Product Name:

AGO2 Antibody

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Code : CSB-PA891731LA01HU
Size : US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot NO. : Q9UKV8
Storage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image :
  • Western blot
    All lanes: Protein argonaute-2 antibody at 2ug/ml
    Lane 1: A549 whole cell lysate
    Lane 2: Jurkats whole cell lysate
    Lane 3: MCF-7 whole cell lysate
    Lane 4: HepG2 whole cell lysate
    Lane 5: Raw264.7 whole cell lysate
    Lane 6: K562 whole cell lysate
    Lane 7: Mouse liver tissue
    Lane 8: Mouse kidney tissue
    Secondary
    Goat polyclonal to Rabbit IgG at 1/10000 dilution
    Predicted band size: 98, 94 kDa
    Observed band size: 90 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA891731LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA891731LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA891731LA01HU at dilution of 1:100

  • Chromatin Immunoprecipitation Hela(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP891731HU) against the SLC1A5 promoter.

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA891731LA01HU at dilution of 1:100

Immunogen : Recombinant human Protein argonaute-2 protein (517-818AA)
Raised in : Rabbit
Species Reactivity : Human, Mouse
Tested Applications : ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
Relevance : Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Form : Liquid
Conjugate : Non-conjugated
Storage Buffer : Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification method : >95%, Protein G purified
Isotype : IgG
Clonality : Polyclonal
Alias : Protein argonaute-2 (Argonaute2) (hAgo2) (EC 3.1.26.n2) (Argonaute RISC catalytic component 2) (Eukaryotic translation initiation factor 2C 2) (eIF-2C 2) (eIF2C 2) (PAZ Piwi domain protein) (PPD) (Protein slicer), AGO2, EIF2C2
Species : Homo sapiens (Human)
Research Area : Epigenetics and Nuclear Signaling
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