Recombinant Borrelia burgdorferi Nuclease sbcCD subunit D (sbcD)

Code CSB-YP526256BUD
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP526256BUD
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP526256BUD-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP526256BUD
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP526256BUD
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sbcD
Uniprot No.
Alternative Names
sbcD; BB_0829; Nuclease SbcCD subunit D
Species
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Expression Region
1-413
Target Protein Sequence
MSNYKILHTS DWHIGKKIEN FSILKEQKNF LYFLLEFIKK ENIDLLLVAG DVYDSKRPGF EEQRLVNNFF YELSFTSCKW CVVISGNHDK KDYLSINKKL LSRFNFFLIT EYDSDEQIVL LKDNGNLKFI VVCLPHINER LILGQNFDNI FGLEDQYSSK LFLENLENAY REKISNLSNF LENKYKGIPK ILMAHSFFGS SKKIDTLGGS YIIPFNVFGN GFSYVALGHI HKFMKLRDNI VYSGSPMQYS FNETCDKYIN VLHFNDNKLI LQEAFPVPIF NKLIFAKGSL NEVLDFLANS KKEESFTIYL KIELNEAVDT SAEESIYDLA RLNFMNLVSI SYSLPSSQDL QDDSNFIGEL EVLEMDEKYF FEKKLRWDFE NGVIRDIKFK EEELISLFNE VLANGYLGEY EDK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.
Protein Families
SbcD family
Database Links

KEGG: bbu:BB_0829

STRING: 224326.BB_0829

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