AOX1A Antibody

Code CSB-PA655106LA01DOA
Size US$166
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  • Western Blot
    Positive WB detected in: Arabidopsis thaliana leaf tissue, Solanum lycopersicum leaf tissue, Glycine max leaf tissue, Solanum tuberosum leaf tissue
    All lanes: AOX1A antibody at 1:1500
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 40 kDa
    Observed band size: 40 kDa

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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana AOX1A Polyclonal antibody
Uniprot No.
Target Names
AOX1A
Alternative Names
AOX1A; AOX1; HSR3; At3g22370; MCB17.11; Ubiquinol oxidase 1a, mitochondrial; Alternative oxidase 1a
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana Ubiquinol oxidase 1a, mitochondrial protein (63-178AA)
Immunogen Species
Arabidopsis thaliana
Conjugate
Non-conjugated

The AOX1A Antibody (Product code: CSB-PA655106LA01DOA) is Non-conjugated. For AOX1A Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA655106LB01DOA AOX1A Antibody, HRP conjugated ELISA
FITC CSB-PA655106LC01DOA AOX1A Antibody, FITC conjugated
Biotin CSB-PA655106LD01DOA AOX1A Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalyzes the cyanide-resistant oxidation of ubiquinol and the reduction of molecular oxygen to water, but does not translocate protons and consequently is not linked to oxidative phosphorylation. Increases respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.
Gene References into Functions
  1. AOX1A activation by Tricarboxylic acid cycle Intermediates 2-oxoglutarate and oxaloacetate. PMID: 29208641
  2. AOX isoforms are differentially activated and that activation at CysI and CysII is additive. PMID: 28596420
  3. MYB DOMAIN PROTEIN29 (MYB29) mutants have increased levels of ALTERNATIVE OXIDASE1a (AOX1a) transcript and protein compared to wild type after induction with antimycin A. PMID: 28167700
  4. Overexpression of AOX1a rescued the aox1a mutant phenotype, including the chlorophyll accumulation during greening and plastidial protein import. PMID: 25158995
  5. Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings. PMID: 26163703
  6. AOX1A plays a significant role in sustaining the chloroplastic redox state and energization to optimize photosynthesis by regulating cellular redox homeostasis and ROS generation when electron transport through the COX pathway is disturbed at complex III. PMID: 26292995
  7. The authors propose a working model where AOX1a acts early in the response to Cd and activates or maintains a mitochondrial signalling pathway impacting on cellular antioxidative defence at the post-transcriptional level. PMID: 25743159
  8. RAO2/Arabidopsis NAC domain-containing protein17 (ANAC017) as a direct positive regulator of AOX1a. Plants with mutated rao2/anac017 were more stress sensitive. PMID: 24045017
  9. Arabidopsis plants deficient in AOX1a were unable to sustain photosynthesis under high light as is the case in WT plants. PMID: 24560882
  10. Overexpression of AOX1a reduced mitochondrial ROS production by maintaining the mitochondrial electron flux, and alleviated subsequent mitochondrial dysfunction and caspase-3-like activation in Al-induced programmed cell death. PMID: 24863436
  11. Foliar NO3 (-) assimilation was enhanced in both aox1a and ucp1 compared with the wild-type, suggesting that foliar NO3 (-) assimilation is probably driven by a decreased capacity of mAET and an increase in reductant within the cytosol. PMID: 24799562
  12. AOX and CET-PSI participate together in optimizing plant growth under conditions with elevated light intensity. [AOX1A] PMID: 21707657
  13. These results indicate that AOX is important for optimizing rates of photosynthetic CO(2) assimilation in response to rising CO(2) concentration. PMID: 22848123
  14. The ability of AOX1a and AOX2 to substitute for PTOX in the correct physiological and developmental contexts is a striking example of the capacity of a mitochondrial protein to replace the function of a chloroplast protein. PMID: 22534126
  15. These results suggest that AOX plays an important role in rapid acclimation of the respiratory chain to sHL, which may support efficient photosynthetic performance. PMID: 21251020
  16. When aox1a mutant seedlings were grown under a high-light condition, photobleaching was more evident in the mutant than the wild-type plants. PMID: 20716069
  17. AOX can play a critical role in cell re-programming under salinity stress. PMID: 19941623
  18. A 93 base pair portion necessary for induction was identified in the AtAOX1a promoter. PMID: 16027972
  19. AOX not only functions to prevent excess reactive oxygen species formation in whole tissues under stressful environmental conditions but also affects metabolism through more pervasive effects, including some that are extramitochondrial. PMID: 16299170
  20. a microarray study using an anti-sense line showed AOX1a influences outside mitochondria, particularly in chloroplasts and on several carbon metabolism pathways PMID: 16299171
  21. A variety of Cys(II) substitutions constitutively activated AtAOX1a, indicating that neither the catalytic site nor, unlike at Cys(I), charge repulsion is involved. PMID: 16457775
  22. AOX1a plants have a greatly altered stress response even when mitochondria or the mitochondrial electron transport chain are not the primary target of the stress and that AOX1a plays a broad role in determining the normal redox balance in the cell PMID: 18424626
  23. AOX is a necessary component in antioxidant defence mechanisms and for the control of a balanced metabolism. PMID: 18507803

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Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein. Note=Mitochondrial, possibly in the inner surface of the inner mitochondrial membrane.
Protein Families
Alternative oxidase family
Tissue Specificity
Expressed in roots, stems, cotyledons, leaves and flowers. High expression in sepals.
Database Links

KEGG: ath:AT3G22370

STRING: 3702.AT3G22370.1

UniGene: At.23475

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