AGO2 Antibody

Code CSB-PA891731LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA891731LA01HU at dilution of 1:100

  • IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Western blot
    All lanes: Protein argonaute-2 antibody at 2μg/ml
    Lane 1: A549 whole cell lysate
    Lane 2: Jurkats whole cell lysate
    Lane 3: MCF-7 whole cell lysate
    Lane 4: HepG2 whole cell lysate
    Lane 5: Raw264.7 whole cell lysate
    Lane 6: K562 whole cell lysate
    Lane 7: Mouse liver tissue
    Lane 8: Mouse kidney tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 98, 94 kDa
    Observed band size: 94 kDa

  • Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP891731HU) against the SLC1A5 promoter.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AGO2 Polyclonal antibody
Uniprot No. Q9UKV8
Target Names AGO2
Alternative Names Ago 2 antibody; AGO2_HUMAN antibody; Argonaute 2 antibody; argonaute 2; RISC catalytic component antibody; Argonaute RISC catalytic component 2 antibody; Argonaute2 antibody; CTA-204B4.6 antibody; dAgo2 antibody; eIF 2C 2 antibody; eIF-2C 2 antibody; eIF2C 2 antibody; Eif2c2 antibody; Eukaryotic translation initiation factor 2C 2 antibody; Eukaryotic translation initiation factor 2C subunit 2 antibody; hAgo2 antibody; MGC3183 antibody; PAZ Piwi domain protein antibody; PPD antibody; Protein argonaute-2 antibody; Protein slicer antibody; Q10 antibody; Slicer protein antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Protein argonaute-2 protein (517-818AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:100-1:1500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Gene References into Functions
  1. Here we show that biotin-labelled miR-34a can be loaded to AGO2, and AGO2 immunoprecipitation can pulldown biotinylated miR-34a (Bio-miR pulldown). RNA-sequencing (RNA-seq) of the Bio-miR pulldown RNAs efficiently identified miR-34a mRNA targets, which could be verified with luciferase assays PMID: 29028450
  2. extracellular vesicles are efficiently internalized by endothelial cells, where the miRNA-Argonaute 2 complexes modulate target gene expression and barrier properties. PMID: 27721445
  3. CASC7 expression was significantly decreased in colorectal cancer (CRC) tissues and CRC cell lines; CASC7 overexpression could inhibit cell viability, migration and invasion, and promote apoptosis in CRC cells PMID: 28954383
  4. A dual role of the association between AGO2 and ERbeta in luminal-like breast cancer cells in the nucleus and the cytoplasm, for the regulation of gene expression at both the transcriptional and post-transcriptional level. PMID: 29017520
  5. Phosphorylation of AGO2 at Ser 387 by Akt3 induces LIMD1 binding, which in turn enables AGO2 to interact with TNRC6A and downstream effector DDX6. PMID: 28683311
  6. AGO2-mediated cleavage of targets is more common than previously thought. This may explain the vital role of endonuclease activity in controlling miRNA-mediated gene regulation. PMID: 29031931
  7. Data show that neuropilin 1 (NRP1) binds extracellular AGO2 (carrying miRNA or not), and internalizes AGO2/miRNA complexes. PMID: 27486976
  8. We found a much larger number of microparticles (MPs) results demonstrate that normal RBCs display an innate ability to resist infection by P. falciparum parasite by releasing Ago2-miRNA complexes via microparticles (MPs)into infected RBCs; data suggest that, through release of MPs, mature RBCs present an innate resistance to malaria infection PMID: 28831191
  9. we describe these two methodologies that we recently used to select a specific compound able to interfere with the AGO2 functional activity and able to improve the retinoic acid-dependent myeloid differentiation of leukemic cells. PMID: 27924485
  10. Here, we describe the use of SPR techniques to study the interaction between Argonaute 2 and small molecular compounds selected by means of high-throughput docking screening. PMID: 27924486
  11. Since miRNAs' functions are executed exclusively by the Argonaute 2 protein, we therefore describe a protocol for the design of a novel miRNA inhibitor class: antagonists of the miRNA-Argonaute 2 protein complex, so-called anti-miR-AGOs, that not only block the crucial binding site of the target miRNA but also bind to the protein's active site. PMID: 27924487
  12. Using our recent work on human AGO2 as an example, we explain the rationale and the workflow of our method in details. This combined approach holds great promise to complement experiments in unraveling the mechanisms of molecular recognition between large, flexible, and complex biomolecules. PMID: 27924488
  13. Here, we present techniques to kinetically characterize recombinant Argonaute 2-mediated guide and target binding as well as target RNA slicing. We focus on fluorescence-based steady-state and in particular pre-steady-state techniques to unravel mechanistic details. Furthermore, we describe a cleavage assay to analyze Argonaute 2-mediated slicing using radioactively labeled target strands. PMID: 27924489
  14. This study employed molecular dynamics simulation to investigate the dynamic properties of human Ago2-RNA-duplex system and Ago2-free system to provide further understanding of the molecular mechanism of Ago2-RNA recognition. PMID: 28388001
  15. The adenovirus major late promoter produces a 31-nucleotide transcriptional start site small RNA (MLP-TSS-sRNA) that retains the 7-methylguanosine (m7G)-cap and is incorporated onto Ago2-containing RNA-induced silencing complexes (RISC) in human adenovirus-37 infected cells. PMID: 28839112
  16. Depletion of AUF1 abolishes the global interaction of miRNAs and AGO2. Single-molecule analysis revealed that AUF1 slowed down assembly of AGO2-let-7b-mRNA complex unexpectedly. AUF1 is a decay-promoting factor influencing multiple steps in AGO2-miRNA-mediated mRNA decay. PMID: 28334781
  17. This lack of 21-3U HCV host factor activity correlated with reduced recruitment of Ago2 to the HCV S1 site. Additional experiments demonstrated strong preference for guanosine at nt 22 of miR-122. Our findings reveal the importance of non-templated 3 miR-122 modifications to its HCV host factor activity, and identify unexpected differences in miRNA requirements for host gene suppression versus RNA virus replication. PMID: 28082397
  18. Low AGO2 expression is associated with melanoma. PMID: 27518285
  19. Altogether, these data suggest that the phosphorylation state of the serine/threonine cluster is important for Argonaute-mRNA interactions. PMID: 28645918
  20. data indicates that the aberrant expression of miR-15b contributes to abnormal placentation by targeting argonaute 2 messenger RNA PMID: 27208409
  21. findings support a model in which an AGO2 phosphorylation cycle stimulated by target engagement regulates miRNA:target interactions to maintain the global efficiency of miRNA-mediated silencing PMID: 28114302
  22. deciphering Ago2:RNA interactions using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across 2200 target transcripts PMID: 27418678
  23. AGO2 immunoprecipitation revealed LATS1 as a novel proapoptotic target of miR-21 in T cells. PMID: 28075055
  24. 1174 regions within the 45S rRNA transcript that have the ability to form a perfect duplex with position 2-6 (seed sequence) of each microRNA expressed in HEK293T cells. Of these potential AGO2 binding sites, 479 occurred within experimentally verified AGO2-rRNA cross-linking sites. The ability of AGO2 to cross-link to rRNA was almost completely lost in a DICER knock-out cell line. PMID: 27288410
  25. this study analysed binding of miR-122/ Argonaute 2 complexes to two conserved binding sites in the 5' UTR of hepatitis C virus RNA. PMID: 28008821
  26. overactivity of KRAS due to mutation inhibits localization of Ago2 to multivesicular endosomes (MVEs) and decreases Ago2 secretion in exosomes. PMID: 27117408
  27. TP53 regulates miRNA association with AGO2 to remodel the miRNA-mRNA interaction network PMID: 26701625
  28. These results support the notion that the cereblon binding partner AGO2 plays an important role in regulating MM cell growth and survival and AGO2 could be considered as a novel drug target for overcoming IMiD resistance in MM cells. PMID: 27142104
  29. KRAS engages AGO2 to enhance cellular neoplastic transformation. PMID: 26854235
  30. Authors found that target binding of core-RISC starts at the seed region of the guide RNA. After target binding, four distinct reactions followed: target cleavage, transient binding, stable binding, and Argonaute 2 unloading. PMID: 26592935
  31. AGO2 suppression by miR-132 affects the steady state levels of miR-221 and miR-146a, two miRNAs involved in angiogenesis and inflammation, respectively. PMID: 26475020
  32. DIS3L2 interacts with Ago2 and governs target RNA-directed miRNA degradation. PMID: 26809675
  33. MiR-138 suppresses hTERT expression in an AGO2-dependent manner. PMID: 26507454
  34. Knockdown of Ago2 in Huh7 cells and SMMC-7721 cells substantially decreased VEGF expression, whereas the restoration of AGO2 reversed both VEGF expression and secretion. PMID: 25937637
  35. genetic polymorphism in AGO2 may be a risk factor for the advanced lymph node metastasis of NPC in Chinese populations, and AGO2 acts as an oncogene in the development of NPC. PMID: 26545861
  36. Human Argonaute2 (Ago2) possesses a solvated surface pocket that specifically binds adenine nucleobases in the 1 position (t1) of target RNAs. PMID: 26359634
  37. the subcellular distribution of Ago2 occurs in a cell type- and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression PMID: 26699195
  38. HuR and Ago2 Bind the Internal Ribosome Entry Site of Enterovirus 71 and Promote Virus Translation and Replication PMID: 26451954
  39. GRSF1 participated in the regulation of AGO2 by miR-346, and the middle sequence of miR-346 was vital for the synergy effect of miR-346 and GRSF1. PMID: 26518874
  40. Human AGO2 interacts only with sRNAs (20-30 nt in length), and not with DNA fragments. PMID: 25970378
  41. SERPINE1 mRNA dissociates from the translational repressor proteins Ago2 and TIA-1 upon platelet activation PMID: 25673011
  42. Both argonaute 2 mRNA and protein were upregulated in high-grade when compared to low-grade tumor tissues. Multivariate analysis revealed that argonaute 2 protein expression was independently associated with the overall survival. PMID: 24935600
  43. Targeting of Ago2 to P-bodies and stress granules is separable from its role in RNAi and likely requires dynamic phosphorylation of serine 798. PMID: 26443379
  44. mRNA Targeting to Endoplasmic Reticulum Precedes Ago Protein Interaction and MicroRNA (miRNA)-mediated Translation Repression PMID: 26304123
  45. Ago2 can be SUMOylated in cells by both SUMO1 and SUMO2. SUMOylation occurs primarily at K402, and mutation of the SUMO consensus site surrounding this lysine reduces Ago2-mediated siRNA-induced silencing in a luciferase-based reporter assay. PMID: 26188511
  46. Ago2 overexpression increased the level of miR-16 in cell-secreted MVs, suggesting that Ago2 may facilitate the packaging of secreted miRNAs into MVs. PMID: 25072345
  47. It was demonstrated by deep sequencing that AGO2-processed AgoshRNAs produce RNA effector molecules with more discrete ends than the products of the regular shRNA design. PMID: 25826416
  48. Sumoylation of Ago2 at Lys402 negatively regulates its stability. PMID: 25036361
  49. our results indicate that IRF7 promotes glioma cell invasion and both chemoresistance and radioresistance through AGO2 inhibition PMID: 25680411
  50. a novel mechanism by which AUF1 binding and transfer of microRNA let-7 to AGO2 facilitates let-7-elicited gene silencing. PMID: 26253535
  51. Study used single-molecule Fluorescence Resonance Energy Transfer to directly visualize how human Argonaute-2 searches for and identifies target sites in RNAs complementary to its miRNA guide; study shows that miRNA targeting by human Ago2 is a highly dynamic process that has been optimized for fast and efficient target recognition. PMID: 26140593
  52. AGO2 binding to tRNA genes has a novel and important regulatory role in human cells. PMID: 25918241
  53. A dual function for MOV10 in regulating translation: it facilitates microRNA-mediated translation of some RNAs, but it also increases expression of other RNAs by preventing AGO2 function. PMID: 25464849
  54. findings indicate that in human cells AGO2 binds SWI/SNF and a novel class of sRNAs to establish nucleosome occupancy on target TSSs PMID: 25605800
  55. High Ago2 expression levels were associated with prostate cancer. PMID: 25135428
  56. Low AGO2 expression is associated with invasive breast carcinoma. PMID: 24574065
  57. Results show that FMRP regulates miR196a-mediated repression of HOXB8 via interaction with the AGO2 MID domain. PMID: 24727796
  58. Data show that downregulation of AGO2 represses invasion, migration and epithelial-mesenchymal transition in prostate cancer cells. PMID: 24805183
  59. Guided by DNA double-strand breaks induced small RNAs Ago2 can promote Rad51 recruitment and/or retention at DNA double-strand breaks to facilitate repair by homologous recombination. PMID: 24662483
  60. nature of the interactions of the EIF2C2/Ago2 PAZ domain PMID: 24788663
  61. RPL5 binds to the TRBP2 and Ago2 subunits of the RISC and mediates the binding to c-Myc 3'UTR. PMID: 24141778
  62. Phosphorylation of AGO2 at Tyr 393 inhibited loading with microRNAs (miRNAs) and thus miRNA-mediated gene silencing, which counteracted the function of H-RAS(V12)-induced oncogenic miRNAs. PMID: 25175024
  63. this study determined crystal structures of human Argonaute-2 (Ago2) bound to a defined guide RNA with and without target RNAs representing miRNA recognition sites. PMID: 25359968
  64. Data show that argonaute 2 (AGO2) can drive neovessel formation in vitro and in vivo by dysregulating the expression of some angiogenic miRNAs. PMID: 24886719
  65. Data show that trimming of Ago2-cleaved precursor miRNAs is not essential for target silencing, indicating that RISC is functional with miRNAs longer than the mature length. PMID: 24209750
  66. This study identifies the targeting of Ago2 by miR-184 as an essential component of the compensatory response to regulate proliferation according to insulin sensitivity. PMID: 24361012
  67. Findings highlight Ago2 as a new factor in myeloid cell fate determination in acute myeloid leukemia cells. PMID: 24263100
  68. Ago2 was different between HER-2 positive and HER-2 negative groups. PMID: 23775134
  69. HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference PMID: 24023945
  70. On the basis of the human Ago2 PAZ models, the indicators of stronger binding as the total binding energy and the free energy were associated with better RNAi efficacy PMID: 23877830
  71. MiR-184 regulates the expression of AGO2. PMID: 23696368
  72. Highly complementary target RNAs destabilize the complex and significantly accelerate release of the guide RNA from Ago2. PMID: 23664376
  73. Data suggest that short RNA (additional RNA), and miR-122 activates the endonuclease activity of Ago2, resulting in cleavage of the Hepatitis C virus (HCV) 5' UTR. PMID: 23512276
  74. a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response. PMID: 23824327
  75. Introducing AAs 1-64 & 137-160 of Ago2 into Ago3 are sufficient to turn Ago3 into a slicer enzyme. PMID: 23665583
  76. 2 new N-terminal Ago2 motifs (AAs 44-48 & 134-166) are key for slicing activity. The data support a dual role of the Ago2 N domain in RISC activation and mRNA cleavage. PMID: 23748378
  77. interactions between the 5' nucleotide binding domain of AGO2 and chemically modified nucleotide analogues are investigated PMID: 23289589
  78. Abnormal expression of Argonaute2 (EIF2C2) may be correlated with colon tumorigenesis. PMID: 23411422
  79. Argonaute2 promotes tumor metastasis by way of up-regulating focal adhesion kinase expression in hepatocellular carcinoma. PMID: 23258480
  80. Overexpression of human Argonaute2 inhibits cell and tumor growth. PMID: 23201202
  81. EGFR, which is the product of a well-characterized oncogene in human cancers, suppresses the maturation of specific tumour-suppressor-like miRNAs in response to hypoxic stress through phosphorylation of argonaute 2 (AGO2) at Tyr 393 PMID: 23636329
  82. findings show that Argonaute-2 (Ago2) is required for miR-122-dependent replication of Hepatitis C Virus, but dispensable for miR-122-independent replication, confirming Ago2's role in mediating the activity of miR-122 PMID: 23245472
  83. The majority of the secreted miRNAs were located in cell-secreted microvesicles, and these microvesicles-encapsulated miRNAs were bound to AGO2 complexes at various degrees. PMID: 23077538
  84. These results suggest that AGO2 and HBV-specific miRNAs might play a role in the HBV life cycle. PMID: 23091627
  85. Data show that the miRNA-processing enzyme, DICER and the main miRNA effector, AGO2, are targeted for degradation as miRNA-free entities by the selective autophagy receptor NDP52. PMID: 23143396
  86. APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10 PMID: 22791714
  87. 2.3 angstrom resolution crystal structure of Ago2 reveals a bilobed molecule with a central cleft for binding guide and target RNAs; nucleotides 2 to 6 of a heterogeneous mixture of guide RNAs are positioned in an A-form conformation for base pairing with target messenger RNAs PMID: 22539551
  88. Study reports the structure of human Ago2 bound to a physiologically relevant microRNA, microRNA-20a, at 2.2 A resolution; the miRNA is anchored at both ends by the Mid and PAZ domains and makes several kinks and turns along the binding groove and miRNA binding confers remarkable stability on hAgo2. PMID: 22682761
  89. the nuclear Ago2 regulation of the K+ channel or stemness-related gene expression plays a critical role in adult stem cell self-renewal and differentiation. PMID: 22014067
  90. results provide novel evidence that Ago2 are probably involved in the pathobiology of human smooth muscle neoplasms PMID: 22394132
  91. Data show that expression of RB1 repressed both the wild-type and let-7-mutated promoter. PMID: 22366686
  92. Here the authors identified the N domain of human AGO2 as the initiator of duplex unwinding during RISC assembly. PMID: 22233755
  93. miR-122 binds hepatitis c viral RNA in association with Ago2 and that this slows decay of the viral genome in infected cells. PMID: 22215596
  94. Knock-down of argonaute 2 (AGO2) induces apoptosis in myeloid leukaemia cells. PMID: 21535412
  95. miR-671 directs cleavage of a circular antisense transcript of the Cerebellar Degeneration-Related protein 1 (CDR1) locus in an Ago2-slicer-dependent manner. PMID: 21964070
  96. Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2 PMID: 21969601
  97. Autoantigen La promotes efficient RNAi, antiviral response, and transposon silencing by facilitating multiple-turnover RNA-induced silencing complex catalysis. PMID: 22055194
  98. hAgo2 is recruited to stress granules as a consequence of its interaction with miRNAs. PMID: 21951848
  99. reveal that replication of hepatitis C virus (HCV) RNA depends on recruitment of Ago2 and miRNA-122 to lipid droplets, while suppression of HCV RNA by siRNA and Ago2 involves interaction with P-bodies PMID: 21868483
  100. Study reveals that Ago2, but not Ago2F2V2, inhibits the binding of CBP80/20 to cap structure. PMID: 21840310

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Subcellular Location Cytoplasm, P-body, Nucleus
Protein Families Argonaute family, Ago subfamily
Database Links

HGNC: 3263

OMIM: 606229

KEGG: hsa:27161

STRING: 9606.ENSP00000220592

UniGene: Hs.660189

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