Recombinant Human Protein CYR61 protein(CYR61) (Active)

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Code CSB-AP002661HU
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Product Details

Purity >95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of >330 IU/mg.
Target Names CYR61
Uniprot No. O00622
Research Area Immunology
Alternative Names 3CH61; CCN 1; CCN family member 1; CCN1; CYR 61; Cyr61; CYR61 protein; CYR61_HUMAN; Cysteine rich angiogenic inducer 61; Cysteine rich heparin binding protein 61; Cysteine-rich angiogenic inducer 61; GIG 1; GIG1; IBP-10; IGF-binding protein 10; Igfbp 10; IGFBP-10; Igfbp10; Insulin like growth factor binding protein 10; Insulin-like growth factor-binding protein 10; Protein CYR61; Protein GIG1
Species Homo sapiens (Human)
Source E.coli
Expression Region 25-381aa
Complete Sequence TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCT PQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD
Mol. Weight 39.4 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered citrate buffer solution, 300 mM NaCl, pH 3.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Gene References into Functions
  1. CYR61 could be an adjuvant biomarker associated with the inflammatory activity of Graves' orbitopathy (GO). PMID: 28548552
  2. Serum CCN1 concentrations in Acute Heart Failure patients were significantly increased compared with those in individuals without Acute Heart Failure (237 pg/ml vs. 124.8 pg/ml, p< 0.001). PMID: 30045012
  3. Results show that mRNA and serum expression levels of Cyr61, CTGF, and VEGF in muscle tissues of patients with polymyositis (PM)/dermatomyositis (DM). These data suggest that these genes may be involved in the pathogenesis mainly by affecting the formation of blood vessels and promoting inflammatory response. PMID: 30142763
  4. the CC genotype of CYR61significantly increased the risk of acute myeloid leukemia (AML) in the dominant inheritance and the recessive inheritance. Additionally, it was shown that the rs2297141 and rs6576776 genotypes were associated with AML-M5 and AML-M2, respectively. Our findings indicated that genetic polymorphisms in the CYR61 gene may be considered potential AML risk factors in the Han Chinese population PMID: 30142822
  5. Study describes how cysteine-rich 61 (CCN1) promotes monocyte migration by upregulating monocyte chemoattractant protein-1 (CCL2) expression in osteoblasts in rheumatoid arthritis (RA) disease. CCN1 could serve as a potential target for RA treatment. PMID: 28341837
  6. MiR-365 acts as a tumor suppressor in OS, partly, by targeting CYR61 expression. PMID: 29287201
  7. CCN1 may be involved in the progression of the hepatic cirrhosishepatocellular carcinoma axis through regulating Hepatic stellate cells. PMID: 29286082
  8. study is the first to reveal that Cyr61 is elevated in ALL and promotes cell survival through the AKT/NF-kappaB pathway by up-regulating Bcl-2. PMID: 27725691
  9. PPARgamma agonists may have beneficial effects on the migration and invasion of rheumatoid arthritis-fibroblast-like synoviocytes via the downregulation of Cyr61. PMID: 27456070
  10. CCN1 stimulates CCL20 production in vitro and in vivo, and thus supports the notion that overexpressed CCN1 in hyperproliferating keratinocyte is functionally involved in the recruitment of inflammatory cells to skin lesions affected by psoriasis. PMID: 28602508
  11. Plasma Cyr61 concentration in pulmonary arterial hypertension (PAH)patients was highly increased. Cyr61 could promote pulmonary arterial smooth muscle cells proliferation via AKT pathway, indicating that Cyr61 may play a role in the pathogenesis of PAH. PMID: 28824319
  12. Cyr61 and VEGF expressions were independent prognostic indicators of overall survival and may serve as important prognostic predictors in patients with osteosarcoma. PMID: 28647210
  13. our study suggests that suppression of Cyr61 in cancer stem cell-like cells in PDAC may inhibit tumor cell metastasis after resection of the primary tumor. PMID: 27705906
  14. CYR61 promotes breast cancer lung metastasis by facilitating tumor cell extravasation and protecting from anoikis during initial seeding to the lung. PMID: 27911269
  15. Knockdown of CYR61 in gastric cancer AGS cells impairs the cancer cell migration and invasion, suggesting a driver role of CYR61 in metastasis. PMID: 27105510
  16. This study is the first to describe how CCN1 promotes VEGF expression in osteoblasts and increased endothelial progenitor cells angiogenesis in rheumatoid arthritis disease. PMID: 27465842
  17. Two interacting partners of urokinase-type plasminogen activator (uPA) receptor, the cysteine-rich angiogenic inducer 61 (Cyr61) and the Y-box-binding protein 1 (YB-1) were identified and their differential expression demonstrated in the triple-negative breast cancer (TNBC) cells as well as in tumors. PMID: 27286449
  18. collagen II-activated phosphorylated-DDR2 induces CYR61 through activation of transcription factor activator protein 1 (AP-1). The elevated CYR61, in turn, accelerates MMP1 production via ETS1 (ETS proto-oncogene 1). PMID: 27653023
  19. Cyr61 stimulated MMP-3 production in fibroblast-like synoviocytes of rheumatoid arthritis patients. PMID: 27585710
  20. Low CCN1 expression is associated with esophageal cancer. PMID: 29055676
  21. this study found that expression of Cyr61 was higher in the ectopic endometrium than in the eutopic endometrium and that Cyr61 expression in the endometrium was correlated with age, number of natural labors, blood loss, uterine volume, adenomyosis type, and concurrent endometriosis PMID: 27644084
  22. The role of CYR61 in aromatase inhibitors resistance in estrogen receptor positive breast cancer patients PMID: 27113745
  23. CYR61 is a TGF-beta-induced stromal-derived factor that regulates gemcitabine sensitivity in pancreatic ductal adenocarcinoma. PMID: 27604902
  24. These studies support previously unrecognized, cooperative interaction between the CCN1 matricellular protein and canonical TGF-beta1/SMAD3 signaling that promotes lung fibrosis. PMID: 26884454
  25. Our data indicate a potential functional impact of Cyr61 in development and the progression of cervical cancer PMID: 28476813
  26. results highlight both CYR61 and TAZ genes as potential predictive biomarkers for stratification of the risk for development of adenocarcinoma and suggest a potential mechanistic route for Barrett's esophagus neoplastic progression PMID: 27583562
  27. Reduced levels of CCN1 and CCN3, as found in early-onset preeclampsia, could contribute to a shift from invasive to proliferative extravillous trophoblasts and may explain their shallow invasion properties in this disease. PMID: 26744771
  28. Promoter activity was determined as well as CYR61 mRNA and protein expression. PMID: 28322825
  29. Our results not only reveal a novel mechanism illustrating the role of Cyr61 in epidermis pathogenesis but also suggest that therapies targeting Cyr61 may represent a novel strategy in the treatment of psoriasis vulgaris. PMID: 27856305
  30. Results provide evidence that CCN1 can stimulate the proliferation and differentiation of osteoblasts in vitro and contribute to bone remodeling in vivo in myeloma bone disease. PMID: 28035364
  31. these data suggest that the WNT/beta-catenin signaling pathway enhances pancreatic cancer development and malignancy in part via up-regulation of CYR61. PMID: 27889647
  32. CCN1 RNAi reduced the mRNA and protein expression levels of CCN1, Akt and VEGF in the HUVECs (P<0.05). Furthermore, LY294002 reduced the mRNA and protein expression levels of CCN1 in the hypoxic cells (P<0.05). Therefore, CCN1 RNAi may offer a novel therapeutic strategy, which may aid in the treatment of pathological angiogenesis via inhibition of the PI3K/AktVEGF pathway PMID: 27666419
  33. High CYR61 expression is associated with colorectal cancer. PMID: 27743169
  34. Following the treatment of B16 cells with epirubicin and IFN-a, CYR61 levels increased, cell growth was inhibited, and proliferating cell nuclear antigen expression decreased. Thus, CYR61 could become a therapeutic target for malignant melanoma patients with high CYR61 expression. PMID: 27665942
  35. The present study aimed to examine the clinical relevance of NOV along with CYR61 and CTGF in gastric cancer by analysing their transcript levels...the expression of NOV and CYR61 was increased in gastric cancer. The elevated expression of CYR61 was associated with poorer survival. NOV promoted proliferation and invasion of gastric cancer cells PMID: 27633176
  36. Taken together, our results provide novel insights into the possible protective role of 7,8-DHF by activating Cyr61 signaling and suppressing ER stress in hypoxic HK-2 cells which have potential clinical implications for the treatment of AKI. PMID: 28116298
  37. up-regulated in epithelial cells of salivary glands of primary Sjogren's syndrome patients PMID: 26630293
  38. Data show that RNA silencing of CCN family member 1 protein (CCN1) inhibits umbilical vein endothelial cell (HUVEC) proliferation under hypoxic conditions by inhibiting phosphoinositide 3-kinase (PI3K)/AKT protein signaling. PMID: 26459773
  39. Studies indicate that the CYR61 CTGF NOV matricellular proteins (CCN family of proteins) comprises the members CCN1, CCN2, CCN3, CCN4, CCN5 and CCN6 and have been identified in various types of cancer. PMID: 26498181
  40. Results suggest that Cyr61 is induced by EGF through the ERK/CREB signal pathway and that it plays a crucial role in the migration and invasion of anaplastic thyroid cancer cells. PMID: 25773758
  41. CCN1 is an injury response protein that functions not only to restrict fibrosis in the liver, but also to suppress hepatocarcinogenesis by inhibiting EGFR-dependent hepatocyte compensatory proliferation PMID: 26028023
  42. we demonstrate that NF2 negatively controls the invasiveness of Glioblastoma multiforme through YAP-dependent induction of CYR61/CCN1 and miR-296-3p. PMID: 26923924
  43. It promotes cell growth and angiogenesis in cancers through its interaction with several integrins and proved to be an independent prognostic factor for patient survival. PMID: 26201842
  44. c-Src regulates secreted proteins, including the exosomal Cyr61, which are involved in modulating the metastatic potential of triple negative breast cancer cells. PMID: 25980494
  45. Report shows evidence that CCN1 is O-fucosylated at Threonine 242 and that O-fucosylation of CCN1 regulates its secretion. PMID: 26424659
  46. Reactive oxygen species increased c-Jun, a critical member of transcription factor AP-1 complex, and increased c-Jun binding to the AP-1 site of the CCN1 promoter. PMID: 25536346
  47. Overexpression of Cyr61 may induce epithelial-mesenchymal transition and therefore leads to laryngeal squamous- cell carcinoma invasion and metastasis and poor prognosis. PMID: 25854342
  48. Cyr61 promotes CD204 expression and the migration of macrophages via MEK/ERK pathway in esophageal squamous cell carcinoma PMID: 25620088
  49. Report is first to suggest a relationship between a genetic variant p.R47W in CCN1 and atrial septal defects in humans. PMID: 25135600
  50. SYK is downstream of CYR61 and contributes to CYR61-mediated mitoxantrone resistance. The CYR61-SYK pathway represents a potential target for reducing stroma-induced chemoresistance PMID: 25974135
  51. endothelial CCN1 downregulation at least partially due to Fli1 deficiency may contribute to the development of digital ulcers in systemic sclerosis patients PMID: 25421497
  52. The expression of CYR61,that regulates fibroblast-like synoviocites proliferation and T helper 17 cell differentiation,appears to be crucial in mediating joint inflammation in Rheumatoid arthritis. PMID: 26203933
  53. CCN1 is highly elevated in cholangiocytes in human cholestatic liver. CCN1 activates NF-kappaB in differentiated cholangiocytes through integrin alphavbeta5/alphavbeta3, leading to Jag1 expression and JAG1/NOTCH1 signaling to drive cholangiocyte proliferation. PMID: 25822023
  54. CCN1 suppresses ADAMTS-4 activity and that CCN1 overexpression is directly correlated with chondrocyte cloning in osteoarthritis cartilage. The TGFbeta/CCN1 axis plays a role in chondrocyte cluster formation through inhibition of ADAMTS-4. PMID: 25709087
  55. Findings reveal that CCN1 plays a novel role in pathogen clearance; increasing macrophage infiltration and activation resulting in increased virus clearance in tumors. PMID: 24895995
  56. our findings suggest a potential role of Cyr61 in GBM growth and highlight Cyr61 as a novel target for glioblastoma therapy PMID: 25501701
  57. Cyr61 was higher in RA synovial fluid than in osteoarthritis synovial fluid. Cyr61 induced proIL-1beta production in fibroblast-like synoviocytes via the AKT-dependent NF-kappaB signaling pathway. PMID: 25728492
  58. Cyr61 promotes the Epithelial to mesenchymal transition (EMT) of osteosarcoma cells by regulating EMT markers via a signal transduction pathway. PMID: 25326651
  59. potential role of Cyr61 in Muller cell degeneration PMID: 25329584
  60. CYR61 overexpression associated with the development and poor prognosis of ovarian carcinoma. PMID: 25048722
  61. Overexpression of Cyr61 enhanced the growth of pancreatic carcinoma via nuclear exclusion of p27. PMID: 25104090
  62. results suggest a previously unknown Sox4-Cyr61 molecular network, which may control colon cancer cell proliferation and survival PMID: 25059387
  63. An association with Graves disease was shown for SNP rs3753793 in CYR61. An association with Graves ophthalmopathy was shown for several SNPs. Smoking and CYR61 rs12756618 interacted to increase the risk of Graves ophthalmopathy. PMID: 24780075
  64. integrin alpha(v) beta(3) and AMP-Activated Protein Kinases mediate CCN1-induced endothelial cell migration and tube formation independent of intracellular ATP levels alteration. PMID: 24909560
  65. Our data confirmed that Cyr61 was expressed in colorectal cancers and the expression was correlated with worse prognosis of colorectal cancers. PMID: 24606730
  66. we report that YAP and its downstream transcriptional targets CCN1 and CCN2 are markedly elevated in keratinocytes in human skin basal cell carcinoma PMID: 24485923
  67. CYR61/CCN1 overexpression in the myeloma microenvironment is associated with superior survival and reduced bone disease. PMID: 25061178
  68. CYR61 expression was significantly upregulated in ovarian carcinoma tissue compared with benign ovarian tumour tissue samples. Protein CYR61 levels were associated with lymph node metastases and Union for International Cancer Control stage. PMID: 24595148
  69. Our results here reveal a novel role of Cyr61 in the pathogenesis of rheumatoid arthritis PMID: 24517278
  70. It provides evidence that CCN1 is released from activated platelets, suggesting that platelets constitute a novel source for CCN1 release to the circulation during sepsis. PMID: 24430538
  71. we hypothesize that cCCN1 promoted the epithelial cell death and tissue loss after prolonged cigarette smoking exposure PMID: 24973403
  72. overexpressed Cyr61 plays a key role in the metastasis of osteosarcoma cells and Cyr61 is a potential target downstream of TGF-beta/p38 MAPK to regulate cell migration. PMID: 24037310
  73. Our results suggest that CCN1 has a role in the development of intraepithelial breast carcinoma. PMID: 24767859
  74. target for heparin binding, which interferes with integrin functions PMID: 24009013
  75. Overexpression of MRTF-A significantly promoted the migration of MCF-7 cells through its transactivation effects on MYL9 and CYR61 genes PMID: 24084383
  76. our results further revealed that expression of Cyr61, an extracellular matrix-associated growth factor, was negatively regulated by miR-100. PMID: 24317814
  77. Results reveal a novel mechanism whereby p53 is involved in the posttranscriptional regulation of Cyr61 expression via miRNA-22. PMID: 24449575
  78. data indicate that PC-3 cells overproduce Cyr61 protein via activation of the PI3K/Akt signaling as a part of the survival mechanisms under the conditions causing extracellular acidity and further cytotoxicity PMID: 23623839
  79. Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676
  80. Vinblastine resistance in renal cell carcinoma can be ascribed to the overexpression of P-gp, which is regulated by the activation of the PI3K/Akt pathway and triggered by upstream CCN1-integrin avb3 interaction. PMID: 23744557
  81. Cyr61/Caprin-1 co-expression was associated with worse survival. PMID: 23528710
  82. Data indicate CCN1 can induce actin reorganization, formation of lamellipodia, and cell migration/invasion through the alphaV integrins/Rac1/ERK signaling axis; these CCN1-dependent activities can be effectively suppressed by monoclonal antibody YM1B. PMID: 23618859
  83. Our results indicate that the genetic variation of rs3753793 in the CYR61 promoter may contribute to genetic predisposition to prostate cancer PMID: 23045290
  84. Cyr61 has considerable potential as a novel marker for poor prognosis in osteosarcoma. PMID: 21976359
  85. domain-specific interactions of CCN1 with alphaVbeta3 integrin contribute to human skin aging by stimulating MMP-1-mediated collagen fibril fragmentation. PMID: 23504324
  86. Data indicate the role of CYR61 and alpha(V)beta5 integrin as proteins that cooperate to mediate cancer cell migration. PMID: 22692860
  87. Blocking the PI3K signal pathway could significantly inhibit the expression of Cyr61, and may promote the apoptotic effects and inhibit cell growth of ovarian carcinoma cells. PMID: 23141184
  88. Beta-catenin-dependent expression of WISP1 and Cyr61 is critical for epithelial repair. PMID: 23316072
  89. Cyr61 is transcriptionally induced under hypoxia and is an important factor in preeclampsia. PMID: 21505992
  90. Cyr61 induces angiogenetic effect and enhances the tumorigenicity of solid tumors. PMID: 23259998
  91. Snail-dependent production of Cyr61 in epithelial mesenchymal transformation-phenotypic cells could prime and promote dynamic cell migration of the invasive tumor nest. PMID: 23196056
  92. the matricellular protein CCN1/Cyr61 is a critical regulator of Sonic Hedgehog in pancreatic carcinogenesis PMID: 23027863
  93. CCN1 is upregulated in pancreatic cancer and promotes the survival of pancreatic cancer cells. PMID: 22752926
  94. Wnt-5a and CYR61 are enriched in the extracellular matrix of liver fibrotic tissue compared with control matrix. PMID: 22694338
  95. Study demonstrates that CYR61 protein enhances the expression of VEGFC by binding with alphaVbeta3 integrin receptor on the surface of Decidual natural killer (dNK) cells through intracellular PI3K/AKT signaling pathway; may regulate the function of dNK cells and may be operative in the pathogenesis of pre-eclampsia. PMID: 22023263
  96. Given the ability of CCN1 to regulate both production and degradation of type I collagen, it is likely that elevated-CCN1 functions as an important mediator of collagen loss, which is observed in aged human skin. PMID: 22566095
  97. CCN1 plays an important role in regulating immune cell trafficking by attracting and locally immobilizing immune cells PMID: 22527715
  98. It conclude that in the murine atria, CCN1 and CCN2 proteins are expressed constitutively. In chronic heart failure, CCN proteins tend to be upregulated, which may be related to the action of angiotensin II. PMID: 22532143
  99. Cyr61 enhances the migration of oral squamous cell carcinoma cells by increasing MMP-3 expression through the alphavbeta3 or alpha6beta1 integrin receptor, FAK, MEK, ERK, and NF-kappaB signal transduction pathway PMID: 22253074
  100. a novel, VEGF-independent, clinically relevant, pro-angiogenic factor, CYR61, that is a transcriptional target of Hh-GLI signaling PMID: 22056874

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Subcellular Location Secreted
Protein Families CCN family
Database Links

HGNC: 2654

OMIM: 602369

KEGG: hsa:3491

STRING: 9606.ENSP00000398736

UniGene: Hs.8867

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