Recombinant Bacillus subtilis Probable NAD-dependent malic enzyme 4 (ytsJ)

Code CSB-YP522034BRJ
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP522034BRJ
MSDS
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Source E.coli
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Code CSB-EP522034BRJ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522034BRJ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP522034BRJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ytsJ
Uniprot No.
Alternative Names
ytsJ; BSU29220Probable NAD-dependent malic enzyme 4; NAD-ME 4; EC 1.1.1.38
Species
Bacillus subtilis (strain 168)
Expression Region
1-410
Target Protein Sequence
MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED INKVYDYTMK GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG VDAFPIALNT NDVDKIVETV KLLEPTFGGV NLEDIAAPNC FIIEERLKKE TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK SMSSIKVVAN GAGAAGIAII KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN QDRKDGSLKD VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS LVSEDELSAD YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE KTRKLTIIGE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Bifunctional enzyme with both malic and malolactic enzyme activities. In the absence of NADPH, catalyzes the reversible decarboxylation of malate to pyruvate. Can use NAD and NADP, but with a very strong preference for NADP. In the presence of excess NADPH, catalyzes the non-oxidative decarboxylation of malate to lactate. During growth on glucose, contributes to NADPH balancing via oxidation of the NADPH produced in excess by other enzymatic reactions. Can also catalyze the decarboxylation of oxaloacetate.
Protein Families
Malic enzymes family
Database Links
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