Recombinant Human Interleukin-1 receptor antagonist protein(IL1RN) (Active)

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Code CSB-AP001681HU
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Product Details

Purity >96% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α- dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of >2.5x104 IU/mg in the presence of 50 pg/ml rHuIL-1α.
Target Names IL1RN
Uniprot No. P18510
Research Area Immunology
Alternative Names DIRA; F630041P17Rik; ICIL 1RA; ICIL-1RA; ICIL1RA; IL-1ra; IL-1ra3; IL-1RN; IL1 inhibitor; IL1F3; IL1RA; IL1RA_HUMAN; IL1RN (IL1F3); IL1RN; Interleukin 1 receptor antagonist; Interleukin-1 receptor antagonist protein; Intracellular IL 1 receptor antagonist type II; Intracellular interleukin 1 receptor antagonist (icIL 1ra); IRAP; MGC10430; MVCD4; Type II interleukin 1 receptor antagonist
Species Homo sapiens (Human)
Source E.coli
Expression Region 26-177aa
Complete Sequence RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE
Mol. Weight 17.1 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Gene References into Functions
  1. PDE4B-deficient mice displayed an increased circulating IL-1Ra, suggesting a protective role of PDE4B inactivation in vivo. PMID: 28383060
  2. Interleukin 1 receptor antagonist*A2 might be a risk factor for progressive vitiligo. PMID: 29620037
  3. IL1-Ra is an independent predictor for adverse outcomes in patients with documented coronary artery disease. PMID: 29395365
  4. Carrying allele IL1RN*2 had a strong association with idiopathic recurrent spontaneous abortion (iRSA) in Mexican women; this polymorphism codifies for a low-function protein, which may allow for increased activity of IL-1 pro-inflammatory axis in iRSA. PMID: 29718011
  5. there was no sufficient evidence to support the association between IL-1RN 86-bp VNTR polymorphism and ischemic stroke (Meta-Analysis) PMID: 30075593
  6. Serum interleukin 1 receptor antagonist (IL1RA) and granzyme B (GZMB) levels were markedly increased in Crohn's disease (CD) patients, suggesting that these markers can serve as biomarkers to identify gut inflammation. PMID: 28972805
  7. this study reflects the importance of the IL1RN*2/2 genotype and its possible association in the pathogenesis of RA by regulation in the IL-1Ra production. PMID: 29226727
  8. Data show that the frequency of the IL-1 receptor antagonist (IL-1RN) 'T' allele of rs928940 was significantly lower in BC cases than in controls. PMID: 29047186
  9. TNF-alpha -857 C/C variant might represent protective effect against recurrent pregnancy loss (RPL) and the -857 C/T variant might be a genetic risk factor for the occurrence of RPL. Invariant differences in the prevalence of -511 C/T and -31 C/T polymorphisms and IL-1RN VNTR between RPL patients. PMID: 29949333
  10. IL-1ra concentrations were greater (p = 0.0018) in bone marrow aspirate samples (13,432 pg/mL) than in platelet-rich plasma (588 pg/mL). PMID: 26831858
  11. Studied role of IL-1Ra VNTR variant in susceptibility of temporomandibular joint disorders (TMD), and found the variant is significantly associated with TMD. PMID: 28612927
  12. Studied interleukin-1 receptor antagonist (IL-1Ra) and angiotensin-converting enzyme (ACE) I/D polymorphisms with regards to the susceptibility of patients to carpal tunnel syndrome. PMID: 28370589
  13. we did not observe an association between IL-1Ra 86 bp VNTR polymorphism in intron 2 and RPL patients (p > 0.05). Conclusion IL-1Ra VNTR polymorphism may not be a genetic factor for RPL PMID: 28593919
  14. The presence of the rs2234663 A2/A2 genotype in rheumatoid arthritis is associated with increased disease activity. PMID: 28342152
  15. The results suggest that although the 1068 G>A polymorphism of the P2RX7 gene is associated with an increased beta-cell function and IL-1Ra release in type 2 diabetes patients, the glycemic control is not significantly affected by the presence of this SNP. PMID: 29425823
  16. Studies used adult stem cells to engineer anatomically shaped, functional cartilage constructs capable of tunable and inducible expression of antiinflammatory molecules, specifically IL-1 receptor antagonist (IL-1Ra). PMID: 27432980
  17. No significant associations with RHD were found for the IL1RN rs447713 and CTLA4 rs3087243 SNPs. PMID: 27400406
  18. Reconstitution of ST2 (IL-1R4) specific for IL-33 activity; no suppression by IL-1Ra though a common chain IL-1R3 (IL-1RAcP) shared with IL-1 PMID: 27031441
  19. The polymorphic expression of IL-1RN (rs419598) gene may be associated with a reduced susceptibility to GAgP and GCP in populations of European descent. PMID: 29023524
  20. associations between the genetic variants and the LPS-induced IL-6, IL-8, IL-10, IL-1ra and TNF-alpha cytokine levels were not significant in the meta-analysis. This present study does not support a strong genetic effect of LPS-stimulated cytokine production; however, the potential for type II errors should be considered PMID: 23823136
  21. IL1RA intron 2 VNTR seems to be a genetic marker for overall adiposity status in Malaysian subjects. PMID: 28293435
  22. Increased IL-1Ra levels in women with polycystic ovary syndrome were largely explained by increasing adiposity. However, serum IL-1Ra concentrations predicted 2-h glucose levels independently of BMI suggesting that increased IL-1Ra may be associated with disturbed glucose metabolism. PMID: 27061312
  23. demonstrated that IL-1RN VNTR polymorphism might increase the risk of H. pylori infection, especially in Asians PMID: 28384207
  24. VNTR polymorphism associated with osteomyelitis development PMID: 28682145
  25. Polymorphisms of Il1rn were not significantly associated with bipolar I disorder in Iranian patients. PMID: 28129679
  26. Meta-analysis: Serum IL-1RA levels were positively associated with risk of cardiovascular disease. This association may at least partially reflect a response to triggers inducing subclinical inflammation, oxidative stress, and endothelial activation. PMID: 28428221
  27. Our work highlights the convenience and efficiency of this novel pH-sensitive fluorescent probe and reveals the new biological activity of staurosporine as an agonist for GLUT4 translocation and as an effective insulin additive analogue. PMID: 27769857
  28. African American women are at increased risk for early birth, particularly via the inflammatory pathway. Variants of the IL1RN gene, which encode the interleukin-1 receptor antagonist (IL-1Ra) protein, are implicated in early birth PMID: 28252571
  29. This study reveled that the association of IL1RN haplotype containing RN2 with FIRES, and showed a possible association of IL1RN rs4251981 G > A. PMID: 27538648
  30. We found that the mean mRNA levels of the proinflammatory cytokines IL-1beta, IL-6, TNF-alpha, their receptors, TNFR1, TNFR2, IL-1R1 and the antagonist IL-1RA were significantly increased in the lymphocytes of MDD patients compared with NC [normal control subjects].. PMID: 27138824
  31. C/T genotype of IL-1Ra +2018 prognosticates more aggressive disease in RA PMID: 27105431
  32. this study shows that IL-1Ra gene variants are associated with susceptibility to juvenile idiopathic arthritis in Iranian population PMID: 27717726
  33. The A2 allele of the interleukin 1 receptor antagonist VNTR polymorphism could be a protective factor for pre-eclampsia susceptibility. PMID: 26555681
  34. A2 allele and the combined IL-4 (low) -IL-1Ra (high) genotype might be genetic markers of susceptibility to frailty in Mexican elderly. PMID: 26646252
  35. This study demonstrated that thr frequency of the IL-1Ra/C allele at position Mspa-I 11100 was decreased significantly and the IL-1Ra/T frequency was significantly increased in patients with Febrile Seizures. PMID: 26500244
  36. Data indicate that levels of interleukin 1 receptor antagonist protein (IL-1-Ra) remain elevated for at least one year after storage at -80 degrees C. PMID: 26994310
  37. study showed that, IL-1RN allele 2, periodontal disease characterized with clinical attachment loss, previous preterm/low birth weight outcome (PLBW) and age could be important risk factors for PLBW PMID: 26445016
  38. Significant associations were found between Crohn's disease (CD) and minor NOD2 variants, as well as TLR4 299Gly, TNF-alpha G-308A, IL-6 G-174C and IL-1RN VNTR A2 variants, while ulcerative colitis (UC) was associated only with IL-1RN VNTR A2 variants. PMID: 26316104
  39. Suggest that IL-1RN rs315952 polymorphism may not be associated with susceptibility to Tourette syndrome in Chinese Han population. PMID: 26097611
  40. Serum IL1RA and IL2RA are predictors of event free survival in T-cell lymphoma. PMID: 26487586
  41. IL-1Ra can be a useful tool in the diagnosis of hepatic inflammation. PMID: 26612588
  42. This study shows a significant association between IL-1 RA allele distribution and febrile convulsions PMID: 26813462
  43. IL-1 genotypes do not seem to be good predictors of peri-implantitis in the great majority of smoking patients PMID: 26449434
  44. the lower expression of IL10 and the higher expression of IL1RA in Mo exposed to arthritic than to non-arthritic SF suggest that arthritic SF is mainly reducing the inflammatory responses in Mo. PMID: 26521731
  45. Results indicate there is a lack of association between the IL1RN VNTR polymorphisms and tuberculosis risk. [Meta-Analysis] PMID: 26330006
  46. The IL-4 rs79071878 polymorphism, was associated whereas the IL-1Ra rs2234663 polymorphism was not associated with Familial Mediterranean Fever risk in the Turkish population. PMID: 26861613
  47. While both contributing to pathogen clearance, NLRP3 more than NLRC4 contributes to deleterious inflammatory responses in cystic fibrosis and correlates with defective NLRC4-dependent IL-1Ra production. PMID: 26972847
  48. These data suggest that M. leprae upregulates IL-1Ra by a TOLLIP-dependent mechanism; inhibition of TOLLIP may decrease an individual's susceptibility to leprosy and offer a novel therapeutic target for IL-1-dependent diseases. PMID: 26610735
  49. This study emphasizes a positive association between IL1-Ra (VNTR) polymorphism and DM among Saudi children. This may suggest that (A2) allele may play important role in disease susceptibility. PMID: 26502861
  50. IL1-RN VNTR A1A3 genotype is associated with higher risk of RA. Among the cases, males who carry this genotype were more exposed to RA and had less erosive forms. PMID: 26003199
  51. IL-1Ra is associated with MMP-13 expression and has a novel function in such regulation without interference of the IL-1 signaling cascade. PMID: 26474296
  52. Thus, pathogenic inflammasome activity during Candida infection is negatively regulated by the IL-22/NLRC4/IL-1Ra axis. PMID: 26269955
  53. IL-1RN VNTR polymorphisms were associated with good stroke outcomes. PMID: 25223242
  54. In stroke patients rs4251961 single nucleotide polymorphism in IL1RN was associated with self-reported fatigue. PMID: 25542762
  55. For each IL1RN minor allele inherited, serum concentrations of IL-1Ra increased by 0.22 SD, as did cardiometabolic risk. PMID: 25726324
  56. IL-1RN and IL-4 gene polymorphisms are potential genetic markers of susceptibility to bladder cancer. PMID: 24850227
  57. These data suggest that IL1RN may exhibit opposing characteristics in oral malignancies depending on the stage of cancer development, suppressing early carcinogenic events, yet promoting tumor development in some lesion sites. PMID: 25738940
  58. maternal and fetal carriage of IL1RN*2 increased the risk of preterm birth only in studies including preterm premature rupture of membranes [meta-analysis] PMID: 24588341
  59. The data of the present study clearly suggests that IL1RN variable numbers of tandem repeat polymorphism is a genetic risk factor for pregnancy loss in the study population. [meta-analysis] PMID: 24952603
  60. associations of IL1 receptor antagonist and estrogen receptor gene polymorphisms with systemic lupus erythematosus susceptibility PMID: 25286391
  61. IL8, IL-1Ra, MCP-1, and MIP-1beta are significantly lower in patients with type 1 diabetes than controls. PMID: 26158606
  62. Results showed the salivary expression level of IL-4, 10, 13 and IL-1RA were raised in oral squamous cell carcinoma patients and IL-1RA were correlated with poorly differentiated tumors. PMID: 26046681
  63. Our data suggest that the variable number of tandem repeats IL1RN polymorphism may not be associated with the risk of pulmonary tuberculosis in a sample of Iranian population. PMID: 25530139
  64. Data indicate that Candida albicans beta-Glucan induces high IL-1 receptor antagonist (IL-1Ra) levels, independently from Dectin-1/complement receptor 3 (CR3). PMID: 25461401
  65. IL1 receptor antagonist, variable number tandem repeat may be associated with external apical root resorption PMID: 22882407
  66. The IL1RN 86-bp VNTR polymorphism is probably associated with sepsis risk but not with sepsis-related mortality. PMID: 25500257
  67. Of the three cytokines measured, only IL-1ra was statistically significantly elevated in cancer patients when compared with control participants. PMID: 26101331
  68. IL-1 SNPs seem to be associated with inflammatory bowel disease in Iranian patients and could affect the disease severity as well. PMID: 25466956
  69. these results identify sIL-1Ra as a novel mutant p53 target gene, whose suppression might be required to generate a chronic pro-inflammatory tumor microenvironment through which mutant p53 promotes tumor malignancy. PMID: 24998848
  70. IL-6 and IL-1RA polymorphisms as well as the overexpression of IL-6 might have significant implications in the pathogenesis of ID. PMID: 25124963
  71. IL1RN gene polymorphism is associated with gastritis. PMID: 25292060
  72. Polymorphisms 1/1 (IL-1RA over-producer) was infrequent and 1/3 and 2/3 (under-producers) frequent among irritable bowel syndrome. PMID: 25073651
  73. Elevated IL-2R, IL-1RA, and CXCL9 are associated with shorter event-free survival in newly diagnosed follicular lymphoma, treated with chemoimmunotherapy. PMID: 25422100
  74. IL1RN polymorphism was associated with increased risk of cervical cancer. PMID: 24905619
  75. IL1RN may influence the risk of infection after stroke. PMID: 24233813
  76. No significant association was observed between the serum levels IL-5 and IL-1ra and the rate of tobacco smoking in AEP patients. PMID: 24706315
  77. Polymorphisms for the genes ACE(I/D), CCR-5, IL-1RA, MBL-2, TLR-4, and IL-4 were typed by polymerase chain reaction (PCR) and/or PCR-restriction fragment length polymerase PMID: 24819208
  78. Our results do not confirm an association between any IL1RN genotype and ischemic stroke in Caucasian patients. PMID: 24680088
  79. SFAs, but not unsaturated fatty acids (UFAs), increased interleukin (IL)-1beta secretion and decreased IL-1 receptor antagonist (IL-1Ra) secretion, resulting in an increase in the IL-1beta/IL-1Ra secretion ratio PMID: 25201247
  80. rs4251961 at the IL1RN locus is associated with increased circulating levels of IL-1 receptor antagonist, a protective factor in development of insulin resistance. PMID: 24969107
  81. In Graves disease orbital fibroblasts, relatively high basal PTEN levels prevent secreted IL-1RA expression or release. PMID: 24840811
  82. It may be that genetically determined IL1RA levels influence survival from septic shock. PMID: 25089931
  83. We demonstrate that Aspergillus fumigatus galactosaminogalactan inhibits cytokines production by inducing Interleukin-1 receptor antagonist PMID: 24603878
  84. this preliminary study documents that serum IL-1RA and IL-18 levels are increased in some children with acute urticaria PMID: 24490166
  85. Elevated levels of hippocampal IL1RN are causally related to some Alzheimer's disease-associated memory disturbances. PMID: 23954849
  86. the IL1RN *2 polymorphism is associated with susceptibility to SLE in Europeans and Asians. PMID: 24055697
  87. These data characterize multiple robust genetic signals that influence IL-18 and IL-1ra cytokine production. PMID: 24182552
  88. Meta-analysis results revealed that genetic variants in IL-1 and IL-1RN genes were associated with an increased risk of IgA nephropathy. similar associations were not observed in IL-6, IL-10, and IL-22R genes. [Meta-analysis] PMID: 24592969
  89. IL-1RA is associated with all other groups of mediators and is the only mediator which correlates (positively) with residual beta cell function PMID: 23991111
  90. TSH induces the expression of IL-1RA in fibrocytes and Graves'disease orbital fibroblasts. PMID: 24446657
  91. both serum concentration of pro-hepcidin and IL-1RN genotypes frequency reflect the disease activity, regardless of the anemia states in Rheumatoid arthritis (RA )patients, thus they may be another potential markers for disease activity of RA. PMID: 24014495
  92. Recipient IL-1RN polymorphisms were not associated with the presence of pneumonia in kidney transplantation. PMID: 23953530
  93. These data indicated that IL-1Ra, IL-6, IL-8, and TNF-alpha were associated with increased risk of Osteosarcoma, in which IL-8 and TNF-alpha may be further correlated with the progression of this disease PMID: 23999825
  94. IL-1RN VNTR gene polymorphism may be involved in the pathogenesis of urinary stone formation, contributing to genetic susceptibility for urolithiasis PMID: 23363559
  95. no association between IL-1RN variable number of tandem repeats and duodenal ulcer with or without Helicobacter pylori infection in overall population [meta-analysis] PMID: 23800434
  96. The study indicates IL-1RN*A2 homozygosity as a significant risk marker for nasopharyngeal carcinoma in a Portuguese population. PMID: 23562526
  97. findings show the IL-1RN VNTR polymorphism may not be an independent major contributor to ischemic stroke in the Chinese Uyghur population PMID: 23743874
  98. A significantly greater likelihood of radiological progression of knee osteoarthritis was associated with a commonly occurring IL1RN haplotype that could be tagged by three IL1RN single nucleotide polymorphisms. PMID: 23602982
  99. A pilot case-control study was conducted to evaluate the role of IL-1 receptor antagonist (IL-1RN) VNTR penta-allelic polymorphism in asthma. PMID: 23602199
  100. the "innate immune set point" defined by the early levels of sCD14 and IL-1RA might be powerful early surrogate markers for disease progression and should be considered for use in clinical practice. PMID: 23818854

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Involvement in disease Microvascular complications of diabetes 4 (MVCD4); Interleukin 1 receptor antagonist deficiency (DIRA)
Subcellular Location Isoform 1: Secreted, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, SUBCELLULAR LOCATION: Isoform 4: Cytoplasm
Tissue Specificity The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Database Links

HGNC: 6000

OMIM: 147679

KEGG: hsa:3557

STRING: 9606.ENSP00000259206

UniGene: Hs.81134

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