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Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein (IE1), partial

Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein (IE1), partial

Code	CSB-YP318109ARA
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP318109ARA
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP318109ARA-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP318109ARA
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP318109ARA
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

IE1

Uniprot No.

P11138

Alternative Names

IE1; Trans-activating transcriptional regulatory protein; Immediate early protein 1; IE-1

Species

Autographa californica nuclear polyhedrosis virus (AcMNPV)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Regulatory transcriptional protein, which trans-activates gene expression from early baculovirus promoters. Can also trans-activate its own promoter, suggesting an autoregulation during infection of host cells. Promotes also viral DNA genome replication via the N-terminal region.

Gene References into Functions

Phosphorylation sites in the IE1 replication domain are required for virus multiplication. PMID: 22496221
These results suggest that IE1 regulates gp64 early promoters to produce the proper amount of GP64 protein, depending upon nucleopolyhedrovirus-insect cell systems. PMID: 20837073
Ie1-deficient mutant abrogates viral gene expression and can not replicate in insect host cells. PMID: 20705310
We conclude that a unique cooperative mechanism of ie1 and p35 exists in the genome of AcMNPV, which can activate the expression of a specific set of AcMNPV and HzNV-1 promoters PMID: 18486255
This study reveals that IE1 is a gene-selective transcriptional activator which is required not only for expedition of virus multiplication but also for blocking of its own proapoptotic activity by upregulation of baculovirus apoptotic suppressors. PMID: 18945761

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Subcellular Location

Host nucleus.

Protein Families

Nucleopolyhedrovirus IE-1 protein family

Database Links

KEGG: vg:1403980

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