Recombinant Bothrops jararacussu Galactose-specific lectin

Code CSB-YP307365BUX
MSDS
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Source Yeast
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Code CSB-EP307365BUX
MSDS
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Source E.coli
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Code CSB-EP307365BUX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP307365BUX
MSDS
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Source Baculovirus
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Code CSB-MP307365BUX
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
C-type lectin BJcuL; CTL; Galactose-specific lectin; Snake venom galactoside-binding lectin; SVgalL
Species
Bothrops jararacussu (Jararacussu)
Expression Region
25-159
Target Protein Sequence
NNCPQD WLPMNGLCYK IFNELKAWKD AEMFCRKYKP GCHLASIHLY GESPEIAEYI SDYHKGQSEV WIGLCDKKKD FSWEWTDRSC TDYLSWDKNQ PDHYQNKEFC VELVSNTGYR LWNDQVCESK NAFLCQCKF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Galactose-binding lectin which recognizes specific carbohydrate structures and agglutinates a variety of animal cells by binding to cell-surface glycoproteins and glycolipids. Calcium-dependent lectin. Also binds lactose and raffinose. Shows high hemagglutinating activity on mammalian erythrocytes (Ref.5, PubMed:16309723). It also involved in immunological functions, since it is able of inducing potent neutrophil activation. In vivo, it causes edema and increases vascular permeability after injection into mouse hind paws (10-100 ug/paw). In anesthetized rats, it decreases the blood pressure by approximately 15%, with a rapid return to the resting level. Is an effective inhibitor of cell growth in some cancer cell lines, especially against renal and pancreatic cancer cell lines, human breast and ovarian carcinoma, glioblastoma and a bovine brain microvascular endothelial cell line.
Subcellular Location
Secreted.
Protein Families
True venom lectin family
Tissue Specificity
Expressed by the venom gland.
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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