Recombinant Catharanthus roseus Tabersonine 16-hydroxylase (CYP71D12)

Code CSB-YP311050DQN
MSDS
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Source Yeast
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Code CSB-EP311050DQN
MSDS
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Source E.coli
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Code CSB-EP311050DQN-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP311050DQN
MSDS
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Source Baculovirus
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Code CSB-MP311050DQN
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP71D12
Uniprot No.
Alternative Names
CYP71D12; T16H1Tabersonine 16-hydroxylase 1; EC 1.14.14.103; Cytochrome P450 71D12
Species
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Expression Region
1-495
Target Protein Sequence
LLFCFILSKT TKKFGQNSQY SNHDELPPGP PQIPILGNAH QLSGGHTHHI LRDLAKKYGP LMHLKIGEVS TIVASSPQIA EEIFRTHDIL FADRPSNLES FKIVSYDFSD MVVSPYGNYW RQLRKISMME LLSQKSVQSF RSIREEEVLN FIKSIGSKEG TRINLSKEIS LLIYGITTRA AFGEKNKNTE EFIRLLDQLT KAVAEPNIAD MFPSLKFLQL ISTSKYKIEK IHKQFDVIVE TILKGHKEKI NKPLSQENGE KKEDLVDVLL NIQRRNDFEA PLGDKNIKAI IFNIFSAGTE TSSTTVDWAM CEMIKNPTVM KKAQEEVRKV FNEEGNVDET KLHQLKYLQA VIKETLRLHP PVPLLLPREC REQCKIKGYT IPSKSRVIVN AWAIGRDPNY WIEPEKFNPD RFLESKVDFK GNSFEYLPFG GGRRICPGIT FALANIELPL AQLLFHFDWQ SNTEKLNMKE SRGVTVRRED DLYLTPVNFS SSSPA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the flower biosynthesis of vindoline, a precursor of vinblastine and vincristine. Hydroxylates specifically tabersonine, 2,3-dihydrotabersonine and 2,3-dihydro-3-hydroxytabersonine, but has no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline and catharanthine.
Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Predominantly expressed in young leaves of mature plants. Low expression in roots and flowers, but not detected in stems and old leaves. Found predominantly in leaf epidermis. Barely detected in roots, internodes, young and mature leaves, and flower buds,
Database Links

KEGG: ag:CAB56503

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