PIAS3 Antibody

Code CSB-PA896765DSR1HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA896765DSR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA896765DSR1HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PIAS3 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
E3 SUMO protein ligase PIAS 3 antibody; E3 SUMO protein ligase PIAS3 antibody; E3 SUMO-protein ligase PIAS3 antibody; FLJ14651 antibody; OTTHUMP00000015586 antibody; OTTHUMP00000015587 antibody; PIAS 3 antibody; Pias3 antibody; PIAS3 protein antibody; PIAS3_HUMAN antibody; Protein inhibitor of activated STAT 3 antibody; Protein inhibitor of activated STAT protein 3 antibody; Protein inhibitor of activated STAT3 antibody; Zinc finger MIZ type containing 5 antibody; ZMIZ 5 antibody; ZMIZ5 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human E3 SUMO-protein ligase PIAS3 protein (399-628AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus.
Gene References into Functions
  1. PAI-1 interacted with PIAS3 to regulate Stat3-dependent gene expression and miR-34a was transcriptionally suppressed by Stat3 to form a positive regulatory loop through Stat3 signaling in non-small cell lung cancer cells. PMID: 28988111
  2. Levels of PIAS3 are significantly lower, in contrast with phosphorylation of STAT3, in women with endometriosis compared to women without endometriosis. PMID: 27226311
  3. TRIM8 activates STAT3 by suppressing the expression of PIAS3, an inhibitor of STAT3, most likely through E3-mediated ubiquitination and proteasomal degradation. PMID: 28100038
  4. Low PIAS3 expression is associated with breast cancer organoid invasiveness. PMID: 28423498
  5. SHP2, SOCS3 and PIAS3 levels are reduced in medulloblastomas in vivo and in vitro, of which PIAS3 downregulation is more reversely correlated with STAT3 activation. In resveratrol-suppressed medulloblastoma cells with STAT3 downregulation and decreased incidence of STAT3 nuclear translocation, PIAS3 is upregulated, the SHP2 level remains unchanged and SOCS3 is downregulated. PMID: 28035977
  6. The results revealed that although the expression levels of SOCS1, SOCS3 and, in particular, pSHP2, tend to decrease in the four types of astrocytomas, PIAS3 downregulation is more negatively correlated with STAT3 activation in the stepwise progress of astrocytomas and would indicate an unfavorable outcome. PMID: 28035384
  7. 3-Formylchromone inhibits proliferation and induces apoptosis of multiple myeloma cells by abrogating STAT3 signaling through the induction of PIAS3. PMID: 27324722
  8. PIAS3 may serve as a biomarker for predicting hormone therapy stratification, although it is limited to those breast cancer patients receiving hormone therapy. PMID: 26768588
  9. Taken together, these results suggest that PIAS3 functions as a positive regulator of HIF-1alpha-mediated transcription by increasing its protein stability. PMID: 26697750
  10. PIAS3 suppression may be protective against joint destruction in rheumatoid arthritis by regulating synoviocyte migration, invasion, and activation. PMID: 26667168
  11. PIAS3 primes ATR for checkpoint activation. PMID: 26565033
  12. PIAS3 expression in squamous cell lung cancer is low and predicts overall survival PMID: 25573684
  13. Adenovirus E4-ORF3 targets PIAS3 and together with E1B-55K remodels SUMO2/SUMO3 Interactions in the nucleus and at virus genome replication domains. PMID: 26223632
  14. Statistically significant positive correlation has been found between STAT5B and COX-2, and significant negative correlation between STAT5B and PIAS3. PMID: 25137041
  15. Low PIAS3 expression in malignant mesothelioma is associated with increased STAT3 activation and poor patient survival. PMID: 25124686
  16. this newly identified p53PIAS3 interaction through the 1-52 amino acid region of p53, reduces p53MDM2 complex formation, which not only increases the half-life of p53, but also its transactivation of target genes. PMID: 24584189
  17. PIAS3 may be a potential biomarker target for early cancer detection and therapeutic of human colorectal cancer. PMID: 24120699
  18. PIAS3 activates the intrinsic apoptotic pathway in non-small cell lung cancer cells independent of p53 status. PMID: 23959540
  19. Downregulation of PIAS3 is associated with gastric adenocarcinogenesis. PMID: 23322197
  20. PIAS3 expression is closely related to the invasion properties of glioma TJ905 cells. PMID: 21733403
  21. NF-kappaB is regulated through a novel negative feedback mechanism by SUMOylation, where the RelA subunit of NF-kappaB is SUMOylated by PIAS3. PMID: 22649547
  22. PIAS3 is a novel regulator of ErbB4 receptor tyrosine kinase, controlling its nuclear sequestration and function. PMID: 22584572
  23. Squamous cell carcinoma of the lung commonly lacks PIAS3 protein expression. PMID: 21497567
  24. novel transcription factor binding partners for PIAS3 including ETS, EGR1, NR1I2, and GATA1 were identified. PMID: 21532337
  25. Identification of vimentin (vimentin354), a nuclear component in glioblastoma multiforme cells, as the main target of sumoylation promoted by PIAS3. PMID: 21317457
  26. SOCS-3 and PIAS-3 upregulation impairs IL-12-mediated interferon-gamma response in CD56 T cells in HCV-infected heroin users PMID: 20231901
  27. These findings indicate that TRIM8 enhances the STAT3-dependent signal pathway by inhibiting the function of PIAS3. PMID: 20516148
  28. we selected one coding single nucleotide polymorphism in PIAS3 for association analyses in patients with breast cancer PMID: 19760037
  29. there is an interaction between Zimp7 and PIAS proteins with higher preference for PIAS3, in androgen receptor-mediated transcription PMID: 20159969
  30. data suggest an important role for the negative regulatory effect of PIAS3 on STAT3 in EGF-driven tumors PMID: 19903771
  31. AR and PIAS3 regulate the STAT3-mediated transcriptional activity by their physical protein-protein competition on STAT3 PMID: 12804609
  32. Up-regulation of protein inhibitor of activated STAT3 protein is associated with adult T-cell leukemia PMID: 14630083
  33. PIAS3 and Smad3 interact with each other at the endogenous protein level in mammalian cells and also in vitro, and the association occurs through the C-terminal domain of Smad3. PMID: 14691252
  34. In conclusion, ATBF1 can suppress the IL-6-mediated cellular response by acting together with PIAS3. PMID: 14715251
  35. Increased PIAS3 expression is associated with human cancers including lung, breast, prostate, colon-rectum, and brain tumors PMID: 15138572
  36. PIAS3 may function in vivo as a modulator in suppressing the transcriptional activity of p65 PMID: 15140884
  37. End-stage hepatitis c and alcoholic liver cirrhosis is characterized by impaired Stat3 DNA-binding possibly through up-regulation of Pias3. PMID: 16098628
  38. Mechanisms of PIAS3 activity could pave the way toward the formulation of an antioncogenic agent for the inhibition of both STAT3 and MITF. PMID: 16368885
  39. overexpression of PIAS3 in lung cancer cells contributed to growth suppression and restored the drug sensitivity of the cells PMID: 17032498
  40. PIAS3 is a new regulator of ATF1 that regulates the ARE-mediated transcription of the ferritin H gene PMID: 17565989
  41. Both SUMO E2 conjugating enzyme Ubc9 and E3 ligase protein inhibitor of activated STAT3 (Pias3) are targets for S-nitrosation PMID: 17987106
  42. PIAS3 binds to Tel and stimulates sumoylation of K11 in the nucleus.PIAS3 may have a dual, context-dependent influence on Tel; it mediates Tel sumoylation, but it also augments Tel's repressive function in a sumoylation-independent fashion. PMID: 18212042
  43. parvovirus B19 NS1 protein modulates inflammatory signaling by activation of STAT3/PIAS3 in human endothelial cells PMID: 18550668
  44. This study suggests a link between PIAS3 and nuclear pyruvate kinase. PMID: 19308990
  45. PIAS3 is expressed in NSCLC cell lines and its over-expression decreased STAT3 transcriptional activity, decreased proliferation of NSCLC cells and when used in conjunction with EGFR inhibitors, increased the anti-proliferative effects. PMID: 19569236
  46. Pias3 associates and colocalizes with Trim32, a Ubiquitin E3 ligase. PMID: 17987106

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Subcellular Location
Cytoplasm. Nucleus. Nucleus speckle.
Protein Families
PIAS family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 16861

OMIM: 605987

KEGG: hsa:10401

STRING: 9606.ENSP00000376765

UniGene: Hs.435761

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