoglycan ELISA Kit; PG-M ELISA Kit; PGM ELISA Kit; VCAN ELISA Kit; Versican ELISA Kit; Versican core protein ELISA Kit; Versican proteoglycan ELISA Kit; WGN 1 ELISA Kit; WGN ELISA Kit; WGN1 ELISA Kit

Abbreviation

VCAN

Uniprot No.

P13611

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates.

Detection Range

0.04 ng/ml-30 ng/ml.

Sensitivity

0.04 ng/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human versican/PG-M/PG-350 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	90
	Range %	85-95
1:40	Average %	97
	Range %	91-105
1:80	Average %	92
	Range %	88-97
1:160	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.803

2.734

2.768

2.595

2.365

2.367

2.366

2.193

3.33

1.805

1.828

1.816

1.644

1.11

1.114

1.129

1.121

0.948

0.37

0.673

0.651

0.662

0.489

0.12

0.384

0.363

0.373

0.200

0.04

0.265

0.274

0.269

0.096

0.171

0.175

0.173

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The human VCAN ELISA kit (CSB-E11884h) is designed for the quantitative measurement of human VCAN protein in samples. It shows excellent specificity for human VCAN. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated VCAN antibody that recognizes the analyte bound by the immobilized VCAN antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of VCAN in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the human VCAN in the sample.

VCAN is a large extracellular matrix (ECM) proteoglycan that is significantly increased expressed and accumulated during inflammation in most diseases. It plays an essential role during development and is an important component of tissue inflammation caused by infection and tissue injury. VCAN interacts with its receptors such as CD44, PSGL-1, and TLR2, initiating a signaling cascade that affects the phenotypes of immune and inflammatory cells. It is also implicated in multiple processes of cancer development, including proliferative signaling, evasion of growth-suppressor pathways, modulation of cell death, promotion of neoangiogenesis, and tissue invasion & metastasis.

Target Background

Function
(From Uniprot)

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

Gene References into Functions

VCAN expression in colorectal cancer and its role in cetuximab resistance PMID: 28320945
miR-135a-5p can suppress cell progress including cell proliferation, migration, and invasion in thyroid carcinoma by targeting VCAN 3 '-UTR. PMID: 28869452
This study highlights the oncogenic role of VCAN in renal cell carcinogenesis and suggests that this gene has therapeutic and/or biomarker potential for renal cell cancer. PMID: 28242813
Interleukin-17A promotes tongue squamous cell carcinoma metastasis by activating miR-23b/versican pathway in the tumor microenvironment. PMID: 28035060
The macular dysfunction on mfERG was profound and of early onset. A heterozygous mutation in intron 7 of the VCAN gene (c.4004-1G > A) was found. PMID: 29071374
Versican V0 and V1 isoforms were upregulated in the uterine leiomyomas of symptomatic versus asymptomatic women. Abundant cleaved versican was detected in leiomyoma and myometrium, as well as in myometrial and leiomyoma cell lines. VCAN siRNA did not effect cell proliferation, apoptosis, or smooth muscle markers, but reduced ESR1 and PR-A expression. PMID: 28323982
Lumican and versican protein expression are associated with colorectal adenoma-to-carcinoma progression PMID: 28481899
Removal or knockdown of versican may be a possible therapeutic strategy for increasing deposition of insoluble elastin and stimulating repair of elastic fibers in COPD lung. PMID: 28138236
human myeloma tumors displaying CD8(+) infiltration/aggregates underwent VCAN proteolysis at a site predicted to generate a glycosaminoglycan-bereft N-terminal fragment. PMID: 27259980
versican decreases in follicular dermal papilla as an aging-associated change of human hair follicles PMID: 27697422
Data indicate that serum versican levels were significantly decreased in polycystic ovary syndrome (PCOS) patients, and that serum ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin motif-1) and versican levels were significantly and positively correlated with each other. PMID: 27908842
the G1 domain of versican can regulate the organization of pericellular hyaluronan and affect phenotype of cultured human dermal fibroblasts PMID: 27126822
Results highlight an important role for versican in regulating the expression and assembly of elastin and the phenotype of leiomyosarcoma cells. PMID: 26723257
Versican localizes to the nucleus in proliferating mesenchymal cells and suggest role in mitotic spindle organization during cell division. PMID: 26395512
VCAN and VEGF were associated with survival in CRC patients with PM after CRS and HIPEC PMID: 26873137
Versican is a novel modulator of hepatic fibrosis. PMID: 26752747
versican mRNA is up-regulated much more highly (>600 fold) by long term hypoxia (5 days) than by 1 day of hypoxia. PMID: 26057378
Versican V1 overexpression induces a myofibroblast-like phenotype in cultured fibroblasts. PMID: 26176948
Versican upregulation in Sezary cells alters growth, motility and resistance to chemotherapy. PMID: 25915825
Versican isoform V1 is associated with high-grade gliomas. PMID: 25064688
Significant elevation of VCAN and its associated molecules imply their role in multiple myeloma PMID: 25623955
Our results suggest that TGF-beta1 up-regulates versican expression by suppressing miR-143, and this pathway is important for osteosarcoma cell migration and invasion. PMID: 25562163
Circulating monocytes may contribute to the fibrotic process in a subset of systemic sclerosis patients by amplifying a positive feedback loop consisting of versican, CCL2, and the influx of monocytes PMID: 23845159
Arylsulfatase B regulates versican expression by galectin-3 and AP-1 mediated transcriptional effects. PMID: 24240681
Versican regulates the growth of leiomyosarcoma tumors. PMID: 25320080
these data suggest that versican regulates the development of peritoneal metastasis originating from cells and spheroids. PMID: 24999371
The ADAMTS5 ancillary domain and specific chondroitin sulfate chains of versican are required for proteolysis. PMID: 25122765
Versican expression in tumor epithelial cells predicts a good prognosis for gastric cancer patients. PMID: 25275064
Lack of versican expression is associated with recurrence in colon cancer. PMID: 22711178
CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion. PMID: 24180670
FoxQ1 promotes hepatocellular carcinoma metastasis by transactivating ZEB2 and VersicanV1 expression. PMID: 24005989
A novel c.4004-6T>A nucleotide substitution at the acceptor splice site of intron 7 of the VCAN gene that segregated with the Wagner disease phenotype, was identified. PMID: 24174867
Cleaved versican G1 domain-containing fragments (VG1F) can be recaptured by microfibrils through VG1F homotypical interactions to enhance hyaluronan recruitment to microfibrils. PMID: 23963449
altered balance of VCAN splice variants in combination with reduction in glycosaminoglycan protein modifications as pathogenic mechanisms in Wagner syndrome PMID: 22739342
Letter/Case Report: disease causing versican mutation in Wagner syndrome. PMID: 23571384
versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and subsequent vitamin D-dependent mechanisms which promote ovarian tumor progression in vitro PMID: 23424670
No association has been found between polymorphisms of rs251124 and rs3767137 loci of CSPG2 and HSPG2 genes and intracranial aneurysm in the selected population. PMID: 23568740
Wnt/beta-catenin signal transducting system regulates dermal papilla cell aggregative growth through modifying versican expression by means of acting on the versican gene upstream promoter. PMID: 23099107
these results demonstrate that versican V0 and V1 isoforms play important roles in HCC development and that versican mRNAs compete with endogenous RNAs in regulating miRNA functions. PMID: 23180826
Mesenchymal cells derived from keloid lesion (KL) cells exhibit above-normal versican production. PMID: 22951719
the glioblastoma cell line U87 stably transfected with versican isoform V2 formed tumors containing extensive vasculature PMID: 23201264
renal versican expression was significantly upregulated as compared to corresponding controls. These data show for the first time an association of renal versican isoform V0 and V1 expression with progressive renal disease. PMID: 23024773
Studies indicate that E-cadherin and versican are involved in cancer epithelial-mesenchymal transitions and metastasis. PMID: 23002209
Tissue microarray analysis revealed that epithelial expression of versican had significant relations to lymph node metastasis and pathological stages of breast cancer PMID: 22318369
E(2) treatment increases the amount of dermal HA and versican V2 via paracrine release of EGF, which may be implicated in the pro-proliferative and anti-inflammatory effects of E(2) during photoaging. PMID: 22493503
The major isoforms expressed by gastric carcinoma & gastric cell lines were V0 & V1. V1 was higher in gastric carcinoma. Abnormally expressed versican & its isoforms participate in the progress of gastric carcinoma triggered by IL-11. PMID: 22393310
RhoGDI2 suppresses lung metastasis in mouse models by reducing the expression of isoforms V1 and V3 of the proteoglycan versican, which is require for lung metastasis. High versican levels portended poor prognosis in patients with bladder cancer. PMID: 22406535
Hyaluronan and versican play a role in T-cell trafficking and function in inflamed tissues. PMID: 22155153
Versican is a key component of the provisional wound repair ECM that is expressed following injury to valvular interstitial cells. PMID: 21546273
the G1 and G3 domains of versican were upregulated and LTBP-4 was downregulated in breast cancer stroma PMID: 21505857

Hide All

Involvement in disease

Wagner vitreoretinopathy (WGVRP)

Subcellular Location

Secreted, extracellular space, extracellular matrix. Cell projection, cilium, photoreceptor outer segment. Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix.

Protein Families

Aggrecan/versican proteoglycan family

Tissue Specificity

Expressed in the retina (at protein level). Cerebral white matter and plasma. Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V1: Expressed in normal brain, gliomas, medulloblastomas,

Database Links

HGNC: 2464

OMIM: 118661

KEGG: hsa:1462

STRING: 9606.ENSP00000265077

UniGene: Hs.643801

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Code	CSB-E11884h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.