FOXP1 Antibody

Datasheet
Code CSB-PA008841EA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Jurkat whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate
    All lanes: FOXP1 antibody at 4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 76, 68, 77, 13, 67, 78 kDa
    Observed band size: 90 kDa

  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA008841EA01HU at dilution of 1:100

  • Immunofluorescence staining of Hela cells with CSB-PA008841EA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-FOXP1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP008841HU) against the HSP90B promoter.

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) FOXP1 Polyclonal antibody
Uniprot No. Q9H334
Target Names FOXP1
Alternative Names Forkhead box protein P1 (Mac-1-regulated forkhead) (MFH), FOXP1
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Forkhead box protein P1 protein (1-114AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Transcriptional repressor
Involvement in disease Mental retardation with language impairment and autistic features (MRLIAF)
Subcellular Location Nucleus
Tissue Specificity Isoform 8 is specifically expressed in embryonic stem cells.
Database Links

HGNC: 3823

OMIM: 605515

KEGG: hsa:27086

STRING: 9606.ENSP00000318902

UniGene: Hs.59368

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