SULT1E1 Antibody

Code CSB-PA022941ESR2HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western blot
    All lanes: Estrogen sulfotransferase antibody at 7μg/ml
    Lane 1: Mouse lung tissue
    Lane 2: Mouse liver tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 35, 30 kDa
    Observed band size: 35 kDa

  • Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA022941ESR2HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) SULT1E1 Polyclonal antibody
Uniprot No. P49888
Target Names SULT1E1
Alternative Names EST antibody; EST-1 antibody; EST1 antibody; Estrogen sulfotransferase antibody; estrogen-preferring antibody; estrone sulfotransferase antibody; ST1E1 antibody; ST1E1_HUMAN antibody; STE antibody; Sulfotransferase 1E1 antibody; Sulfotransferase antibody; Sulfotransferase estrogen preferring antibody; Sulfotransferase family 1E member 1 antibody; SULT1E1 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Estrogen sulfotransferase protein (140-294AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:1000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Human estrogen sulfotransferase and its related fluorescently labeled decapeptides specifically interact with oxidized low‐density lipoprotein. A Sato,J. Pept. Sci,2020

Applications: WB
Review: The rabbit anti-hSULT1E1 polyclonal antibody was purchased from CUSABIO Technology LLC. Western blot of hSULT1E1 in the absence or presence of LDL/Ox-LDL.
PMID: 32633098

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Target Data

Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
Gene References into Functions
  1. Galeterone and abiraterone are novel inhibitors of DHEA sulfonation, as determined in enzymatic incubations containing human tissue cytosol (liver or intestinal) or human recombinant SULT enzyme (SULT2A1, SULT2B1b, or SULT1E1). PMID: 29436390
  2. Single nucleotide polymorphisms in SULT1E1 were significantly associated with time to treatment failure in men on abiraterone acetate therapy. The single nucleotide polymorphisms may serve as predictive markers for treatment with abiraterone acetate. PMID: 27150425
  3. Resveratrol and all its derivatives reduced also SULT1E1 mRNA transcript level. The reduced expression of AhR, CYP1A1, and 1B1 was also found as a result of treatment with these compounds. PMID: 27854074
  4. Data show that both estrogen sulfatase (STS) and estrogen sulfotransferase (EST) were highly expressed in the human umbilical vein PMID: 26458420
  5. Effects of steroid hormone on estrogen sulfotransferase and on steroid sulfatase expression in endometriosis tissue and stromal cells PMID: 26723541
  6. a model that enables prediction of substrates and inhibitors of SULT1E1, is reported. PMID: 26542807
  7. It metabolizes breast cancer drugs like afimoxifene and endoxifen by sulfation. PMID: 26169578
  8. Data suggest that the substrate specificities of SULT1E1 and SULT1A1*1 include metabolites of tamoxifen (endoxifen, 4-hydroxytamoxifen, and N-desmethyltamoxifen); these metabolites are weak inhibitors of sulfation of estradiol by SULT1E1/SULT1A1*1. PMID: 25819444
  9. Authors propose that the formation of this DNA loop and protein-bound complex prevents additional binding of ETS1 and p53 R273H proteins to other proximal binding sites. PMID: 24481480
  10. Data indicate that protein-ligand interaction energy by using docking Quantitative Structure-Activity Relationships(QSAR) models showed accuracy of 67.28%, 78.00% and 75.46%, for the isoforms SULT1A1, SULT1A3 and SULT1E1, respectively. PMID: 24039991
  11. Overexpression of EST promoted adipogenesis. PMID: 24567372
  12. Report diterpenoid extracts from Leonurus sibiricus L. with estrogen sulfotransferase inhibitory activity. PMID: 23736977
  13. Knock-down of SULT1E1 in HUVECs resulted in regulation of genes involved in inflammation and lipid metabolism. PMID: 23384540
  14. Decrease or deletion of EST in the mammary gland may be related to the development of hypertrophic breast, especially gland-associated hypertrophic breast. PMID: 21548389
  15. study showed expression patterns of SULT1E1 and PAPSS in breast and endometrial tissues; the estrogen sulfation enzymes were comparatively higher in the tumorous tissues than adjacent normal tissues; overexpression of SULT1E1 and PAPSS1 retarded MCF-7 cells growth in vivo and in vitro by arresting cell cycles and inducing apoptosis PMID: 22380844
  16. Using two iterative rounds of evolution, we generated SULT1E1 mutants with increased thermostability. PMID: 22197379
  17. results demonstrate that SULT1E1 expression in MCF10A breast epithelial cells cells is transcriptionally regulated by confluence through a suppressive action of the AhR, which is not mediated through a dioxin response element at nt -3476 PMID: 21828262
  18. Identification of the upstream enhancer of the SULT1E1 promoter that plays a crucial role in regulating the activity of SULT1E1 transcription. PMID: 21764778
  19. Recent results of STS and EST in several estrogen-dependent carcinomas, are summarised. PMID: 21073915
  20. estrogen sulfotransferase is expressed in abdominal sc adipose tissue of both obese males and females in association with expression of TNF-alpha and SOCS3, suggesting potential roles in inflammation. PMID: 21543429
  21. Human EST (a dimer of identical protomers) presents a clear example of half-sites reactivity--only one subunit of the dimer produces product during the catalytic cycle. It is the first example of half-sites reactivity in the sulfotransferase family. PMID: 20429582
  22. Murine SULT1E1 inhibition in vitro and in silico was investigated and compared this to data for the human enzyme. PMID: 20025931
  23. linkage to SULT1B1 and to a SULT1D1 pseudogene on chromosome 4 PMID: 11688987
  24. crystal structure of human EST (hEST) in the context of the V269E mutant-PAPS complex, which is the first structure containing the active sulfate donor for any sulfotransferase PMID: 11884392
  25. Levels of steroid sulfatase and STS mRNA and activity may be significantly associated with degree of atherosclerotic changes in female aorta, which may be related to cytokines produced in situ, such as IL-1beta, in human atherosclerotic lesions. PMID: 14507642
  26. estrogen-dependent cell growth of the EST-transfected cell clones was found to be abolished, due to the enhanced sulfoconjugation of estrogen PMID: 14556660
  27. Decreased estrogen sulfotransferase expression is associated with estrogen-dependent endometrial carcinomas PMID: 15355916
  28. Women with the SULT1E1 *959 GA/AA genotype had a moderately decreased breast cancer risk compared with those with the GG genotypes. They had an increased risk of recurrence compared with the TT geonotype PMID: 15894657
  29. Sulfation of resveratrol in human liver: evidence of a major role for the sulfotransferases SULT1E1. PMID: 16418064
  30. EST 1E1 mRNA and protein were found to be expressed in epithelial cells bordering alveola lumen (luminal cells) as well as stroma cells in the prostate PMID: 17187396
  31. Altered cellular proliferation was observed in cells stably expressing SULT1E1. PMID: 17293380
  32. Down-regulation of epithelial estrogen sulfotransferase was associated with high-grade ductal carcinoma in situ in breast cancer PMID: 17661084
  33. a decreased single nucleotide polymorphism of CYP1A1 and an increased single nucleotide polymorphism for SULT1A1 and SULT1E1 genes may be risk factors for endometrial cancer in Caucasians. PMID: 18318428
  34. Data show higher levels for EST mRNA were obtained in polycystic ovarian syndrome endometria without treatment versus normal endometria. PMID: 18467089
  35. Activation of glucocorticoid receptor by dexamethasone induced the activity of estrogen sulfotransferase (SULT1E1), an enzyme important for the metabolic deactivation of estrogens, because sulfonated estrogens fail to activate the estrogen receptor. PMID: 18794126
  36. Data show that SULT1E1 expression in HepG2 cells is inducible by sterol mediated liver-X-receptor (LXR) activation. PMID: 19429440
  37. SULT1E1 mRNA is expressed in human skin, at higher levels in men than women (3-4 times greater arm and hip). No gender difference in face skin. SUL1E1 mRNA levels are not altered by topical 17-beta-estradiol treatment. PMID: 18794456

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Subcellular Location Cytoplasm
Protein Families Sulfotransferase 1 family
Tissue Specificity Liver, intestine and at lower level in the kidney.
Database Links

HGNC: 11377

OMIM: 600043

KEGG: hsa:6783

STRING: 9606.ENSP00000226444

UniGene: Hs.479898

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