Recombinant Chicken Alkaline phosphatase, tissue-nonspecific isozyme (ALPL)

Code CSB-YP835615CH
MSDS
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Source Yeast
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Code CSB-EP835615CH
MSDS
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Source E.coli
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Code CSB-EP835615CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835615CH
MSDS
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Source Baculovirus
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Code CSB-MP835615CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ALPL
Uniprot No.
Alternative Names
ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; EC 3.1.3.1
Species
Gallus gallus (Chicken)
Expression Region
17-498
Target Protein Sequence
LVPE REKDPEYWRQ QAQETLRDAL RLQHLNQNVA KNLILFLGDG MGVSTVTAAR ILKGQLQHRK GEESLLEMDK FPYVALAKTY NTNAQVPDSA GTATAYLCGV KANEGTVGVS AGVTRDRCNT TKGQEVTSIL RWAKDEGKAV GIVTTTRVTH ATPSAAYAHS ANRDWYSDGE MPLDALEGGC KDIARQLVDN IPDIEVILGG GRKYMFPKNT SDVEYPQEER HRGTRLDGKD LVQAWHDTKP AGKVAKYVWH RRELLALNVS RVDFLLGLFE PGDMVYELDR NNETDPSLSE MVAVAIRMLQ KNPRGFFLLV EGGRIDHGHH EGKAKQALHE AVELDRAVGL AGRLTSPRDT LSVVTADHSH VFTFGGYTPR GNPIFGLAPM QSDVDRKPFT SILYGNGPGY KIVGGERENV SAVDFAHANY QAQAAVPLRQ ETHGGEDVAV FARGPMAHLL HGVDEQNYIP HAMAYAACIG PNRAHCSS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi) and pyridoxal 5'-phosphate (PLP) are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters. Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors. Involved in the establishment and growth of feather germs.
Gene References into Functions
  1. Cooperative functions of ZnT1, metallothionein and ZnT4 in the cytoplasm are required for full activation of TNAP in the early secretory pathway. PMID: 24204829
  2. Tissue-nonspecific alkaline phosphatase (in matrix vesicles of chick embryos) is inhibited by lansoprazole (a noncompetitive inhibitor). PMID: 19543559
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Extracellular vesicle membrane; Lipid-anchor, GPI-anchor.
Protein Families
Alkaline phosphatase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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