Recombinant Chicken Melanocyte protein PMEL (PMEL), partial

Code CSB-YP836538CH
MSDS
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Source Yeast
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Code CSB-EP836538CH
MSDS
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Source E.coli
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Code CSB-EP836538CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836538CH
MSDS
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Source Baculovirus
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Code CSB-MP836538CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PMEL
Uniprot No.
Alternative Names
PMEL; MMP115; PMEL17; SILV; Melanocyte protein PMEL; 115 kDa melanosomal matrix protein; Melanocyte protein Pmel 17; Premelanosome protein; Silver locus protein homolog
Species
Gallus gallus (Chicken)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Might be required for polymerization of melanin onto the core structure of melanosomes with enzymatic function of tyrosinase.
Gene References into Functions
  1. The study strongly suggests a correlated effect between plumage colour and behaviour, and we conclude that PMEL17 may have a pleiotropic effect on social and explorative behaviour in chickens. PMID: 20461577
  2. the transmembrane region may contribute to the structural integrity of Silver/Pmel17 oligomers or influence toxic, amyloidogenic properties PMID: 19679373
  3. Dominant white is exclusively associated with a 9-bp insertion in exon 10, Dun is associated with a deletion of 15-bp in exon 10, and Smoky has the same 9-bp insertion as Dominant white as well as a 12-bp deletion in exon 6 of PMEL17. PMID: 15579702
  4. Genotype of PMEL17 affects plumage color and feather pecking behavior. PMID: 17106652
  5. In addition to the 9-bp insertion, WLs and KOCs have unique bases in single nucleotide polymorphisms (SNPs) at the 1,777th and 3,118th bases in the PMEL17 gene. PMID: 17436332
  6. Dominant white mutation does not cause visual impairment PMID: 19751488

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Subcellular Location
Melanosome lumen. Note=On the fibrous matrix structure of the premelanosome.
Protein Families
PMEL/NMB family
Tissue Specificity
Specific to pigmented epithelial cells and melanocytes. Not expressed in lens, neural retina, brain, heart, gizzard or liver.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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