Recombinant Chicken Programmed cell death protein 4 (PDCD4)

Code CSB-YP857423CH
MSDS
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Source Yeast
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Code CSB-EP857423CH
MSDS
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Source E.coli
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Code CSB-EP857423CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP857423CH
MSDS
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Source Baculovirus
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Code CSB-MP857423CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PDCD4
Uniprot No.
Alternative Names
PDCD4; Programmed cell death protein 4; Protein I11/6
Species
Gallus gallus (Chicken)
Expression Region
1-467
Target Protein Sequence
MEIEKQHVYI STVEVENLSD ALFSGDEENG GSEERKTEIN GNWIPATSIT EAKINAKAKR RLRKNSSRDS GRGDSVSENG ETQKAGLVVP TSPKGKVLDR RSRSGKGRGL PKKGGAGGKG VWGTPGQVYD VEEVDIKDPN YDDDQENCVY ETVVLPLDER AFEKTLTPII QEYFEHGDTN EVSEMLKDLN LGEMKYSVPV LAVSLALEGK ASHREMTSKL ISDLCGTVVS KTDVEKSFDK LLKDLPDLVL DSPRAPQLVG QFIARAVGDG ILSSTYIDGY KGTVDSIQAR AALDRATVLL SVTKGGKRID NVWGSGGGQQ SVKHLVKEID MLLKEYLLSG DLLEAERCLQ ELEVPHFHHE LVYEAIVMVL ESTGEKTFKM MLDLLKSLSR SSVITMDQMK RGYERVYCEI PDINLDVPHS YSVLERFVEE CFQAGIISKP LRDLCPSRGR KRFVSEGDGG RLKPESY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A and EIF4G. Inhibits the helicase activity of EIF4A. Binds RNA. Does not seem to be involved in apoptosis.
Gene References into Functions
  1. This work reveals a novel mechanism by which Pdcd4 affects the translation of cellular RNAs. PMID: 24681950
  2. c-myb mRNA as the first natural translational target mRNA of Pdcd4 PMID: 21643008
  3. Pdcd4 has an important function in the cellular response to DNA damage PMID: 19684621
Subcellular Location
Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions.
Protein Families
PDCD4 family
Tissue Specificity
Expressed in a broad spectrum of hematopoietic organs, such as thymus and bursa. Lower levels of expression detected in the kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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