FOXP1 Antibody

Datasheet

Code CSB-PA008841EA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q9H334
Image
  • Western Blot
    Positive WB detected in: Jurkat whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate
    All lanes: FOXP1 antibody at 4μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 76, 55, 66, 75, 13, 67, 68 kDa
    Observed band size: 85 kDa

  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA008841EA01HU at dilution of 1:100

  • Immunofluorescence staining of Hela cells with CSB-PA008841EA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Chromatin Immunoprecipitation Hela(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-FOXP1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP008841HU) against the HSP90B promoter.

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
    All lanes: FOXP1 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 76, 55, 66, 75, 13, 67, 78 kDa
    Observed band size: 76 kDa

Immunogen Recombinant Human Forkhead box protein P1 protein (1-114AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:50-1:500
Relevance Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Forkhead box protein P1 (Mac-1-regulated forkhead) (MFH), FOXP1
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names FOXP1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcriptional repressor
Involvement in disease Mental retardation with language impairment and autistic features (MRLIAF)
Subcellular Location Nucleus
Tissue Specificity Isoform 8 is specifically expressed in embryonic stem cells.
Database Links

HGNC: 3823

OMIM: 605515

KEGG: hsa:27086

STRING: 9606.ENSP00000318902

UniGene: Hs.59368

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