Recombinant Chicken Phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1)

Code CSB-YP017938CH
MSDS
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Source Yeast
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Code CSB-EP017938CH
MSDS
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Source E.coli
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Code CSB-EP017938CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017938CH
MSDS
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Source Baculovirus
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Code CSB-MP017938CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PHOSPHO1
Uniprot No.
Alternative Names
PHOSPHO1; Phosphoethanolamine/phosphocholine phosphatase; EC 3.1.3.75; 3X11A
Species
Gallus gallus (Chicken)
Expression Region
1-268
Target Protein Sequence
MKRCCEGVGL PCLFKGVGMA SSRPPKYLLV FDFDGTIINE SSDDSIVRAA PGQALPEHIR QSFREGFYNE YMQRVLAYMG DQGVKMGDFK AVYENIPLSP GMPDLFQFLS KNHELFEIIL ISDANMFGIE CKLRAAGFYS LFRKIFSNPS SFDKRGYFTL GPYHSHKCLD CPANTCKRKI LTEYLAERAQ EEVEFERVFY VGDGANDFCP SVTLTSADVA FPRKGYPMHQ MTQEMEKKQP GTFQATVVPW ESATEVARYL QELLKKKC
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
Gene References into Functions
  1. In conclusion, this study has disclosed that PHOSPHO1 expression mirrors that of TNAP during embryonic bone development and that PHOSPHO1 contributes to bone mineralization in developing chick long bones. PMID: 20053388
  2. PHOSPHO1-a member of the large haloacid dehalogenase superfamily of hydrolases-is localized to sites of mineralisation in both cartilage and bone PMID: 15050893
Subcellular Location
Extracellular vesicle.
Protein Families
HAD-like hydrolase superfamily, PHOSPHO family
Tissue Specificity
Expressed at sites of mineralization in bone and cartilage. Highly expressed in hypertrophic chondrocytes compared to non-chondrogenic tissues. Expressed in chondrocytes but not in heart, liver, lung, kidney, spleen, muscle, adipose tissues not duodenum.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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